autoclave (primus)

STLCC-CPLS:Morrison 5/27/2015

Prepared by: Bob Morrison

STLCC, Instrumentation Specialist

Dec 08, Last revision May 2015 (microburner)

Primus Model A Sterilizer

W RO water feed

@BRDG

Glasswasher @ BRDG

Tuttnauer Model 5596 SP-1

@ FV

Bacticinerators and Bead

Autoclave & Sterilizers (Detect Instrument Picture to Jump to that section of SOP)

Micro-Burner, Butane

Glasswasher; @BRDG, VWR


Feature stainless steel construction for long life and corrosion protection.

Multiple cycle selections and microprocessor control ensure that each load is processed

efficiently and with the minimum energy required to meet cleaning requirements.

Operation is simple and loading is easy and flexible. A delayed-start feature allows the

washers to operate automatically unattended during off-peak hours.

Uses a maximum of 15L (3.9gal.) of tap water. Low water requirement and precise

detergent and neutralizer dosing minimize chemical costs

Spindle-ready models use an existing deionized water supply and can accommodate

either one or two direct injection spindle racks to clean up to 44 flasks.

Volume: 170 L (6 cu. ft.)

Exterior Dimensions,

Undercounter Unit: 61W x 62D x 87–93H cm (24 x 241/2 x 341/4–361/2")

Freestanding/Mobile Unit: 61W x 62D x 97.5H cm (24 x 241/2 x 383/8")(minimum height)

Interior Dimensions: 55 x 48 x 64 cm (211/2 x 19 x 251/4")

Clearance,

Standard Upper Open Rack: 25.7 cm (101/8")

Standard Lower Open Rack: 30.5 cm (12")

Spindle Rack-Ready Upper Rack*: 19.7 cm (73/4")

Spindle Rack-Ready Lower Rack*: 26 cm (101/4")

Electrical: 115V, 60Hz, 15A

*Maximum height with spindle clip at lowest position on spindle tube.

Washer with DI Rinse and DI Pump, Spindle-Ready 97021-364

Link to Manf website, Optional equipment, details….

http://www.hotpack.com/category/2186

Glasswasher: Control Panel, Detergent, @BRDG


START; press

Stop; Hold >3 sec

High Heat Rinse

Button and LED High Heat Wash

Button and LED

Delayed Start and

LED (above)

PROG: Press to

set cycles; Max to

Rinse only

LED Displays Time

remaining in cycle

and current Temp.

MASTER ON/OFF

(Open Door to access)

Detergent;

Load main

and prewash

cups if

desired

Glasswasher: Cycles/Phases, Settings


Autoclave: Sterilizer, BRDG, Primus, Clinical Model A


Link to Primus Clinical –

Small Size Spec Sheet .pdf

Loading cart/gurney

Install date @BRDG 1/14/10 B-E Hospital Equipment Co, Inc.

Clinical Model A, SN 17413, Primus Sterilizer Co.

www.primus-sterilizer.com

316L Stainless Steel Chamber with Standard PriMirror

Interior Surface , Dimensions: 20”x20”x38”

PSS500 Microcomputer Control

Standard Vacuum, Gravity, Liquid and Test Cycles

Single, Manual, Vertical Sliding Door

Electric Steam Boiler, 208V, #4 ACW, 4 wire, 3ph, 67amps

Cybertech Thermal Printer with Take Up Reel

Drain Water Conservation Quench Includes Air Compressor

Safety Gloves

Emergency

Power OFF

Service:

636-343-8928

users.stlcc.edu/Departments/fvbio/Autoclave_BRDG_Primus_ClinicalSpecsheet.pdf

Autoclave: BRDG, Primus, MTC Services Sep 2013


Clinical Model A, SN 17413, Primus Sterilizer Co.

www.primus-sterilizer.com

Alternative Services

CDS or ACE Lab Systems

Autoclave: Primus, BRDG, Control Panel, Defining Cycles


Press buttons 1-4 to

choose those cycles.

Press up-arrow and

then 1-4 buttons to

access cycles 5-8

Changing Cycle Parameters:

1. Press Cycle to be changed, then press

MENU key, LED will light.

2. To change CycleType, press Enter,

then up arrow to see all types, press

Enter again to select a type.

3. To change other parameters on this

Cycle, use up arrow to scroll to the

next cycle parameter to be modified.

4. To change that value, Press Enter key,

“asterisk” key then lights up.

