assessment of allelopathic potential of different ... assessment of allelopathic potential of...

Download ASSESSMENT OF ALLELOPATHIC POTENTIAL OF DIFFERENT ... ASSESSMENT OF ALLELOPATHIC POTENTIAL OF DIFFERENT

Post on 10-May-2020

0 views

Category:

Documents

0 download

Embed Size (px)

TRANSCRIPT

  • ASSESSMENT OF ALLELOPATHIC POTENTIAL OF

    DIFFERENT WEEDS ON GERMINATION AND

    EARLY GROWTH OF AROMATIC RICE

    A Thesis

    Submitted to

    Bangladesh Agricultural University, Mymensingh

    In partial Fulfillment of the Requirements

    for the Degree of

    Master of Science

    in

    Agricultural Chemistry

    By

    Monira Yasmin

    Roll No.: 10Ag.Chem JD-05M

    Registration No.: 32087

    Session: 2005-06

    Department of Agricultural Chemistry

    Bangladesh Agricultural University

    Mymensingh

    November, 2011

  • ASSESSMENT OF ALLELOPATHIC POTENTIAL OF

    DIFFERENT WEEDS ON GERMINATION AND

    EARLY GROWTH OF AROMATIC RICE

    A Thesis

    Submitted to

    Bangladesh Agricultural University, Mymensingh

    In partial Fulfillment of the Requirements

    for the Degree of

    Master of Science

    in

    Agricultural Chemistry

    By

    Monira Yasmin

    Approved as to style and content by

    (Prof. Dr. Md. Mokhlesur Rahman)

    Supervisor

    (Prof. Dr. Hari Pada Seal)

    Co-supervisor

    (Dr. Md. Zakir Hossen)

    Chairman, BOS and Head

    Department of Agricultural Chemistry

    November, 2011

  • ACKNOWLEDGEMENT

    All praises are due to the Almighty Allah Whose divine blessing enabled the researcher to complete

    her research work and prepare the thesis successfully for the degree of MS in Agricultural

    Chemistry.

    The author likes to express her gratefulness and indebtedness to her respected supervisor Prof. Dr.

    Md. Mokhlesur Rahman, Department of Agricultural Chemistry, Bangladesh Agricultural

    University, Mymensingh for his kind advice, valuable guidance and constructive suggestions to

    carry out the research work and preparation of this thesis.

    The author acknowledges her heartiest gratification and sincere appreciation to her honorable co-

    supervisor, Prof. Dr. Hari Pada Seal, Department of Agricultural Chemistry, Bangladesh

    Agricultural University, Mymensingh for his sincere advice, planning, upgrading the quality of

    the research work and for help in preparing the manuscript of this thesis.

    The author humbly desires to acknowledge her sincere gratitude to Prof. Dr. M. Wahid-U-

    Zzaman, Prof. Kartik Chandra Saha, Prof. Dr. Md. Akhter Hossain Chowdhury, Dr. Md. Zakir

    Hossen, Associate Professor and Head, Dr. Quazi Forhad Kadir, Associate Professor, Dr. Atiqur

    Rahman, Assistant Professor, Dr. Razia Sultana, Assistant Professor, Dr. K. M. Mohiuddin,

    Assistant Professor, Mrs. Mousumi Akter, Lecturer, Mosammat Hasina Akter, Lecturer and Mr.

    Md. Amdad Ali, Lecturer, Department of Agricultural Chemistry for their valuable teaching,

    inspiration and encouragement during the whole course of this study.

    The author would express her special thanks to the staff members, Department of Agricultural

    Chemistry, Bangladesh Agricultural University, Mymensingh for extending all laboratory

    facilities for completing research work.

    The author is pleased to express her cordial thanks to all of her friends, classmates and well

    wishers for their immense inspiration, and kind co-operation during conducting experiment and

    writing of the thesis.

    The author would like to express heartfelt indebtedness to her respected parents, sisters, friends

    and all other relatives for their never ending prayer, encouragement, sacrifice and dedicated efforts

    to educate me to this level which can never be forgotten.

