DescriptionThe Atlas® Human Hematology/Immunology Array includes 406 human cDNAs, nine housekeeping control cDNAs, and negative controls immobilized in duplicate dots on a nylon membrane. Two identical arrays are included, along with reagents for synthesizing radioactively labeled cDNA probes. A transparent Orientation Grid is also provided to allow identification of cDNA's corresponding to the positive hybridization signals. See the enclosed CD-ROM for functional classes and complete gene representation on this array.

package contents

Box 1: 2 Atlas Human Hematology/Immunology

(10 ng of cDNA/dot)12 µl Hematology/Immunology CDS Primer Mix

1 Blank Nylon Membrane(s)1 Orientation Grid(s) (PT3140-2)

Box 2:120 µl 10X dNTP Mix (for dATP label)

(5 mM each dCTP, dGTP, dTTP)600 µl 5X Reaction Buffer 15 µl MMLV Reverse Transcriptase

(100 units/ µl)120 µl DTT (100 mM) 60 µl 10X Termination Mix 2.5 µg Human Placenta Control Poly A+ RNA

(0.5 µg/µl) 65 µl Human C0t-1 DNA 1.2 ml Deionized H2O

Box 3:100 ml ExpressHyb™ Hybridization Solution 1 Atlas NucleoSpin® Extraction Kit

otherUser Manual (PT3140-1)CD-ROM (PT3593-CD)

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proDUct: atlas® human hematology/immunology array

cataLog no. 634507

Lot nUMBerSpecified on product label

storage conDitions Store Atlas Arrays at –20°C sealed in a plastic bag away from light.Store Express Hyb™ Hybridization Solution and Atlas NucleoSpin Extraction Kit at room temperature. Before using hybridization solution, dissolve any precipitate with gentle warming. Store Control Poly A+ RNA at –70°CStore all other reagents at –20°C

sheLF LiFe1 year from date of receipt under proper storage conditions.

shipping conDitionsBoxes 1 & 2: Blue ice (4°C) or Dry ice

(–70°C)Box 3: Room temperature

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APPROVED BY: _________________________

(PA2Y194)

For research Use onLY

QUaLitY controL Data

See back of page.

QUaLitY controL Data

1. The size and identity of all cDNAs were verified by PCR amplification, agarose/EtBr gel electrophoresis and sequencing.

2. The Control Poly A+ RNA was electrophoresed on a formaldehyde/agarose EtBr gel. The pattern observed was that expected for human placenta poly A+ RNA.

3. A biotinylated cDNA probe was synthesized using the control poly A+ RNA according to the Atlas SpotLight™ Labeling Kit (Cat No. 634803) User Manual or a radioactively labeled probe was synthesized using the Control Poly A+ RNA according to the Atlas Array User Manual. This probe was hybridized to the array, and the expected hybridization pattern was observed.

Atlas® Human Hematology/Immunology Array Cat No. 634507

PA2Y194 www.clontech.com Clontech Laboratories, Inc.

notice to purchaser

Clontech products are to be used for research purposes only. They may not to be used for any other purpose, including, but not limited to, use in drugs, in vitro diagnostic purposes, therapeutics, or in humans. Clontech products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of Clontech Laboratories, Inc.

The Atlas® Array products sold by Clontech® are for research purposes only. Certain DNA sequences included on the Atlas Arrays may be covered by U.S. patents. Presently, it is not clear under U.S. laws whether commercial users must obtain licenses from the owners of the rights to these U.S. patents before using Atlas Arrays.

These products and the sequences of the polynucleotides thereon are intended to be used for the purchaser’s own internal research purposes only and may not be used for drug diagnostic purposes or human use.

DISCLAIMER OF RIGHTS

No license is conveyed with the purchase of this product under U.S. Patents Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056 and 6,171,785, and corresponding patent claims outside the U.S., relating to the 5’ nuclease and dsDNA-binding dye processes. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Clontech, the Clontech logo and all other trademarks are the property of Clontech Laboratories, Inc. Clontech is a Takara Bio Company. ©2006