5. Use up/down arrows to change the

value (must be within allowable range

see pg 14)

6. Press ENTER key when done, then

continue up arrow to next parameter

7. Press ADV key to exit and return to

the idle screen for normal operations.

ADV Key; resets

any operation

(Exit)

OFF Key, press

to stop

operations

Lights: Indicate

current Cycle

phase or activity

Link to Primus Operator Manual .pdf

Displays current

state or cycle

and parameters

users.stlcc.edu/Departments/fvbio/Autoclave_BRDG_Primus_Manual.pdf

Autoclave: Starting a Cycle, Primus, BRDG


STARTING A CYCLE, be patient

1. Press the keypad for the desired cycle, the

LED for that pad will turn on. For cycles

above 4, press the up arrow and keypad

numbers 1-4 (cycles 5-8)

2. Close the Sterilizer Door. You should see

confirmation with the Door Closed LED.

3. Wait ..…..The “START” button be not be

operative until the jacket temperature is

within 6 F of the cycle operating

temperature.

4. To view the Jacket temperature, press the

Upper left hand heart on the Primus

symbol.

5. When the jacket temperature has been

reached, the READY and START LEDs will

be lit.

6. Press the START button and hold for a few

seconds to begin the cycle. The READY

and START LED indicators will go out,

other cycle indicator LEDs will follow the

Cycle as it runs.

Door button: may

be required to

release door after

a cycle

Autoclave: Primus, Typical Cycle Diagram, Liquids


Autoclave: Primus, BRDG Modified Cycles


0

Autoclave: Primus, Gravity Cycle Description


Autoclave: Primus, Liquids Cycle Description


Autoclave: Primus, Vacuum Cycle Description


Autoclave: Primus, Cycle Sequences


Autoclave: Primus, Parameter Range


Autoclave: Safety Guidelines, Primus, BRDG


1. Never overload the Sterilizer, sterilization may never be achieved.

2. LIQUID cycles are only for the sterilization of Liquids, DO NOT USE

ANY OTHER CYCLE for Liquids.

3. When processing LIQUIDS, use containers with vented closures..do not use

ordinary glass bottles or any containers not designed for this purpose.

4. NEVER sterilize sealed containers in a cycle other than a LIQUIDS

cycle, rapid pressure changes in other cycles can cause explosive results.

5. NEVER obstruct the drains in the bottom of the chamber, always load in a

manner where no objects will touch or move to chamber walls.

6. ALWAYS use “hot gloves” when loading or unloading the sterilizer,

before or after a cycle…. ALWAYS assume the door, walls, racks,

and/or gurney are HOT!

7. ALWAYS place “hot gloves” on the ends of a loading cart

to indicate to others that the cart and labware are HOT!

Autoclave: Primus, BRDG,

Maintenance, Blow-down, Weekly (per Wally 11/28/11)


WEEKLY – Execute this BLOW OUT protocol to

maintain boiler when unit is at operating

condition

1. Turn OFF boiler (toggle switch on rear of boiler)

2. Turn OFF mail 208V power switch (left front wall)

3. Turn OFF autoclave (control panel)

4. Turn OFF Main water supply valve at rear wall

5. Unplug all three 110V power cords at rear

6. Remove Right side Autoclave Stainless Panel

7. Using a protective glove locate Blow-Out Valve

(Green) at lower rear

8. Open valve slowly about 30 degrees

counterclockwise

9. Observe water/steam exiting to drain on wall

behind Millipore…. Wait until steam subsides

10. Open valve another 30 degrees, wait, and repeat

again until valve is fully open (aligned with the

exit pipe)

11. Return Valve to closed (pointing up) position

12. Turn ON main water supply valve

13. Turn ON main 208V switch and boiler toggle

switch (left rear)

14. Re-insert 110V power plugs

15. Watch Glass tube on side of boiler to ensure it

has refilled to acceptable level

16. Turn Autoclave back on

17. If RED Button (low boiler water level) on front

panel is lighted (out), press to reset

18. Resume operations

Blowdown line to

floor drain on other

side of the wall by

Millipore, steam exits

there.

