associate professor and head department of sericulture ... · associate professor and head...
TRANSCRIPT
Translation
Dr. Mahesha H BAssociate Professor and Head
Department of Sericulture
Yuvaraja’s College
University of Mysore, Mysuru, India
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Translation is a process where proteins are made by the ribosomes on the mRNA strand.
Or
The process in the ribosomes of a cell by which a strand of messenger RNA directs the assembly strand of messenger RNA directs the assembly of a sequence of amino acids to make a protein.
DNA ���� RNA ���� PROTEIN
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Site of protein synthesis in eukaryotes and prokaryotes
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Prokaryotic cell
Eukaryotic cell
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Ribosome
0.9X106
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5S rRNA with 120 nucleotides23S rRNA with 2904 nucleotides31 different proteins L1,L2,L3,.....
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Activation of amino acids
Transfer of amino acid to tRNA (charging of tRNA)
Initiation of synthesis
Steps in Translation
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Initiation of synthesis
Elongation of polypeptide chain
Termination of synthesis
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Role of RNAs in translation
rRNA is integral part of
ribosome
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tRNA transports amino acids mRNA carries genetic
information
Activation of amino acids & Charging of tRNA
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Initiation of translation
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IF 3 Mediates – Shine-Dalgarno sequence in 16 s rRNA
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Initiation - over
L and S proteins forms A site and P site
Initiation - over
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Elongation - starts
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Pepidyl transfarase centre/ active centre created b y L proteinswww.hbmahesh.weebly.com
Peptidyl transferase centre/ active centre
Formation by L proteins of large sub unit
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EF-G
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ELONGATION ELONGATION ELONGATION ELONGATION ---- OVEROVEROVEROVER
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RFRFRFRF----3+GTP3+GTP3+GTP3+GTP
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TERMINATION TERMINATION TERMINATION TERMINATION ---- OVEROVEROVEROVER
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→ → →→
+IF1,IF2,IF3
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ACKNOWLEDGEMENTSACKNOWLEDGEMENTSACKNOWLEDGEMENTSACKNOWLEDGEMENTS
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