• ATB: MAKE A NEW LOGBOOK ENTRY “PROTEIN NETWORK PRACTICE 2”

• GET A SHEET FROM THE FRONT COUNTER—1 PER PERSON

• PLANNER: DESIGN YOUR OWN NETWORK DUE FRIDAY

• CHECKUP/QUIZ ON FRIDAY• LOGBOOK CHECK AT END OF CLASS TODAY• IF YOU MISSED THIS MONDAY’S CHECKUP—

MAKE IT UP! IT TURNS INTO A ZERO AFTER FRIDAY

Lactose

Glucose

Lactase

Galactose

Key:

Metabolites

Enzyme reactions

Labrador (dog) pigmentation

Tyrosine Dopaquinone

Brown pigment

Black pigment

Yellow pigmenttyrosinase

TRP-2

TRP-1Boxes = metabolitesArrows = enzymes that change the metabolites

Phenylalanine

Homogentisicacid

Tyrosine

Tyramine

Thyroxine

Hydroxy-phenylpyruvate

Dopa

Dopamine

NoradrenalineAdrenaline

Dopaquinone Melanin

Phenylpyruvate

Maleyl-acetoacetate

Prephenate

1

2 4

35

6

2-amino-4-hydroxypterdine

(BLUE)

tetrahydroiopterin

biopterin(BLUE)

drosopterin(ORANGE)

sepiapterin(YELLOW)

isosepiapterin(YELLOW)

xanthopterin(GREEN-BLUE)

isoxanthopterin(VIOLET-BLUE)

Molecule A(colorless)

PTERIDINE PIGMENTS OMMOCHROMEPIGMENTS

Molecule B(colorless)

ommochrome pigment(BROWN)

31

5

4

6

2 9

7

8 10

In order to see pigments, they must be transported into specific areas of the eye cells. All the ommochrome and pteridine pigments use the same transport proteins (labeled as protein #10 on the network)

Color of fly is result of mixture of all pigments in network

Drosophila Eye Color Pigment Network

Wild Type Brown

Scarlet White

2-amino-4-hydroxypterdine

(BLUE)

tetrahydroiopterin

biopterin(BLUE)

drosopterin(ORANGE)

sepiapterin(YELLOW)

isosepiapterin(YELLOW)

xanthopterin(GREEN-BLUE)

isoxanthopterin(VIOLET-BLUE)

Molecule A(colorless)

PTERIDINE PIGMENTS OMMOCHROMEPIGMENTS

Molecule B(colorless)

ommochrome pigment(BROWN)

31

5

4

6

2 9

7

8 10

In order to see pigments, they must be transported into specific areas of the eye cells. All the ommochrome and pteridine pigments use the same transport proteins (labeled as protein #10 on the network)

Color of fly is result of mixture of all pigments in network

Drosophila Eye Color Pigment Network

Serotonin

• Serotonin is a molecule called a neurotransmitter used by cells in the brain to communicate

• Part of its function is to regulate emotions• Abnormally low amounts of serotonin in the

brain can cause depression

How is serotonin produced, and how can we use our understanding of it to treat depression?

Some enzymes involved in serotonin synthesisEnzyme: AAADSubstrate: 5-HTPProduct: serotonin

Enzyme: TPHSubstrate: tryptophanProduct: 5-HTP

Enzyme: MAOSubstrate: serotoninProduct: 5-HIAA

5-HIAA

5-HTP

Draw the enzyme pathway used to build serotonin

5-HIAA

5-HTP

TPH

AAAD

MAO

Tryptophan5-HIAA

5-HTPSerotonin