Autoclave: Boiler Blowdown &

Maintenance (from Boiler manual)


Autoclave: Primus, Boiler Plumbing Diagram


Drain/Pressure

and Blowdown

release valve

1/4” Water

Supply line

Water level glass

tube

On/Off Master

switch (access

from rear of unit

Autoclave: Primus, PRI-Pure RO System Diagram 1/2


Autoclave: Primus, PRI-Pure RO System Diagram 2/2


Autoclave: Primus, Pri-Pure RO System


Autoclave: Sterilizer, BRDG, Schematic


Link to Primus Clinical –

Small Size Spec Sheet

.pdf

110v 110v 110v

208V

Power 3 outlets

110V, 15Amp

For Boiler

208V, 67Amp

See Spec shts

Drain and Overflow Line

Primus

Clinical

Model A

Autoclave

B-E. Installation

Jeremy Jamerson

314-680-4248

Link to Primus Dwg

.pdf

Millipore DI

water system

(ref only, not

connected)

208V 3-Phase

Power Switch, On/Up position

Bldg Cold Water

Feed Line

Remove Panel to

access Blow-out

Valve (Weekly)

RO Filters and 100L tank

To Boiler

Water Line Filter

non-boiler use

Bldg Cold

Water Feed

to RO/Boiler

Compressed

Air (not

used)

users.stlcc.edu/Departments/fvbio/Autoclave_BRDG_Primus_ClinicalSpecsheet.pdf

users.stlcc.edu/Departments/fvbio/Autoclave_PrimusA_drawing.pdf

Autoclave: Primus PRI-Pure RO System

(Futuramics Clean Water, www.omahawater.com),

to Boiler and Water Line Filter (added June 2012)


Autoclave: Primus, Water Line-feed Filter (Grainger; Aqua-Pure)


Grainger Item # 2P275

Price (ea.) $124.75

Brand AQUA-PURE Mfr.

Model # AP101T

Valve in Head, Single High,

Size 3/4 In FNPT,

Color Clear,

Length 14 1/4 In, Width 4 9/16 In, Height 14 1/4 In,

Max. Temp. 100 F,

125 Max. Pressure (PSI),

Dia. 4 9/16 In.,

Reduces Dirt And Rust,

Plastic Housing Material,

Cartridges Required 1

Includes 1P753 Cartridge

Water Filter Cartridge,

Material of Construction Melt Blown Polypropylene,

Rating 5 Microns,

Application Rust and Dirt,

Flow 8 GPM,

Height 9 3/4 Inches, Diameter 2 5/8 Inches

Grainger Item # 1P753

Price (pk.) $25.55

Package Qty. 2

Brand AQUA-PURE

Mfr. Model # AP110

Recommended by Jeremy of B&E

4/26/12 for non-boiler related water use

http://www.aquapurefilters.com/store/product/00026.200018/ap101t.html

HotLink to Aqua-Pure Manual .pdf

Users.stlcc.edu/Departments/fvbio/Autoclave_WaterFilter_AP100SeriesManual.pdf

Autoclave: Water Line Feed,

AP101T, Filter Replacement,

every 6mo


Autoclave: Primus, Print Interval Setting


Autoclave: Primus, Loading Thermal Paper


Paper Can be Ordered from Supply

Concepts: Item #: SPR-25347 $2.24

Sparco Co.

Thermal Paper Roll, 2-1/4"x85', 3/PK,

Or OfficeMax:

Item# 20045997 Manf# OM98105

Thermal 2 1/4" x 85' 12rolls/pk $19.99

First : Remove the

Take-up Reel and

SAVE the take-up reel

by pushing it out of

the spool.

Autoclave: Primus, Loading Printer Take-up Spool


Recommended Method (BobM)

1. Place empty spool in the area of the

hubs

2. Use a screwdriver to open/expand

the width of the open slot to make it

easier to snap over the hub ends

3. When empty spool is in place, fold

paper into a “V”, insert into spool

slot

4. Press FEED to take up some paper

on spool.

Autoclave: Thermometer, Temp Max after cycle


Fisher Sci: CAT # 15-114-10 Each for $66.95

For use in autoclaves without built-in temperature recording devices

Has constricted capillary to hold temperature after heat is reduced so you can monitor maximum

temperature reached during sterilization.

Thermometer, Autoclave; Fisherbrand; Length: 200mm; Total immersion; Sun division: 0.5°C; Range:

85° to 135°C; Contains mercury

Autoclave: Temperature Loggers (example)


Omega.com

Autoclave Temperature Data Logger

OM-CP-HITEMP140 , Approx $499

Specifications

Temperature Sensor: 100Ω Platinum RTD

Temperature Range (Body): -40 to 140ºC (-40 to 284ºF)

Temperature Measurement Range (Probe): -200 to 260ºC (-328

to 500ºF)

Temperature Resolution: 0.01ºC (0.02ºF)

Calibrated Accuracy: ±0.1ºC (±0.18ºF) [-20 to 140ºC (-4 to

284ºF)]

Start Modes:

• Software programmable immediate start

• Delay start up to eighteen months in advance

Real Time Recording: May be used with PC to monitor and

record data in real time

Memory: 32,700 readings

Reading Rate: One second up to once every 24 hours

Battery Type: 3.6V high-temperature lithium battery (included)

Battery Life: 1 year typical [1 minute reading rate at 25ºC (77ºF)]

Calibration: Digital calibration through software

Calibration Date: Automatically recorded within device

Data Format: Date and time stamped ºC, ºF, K, ºR

Time Accuracy: ±1 minute/month at 20 to 30ºC (68 to 86ºF)

(RS-232 cable not in use)

Computer Interface: OM-CP-IFC400 USB docking station

required 125,000 baud

Software: XP SP3/Vista/Windows® 7 (32- and 64-bit)

Operating Environment: -40 to 140ºC (-40 to 284ºF), 0 to 100%

RH

Dimensions

Body: 67 H x 25 mm D (2.7 x 0.9")

Probe: 50 L x 3.8 to 3.2 mm transitional dia (2.0 x 0.188 to

0.125")

Weight: 120 g (4.2 oz)

Material: 316 stainless steel

OM-CP-HITEMP140 3 Weeks $499.00

Autoclave temperature data logger qty.

OM-CP-HITEMP140-CERT $559.00

Autoclave temperature data logger and NIST certificate

qty.

OM-CP-IFC400 $120.00

Docking station with USB cable, software and manual

qty.

OM-CP-BAT110 $50.00

Replacement 3.6 V high-temperature lithium battery

Autoclave FV ; General Information


Description: Tuttnauer 5596 SP -1 Sterilizer Dimensions: 43” W x 56” D x 75” H (External Stainless walls with enclosed boiler and pump. ) Requires: DI and standard cold water input and 1.5” exhaust/vapor line. Clearances: 500mm on sides and rear. Front should have clear access for trays on carts. • Chamber size is 15" dia x 29.5" deep.

Chamber Dimensions WxDxH mm (inc.): 508 x 508 x 970

Chamber Volume (Liters): 250

Total Weight with all components 1000 KG

• High speed, high vacuum, high capacity, hospital grade sterilizer with FDA cleared flash cycle • A built in vacuum pump and steam generator allows for increased load size and dramatically reduces total cycle time. • The closed door drying system provides a faster and more thorough drying cycle. Seven automatic cycles are available. • Five sterilization cycles and two test cycles. • Designed for sterilization of medical and surgical materials such as packed and wrapped solids and instruments.

Link to B-E Hospital Equip Co, Inc .

STL sales/service

Wally Rodemacher tech Spec.

314.241.0855

http://www.behospital.com/

• Chamber Size: 20x20x38 with Manual Hinged Door

• Chamber Volume: 250 Liters

• External Dimensions: 30”W x 56”D x 75”H

• Carbon Steel Electric Steam Generator (Integral)

• Free-Standing with Stainless Steel Cabinet Enclosure

• Standard Cycles: (4)Gravity, (4)Pre-Vac, (4)Liquid and (2)Test Cycles

• Utilities required (by others): Water (approx. 50Mohm), 3-phase electric, Compressed air, Floor Drain

• Designed for sterilization of porous and non-porous material, liquids, glassware etc.

• Warranty: 1 year parts and labor, 15 years on the pressure vessel

• Accessories: Rack with two shelves

Options not included:

• Integral air compressor (if house air is not available)

• Auto- Vertical Sliding Door (Model 5596 ECP-V)

Autoclave: FV-Tuttnauer Model 5596


Link to General Notes for

Architects/Contractors .pdf

Link to Installation and Clearance

Drawing .pdf

Autoclave: General Information from: David Morganstern Sales Manager tuttnauer USA

25 Power Drive Hauppauge, NY 11788Ph: 800-624-5836 x112 Fax: 631-737-1034 E-mail:

[email protected]

users.stlcc.edu/Departments/fvbio/BRDG_Autoclave_Manf_Notes.pdf

users.stlcc.edu/Departments/fvbio/BRDG_Autoclave_Installation.pdf

Autclave FV; Inside Panel Views; Tuttnauer 5596


Air Compressor and tank; out for

service goes into lower bay

Boiler for Steam/Pressure

RODI Water storage from

Millipore system

Autoclave : Tuttnauer Basic Operating Instructions

1. Open the main steam inlet valve and watch the jacket pressure gauge on the front

panel. When the jacket pressure reaches 2.3 bars, the sterilization cycle can be

started. (condensate may exit through the jacket steam trap during this period)

2. Open the door by turning the control wheel handle counter clockwise to release the

locking arms.

3. Load the material to be sterilized per the guidelines in this protocol

4. Close the door by pushing towards the frame and turning the wheel handle

clockwise until the “Door Closed” lamp is lit.

5. On the control panel, chose the sterilization program according to the materials

loaded. The light for the program should be on with parameters displayed.

6. Press the START key to begin the cycle. Lights on the bottom indicate the stage of

the cycles in progress. – Air is purged from the chamber at the beginning of the sterilization cycle.

– Steam under pressure is introduced into the chamber and the temperature rises to the desired level.

– The autoclave is maintained under pressure at a high temperature for a predetermined time.

– Steam is then exhausted from the chamber at the end of the cycle.

7. A buzzer will sound and the START light will turn off at the end of the cycle. The

printer will print a summary of the cycle.

8. Open the door by turning the wheel handle counter clockwise.

9. Remove sterilized materials carefully using orange or heat resistant gloves


Autoclave : Tuttnauer Safety Features and Audible Alarms 1. Steel bar Door Locks

2. Safety Vent/Vent Valve

• Safety valve – opens as back-up protection to reduce chamber pressure in the

event of exceeding 262 kPa

• Vent Valve – needed if chamber pressure should exceed 241kPa, the vent

valve will open and the P-2 alarm will display.

3. Always use “HOT GLOVES” (orange) when removing items

from the Autoclave

1. Overheat Protection – chamber temperature is set so as not to exceed 149 C and has

additional overheat protection if temperature reaches 180 C.

2. Electrical power interruption – in case of an electrical power failure during the

sterilization cycle, pressure in the chamber is automatically vented to the atmosphere.

3. Audible Signals

– Each time a switch is depressed one beep occurs (except when depressing the four arrows).

– Five beeps indicates sterilization cycle is complete.

– Five beeps indicates drying cycle is complete.

– Six beeps operational error or alarm.

– Continuous beeps – door is open during fill cycle.


Autoclave : Tuttenaeur

Control and Monitoring Keys

• Unwrapped Instruments (without drying stage)

• Wrapped Instrumentss (with drying stage)

• Liquids; pressing one of the program keys

determines the stages to be run

• Vacuum test/Bowie Dick test; press once for

Vacuum, twice for Bowie test

• TEMP; shows sterilization temperature. Press

UP/DN keys to permanently change this value

from the nominal setting.

• STE Time; shows sterilization time. Use

UP/DN to permanently alter this setting.

• DRY Time; shows drying time. Alter with

UP/DN keys.

• Clock ; Press to change settings with UP/DN

keys.

• START; begins selected sterilization program.

Will not start if door in not secured.

• STOP; Only key that can be used to stop

operations once a program cycle has begun.

PROGRAM CYCLE or STATUS


Autoclave: Tuttnauer Sterilizing Media in Flask; Setup


1. Make sure pressure Jacket

pressure is at or above 18 psi

2: Select Liquids on control

panel

(prepared from: E. Lyons 10/22/08)

Autoclave: Tuttnauer Sterilizing Media in Flask; Run Settings, Door

operations


3. Check to make sure that the

autoclave is set to run for 20 minutes

at 250F. (The photo shows that this is

program 12, and the temperature and

time are set correctly.)

4. Open the autoclave door by turning

counter clockwise. Place the flasks in

the autoclave with some room

between them. Close the door tightly

with clockwise motion.

Close Door:

Clockwise

CW

Open Door:

Counter

Clockwise

CCW


Autoclave: Tuttnauer Sterilizing Media in Flask ; Start and Run

Verify


5. Press the START button on

the control panel.

6. Check to make sure that the

autoclave has functioned properly

during the heating cycle by checking

the recording tape for errors. (This

tape can be copied for inclusion in

your lab notebook.)


DATE 4/13/2009 TIME 4:36:07

PROG Liqud1

TEMP 250F TIME 20 min DRY 00 min EXH MODE 4 END TEMP 203 MN TEMP PRESS V01 231F 15P V02 201F 15I S04 250 17P S05 252 17P S06 252 17P S07 252 17P S08 252 17P S09 252 17P …. … … S23 252 17P S24 251 16P E25 247 13P E28 229 04P E31 218 00P E34 215 00P E37 210 00P E40 210 01I E43 210 01I E46 210 01I E49 204 01I E49 204 01I O.K. Operator Load NO 5 Autoclave No. 12

Autoclave: Tuttnauer Run Print Tape

Examples

PROGS: Liqud1, UnWrp1, UNWrp3


DATE 4/13/2009 TIME 4:36:07

PROG UnWrp1 TEMP 250F TIME 20 min DRY 10Min EXH MODE 1 MN TEMP PRESS V01 231F 15P V03 191 15I S04 250 17P S05 252 17P S06 252 17P S07 253 17P S08 252 16P …. … … S18 255 18P E20 237 03P D20 183 23I D22 197 22I D28 168 23I D30 162 22I O.K. Operator Load NO 7 Autoclave No. 12

DATE 4/13/2009 TIME 4:36:07

PROG UnWrp3 TEMP 270F TIME 05 Min DRY 00Min EXH MODE 1 MN TEMP PRESS F08 079F 01P F09 079F 01P Operator Load NO. 2 Autoclave No. 12

Autoclave: Sterilizing Media in Flask ; Removing and Cooling


7a. When the autoclave is finished and the inside

pressure has dissipated, remove your flask of media

using orange hot gloves.

7b. Swirl the media gently because the agar may

settle to the bottom of the flask during autoclaving.

8a. Place the media into a water bath set at 55-60C,

which is the temperature it should cool to for pouring

the plates. (Note: If the solution is not placed in a water

bath, cool until you can just hold your bare hands on

the bottom of the flask.

8b. If it begins to solidify, it will have to be re-melted by

autoclaving again for about 5 minutes.


Autoclave: Sterilization Process and Verification

• Sterilization – the highest level of disinfection; the killing of all living

organisms on a surface

• Testing whether sterilization has occurred during autoclaving is done

using spores (dormant microorganisms)

– Spore strips are dried pieces of paper to which large number of

pathogenic , heat-resistant Bacillus thermopholus spores are

adhered.

– After autoclaving the strips are transferred to growth medium, if

successful autoclaving, then the spores will not grow.


Autoclaves: Cycles, Gravity vs. Vacuum


Gravity Cycles: Displacement Autoclaves – The Old Workhorse

Traditional models rely upon gravity to displace the air from the sterilizing chamber. Steam is much lighter than air and, as it is

injected into the pressure chamber, it forces air downwards, trapping it in a sink. A system of valves and taps bleed out this excess

air, allowing the steam to sterilize the contents of the chamber.

These autoclaves are the cheapest option and they are very easy to use. As a result, they are common in kitchens and manicurists,

where they are more than adequate. However, for sterilizing porous or wrapped materials, displacement autoclaves are not efficient

enough. Hot air may be trapped inside, allowing pockets of microbes and other pathogens to evade the sterilization cycle.

Vacuum Autoclaves – The Safer Solution to Sterilization

For complex sterilization, or in areas where complete sterility is necessary, a vacuum autoclave is the superior choice. Vacuum

autoclaves evacuate the air, with a vacuum, and steam is then injected into the chamber. This allows the steam to penetrate porous

materials, wiping out any pockets of pathogens in hard-to-reach areas.

After this steam sterilization stage, the pump again evacuates the chamber, drying the autoclave contents thoroughly. This added

stage prevents germs and prions being retransmitted by trapped moisture. Vacuum autoclaves are the preferred option in medical

institutions, as a more effective alternative to the simpler displacement autoclaves.

Many hospitals use disposable medical equipment, to prevent the transmission of CJD variants, but the contaminated waste is still

autoclaved before landfill. Autoclaving is a more environmentally friendly method than traditional incineration, which ejects toxic

gases into the atmosphere.

Autoclave; Tuttnauer 5596 SP Series



Autoclave : Tuttnauer Utilities Consumption

• 16”x16”x28”

20”x20”x38”

• H44 • D

• W

• H

• D

• W

• H

• D

H55 Series

Autoclave/Sterilizer: BRDG Proposal, STERIS/AMSCO-Lab 250


The Amsco Lab 250 sterilizer was designed with the needs and

the budget of the laboratory research market in mind. The lab

series sterilizer provides the same time-proven, safe, reliable

chamber and stand assembly as the Century® sterilizer that has

been offered to the healthcare and life sciences markets since

1992.

The Amsco Lab 250 features an Allen-Bradley MicroLogix"

control and a full-color operator interface. The unit has a

chamber size of 20 x 20 x 38 inches (508 x 508 x 965 mm) and

is equipped with gravity and liquid type cycles as standard.

Optional prevacuum and isothermal cycles are also available if

required. A manually operated vertical sliding door is combined

with the non-lubricated door seal for reliability and ease of use.

The drain line piping package meets or exceeds the maximum

water temperature requirements of the industry - 60ºC (140ºF).

Link to AMSCO Lab 250 Spec Sheets…. pdf

Link to AMSCO Lab 250 Brochure…. pdf

Link to AMSCO Lab 250 Tech Sheets…. pdf

users.stlcc.edu/Departments/fvbio/Autoclave_Sterilizer_Lab250_cutsheet.pdf

users.stlcc.edu/Departments/fvbio/Autoclave_Sterilizers_Lab 250_brochure.pdf

users.stlcc.edu/Departments/fvbio/Autoclave_Sterilizer_AMSCO250_Techdoc.pdf

Autoclave/Sterilizer: BRDG Proposal,

AMSCO Lab 250 Spec Sheet


Sterilizer: Bacti-Cinerator


• Sterilizes loops and needles safely and conveniently.

• Prevents infectious spatter and/or cross contamination.

• Safeguard laboratory personnel -no open flame -no

hazardous gas.

• Ideal for use in anaerobic chambers.

• Core Tube does not contain asbestos

On/off toggle switch

Heat element “on” indicator

Oxford® Bacti-Cinerator® IV (Cole-Palmer)

EW-01850-26

Replacement Heating Element for 01850-22 (120V)

Sterilizer; Bacti Cinerator- Features, Safety

• Allow 6-10 minutes for the Bacti-Cinerator to reach an

optimum sterilizing temperature of 815°C (1500°F) throughout the interior of the core.

• 5-7 seconds of exposure sterilizes loops, needles and culture tubes. Unit is not intended

for use with scalpels, forceps or sharp instruments.

• Use only insulated loop holders when inserting loops into the heater element.

• Suitable for anaerobic procedures in chambers and hoods.

• Yellow Light indicates that the heating element is ON. Note, the Core element may

remain very hot for 30 minutes after it is turned off. Allow 1 hour of cooling before

cleaning or storage.

• Avoid any contact with the heat shield or end caps while the Sterilizer is on.

• Do not immerse the sterilizer in water or other cleaning solutions.

• Periodically inspect the element for cracks (yellow-orange fissures) and wear.


Core will glow red-hot

when approx 815C temp has

been reached

Extremely Hot surface. Do not

touch or allow other items to

come in contact

Do not wrap power cord around

Heat element for storage.

Sterilizer: Bead


Effectively sterilize small, solid metal and glass implements in

seconds

• Heated glass beads destroy all microbes and spores

• Low power consumption allows for all day operation

• Safe to use—no gases, fumes, open flames or harsh liquids


Bead Sterilizer: Setup and Operating Protocol

1. Sterilizer Usage:

• Remove the metal cover cap before turning on the unit

• Allow 15 minutes to reach 240C before use

• Unit can be left on for further use

• Turn off at the end of the day

• Replace metal cap when cooled to prevent contamination

2. Caution: Do not touch beads or sides of container when in use.

3. Small Dish:

• Use for small metal objects

• Cover dish with metal cap for 3 minutes maximum

• Remove dish and cap when finished

4. Glass beads are heated to 250°C to effectively destroy all microbes and spores.

Suitable for sterilizing scissors, forceps, scalpels, inoculating loops and needles.

5. Power consumption is low allowing for all day operation.

6. Dial thermometer on front of sterilizer ensures proper heating and back-up safety

thermostat prevents over-heating.

Sterilizer/Heat Source- Micro Burner Butane


This small butane burner uses clean burning butane and features a

trigger ignition, eliminating the need for wicks, matches or a central

gas line. Avg use rate 2oz/hr, refill about every 45min

Sterilizer/Heat Source- Micro Burner Butane


autoclave (primus)

Documents