    The Author

  • ABSTRACT

    A laboratory experiment was carried out at the Department of Agricultural

    Chemistry, Bangladesh Agricultural University, Mymensingh to investigate the

    allelopathic potential of different weeds on aromatic rice cv. BRRI dhan50. Six

    weed species viz., joina (Eimbristylis miliacea), mutha (Cyperus rotundus), khude

    shama (Echinochola colonum), sobuj nakful (Cyperus difformis), foskabegun

    (Physalis heterophylla) and chapra (Elusine indica) were used in the experiment

    to observe their allelopathic effects on seed germination, root length, shoot length

    and dry matter production of aromatic rice. Unboiled and boiled weed extracts

    reduced the germination and primary growth of aromatic rice. The ranking of

    weed species in respect of inhibitory effect on seed germination were Echinochola

    colonum > Cyperus difformis > Cyperus rotundus > Elusine indica > Eimbristylis

    miliacea > Physalis heterophylla. Khude shama (Echinochola colonum) and sobuj

    nakful (Cyperus difformis) had strong detrimental effects on the early growth of

    aromatic rice. The relative effect of different weed plant parts (stem, root, leaf and

    whole plant) and their concentrations (5, 10 and 25% w/v) were also studied. The

    extracts irrespective of plant parts of all weeds inhibited germination and early

    growth of aromatic rice. The relative efficiency of reduction of germination and

    primary growth by different weed extracts was in the order of stem > root > leaf >

    whole plant. The reduction of germination and primary growth of aromatic rice

    increased with the increasing extract concentrations. Three unknown compounds

    were identified by the TLC technique in extracts of khude shama (Echinochola

    colonum) and sobuj nakful (Cyperus difformis). The experimental results proved

    that it was important to exclude two allelopathic weeds namely khude shama

    (Echinochola colomum) and sobuj nakful (Cyperus difformis) in the aromatic rice

    field. It is, therefore, concluded that these two weeds should be avoided from the

    aromatic rice field during land preparation.

  • CONTENTS

    Page No.

    ACKNOWLEDGEMENT iv

    ABSTRACT v

    CONTENTS vi

    LIST OF TABLES viii

    LIST OF FIGURES ix

    LIST APPENDICES x

    Chapter 1 INTRODUCTION 1

    Chapter 2 REVIEW OF LITERATURE 4

    2.1 Effect of aqueous extract of different weeds on

    germination and primary growth of crops

    4

    2.2 Effect of different plant parts of weeds on

    germination and primary growth of crops

    9

    2.3 Effect of different concentrations of weed extract on

    germination and primary growth of crops

    13

    Chapter 3 MATERIALS AND METHODS 18

    3.1 Test crop 18

    3.2 Test weed species 18

    3.3 Experimental treatment 18

    3.4 Collection of weeds and preparation of weed

    extracts

    19

    3.5 Experimental procedure 19

    3.6 Parameters 20

    3.7 Experimental data 20

    3.7.1 Seed germination 20

    3.7.2 Germination time of rice seeds 20

    3.7.3 Root and shoot lengths of seedlings 20

    3.7.4 Fresh weights of root and shoot of seedlings 20

    3.7. 5 Dry weights of root and shoot of seedlings 21

    3.8 Identification of chemical compounds 21

    3.9 Calculation of Rf values 22

    3.10 Data analysis 22

  • CONTENTS (Contd.)

    TITLE Page No.

    Chapter 4 RESULTS AND DISCUSSION 23

    4.1 Allelopathic effect of different weed species on

    aromatic rice

    23

    4.1.1 Effect of unboiled and boiled weed extracts on

    seed germination

    23

    4.1.2 Effect of unboiled and boiled weed extracts on

    primary growth of seedlings

    25

    4.1.2.1 Root and shoot lengths 25

    4.1.2.2 Fresh and dry weights of root 30

    4.1.2.3 Fresh and dry weights of shoot 30

    4.2 Allelopathic effect of different weed plant parts of on

    rice

    31

    4.2.1 Effect of different weed plant parts on

    germination

    31

    4.2.2 Effect of different weed plant parts on primary

    growth

    32

    4.2.2.1 Root and shoot lengths 32

    4.2.2.2 Fresh and dry weights of root 33

    4.2.2.3 Fresh and dry weights of shoot 33

    4.3 Allelopathic effect of extracts of different weed

    plant parts at different concentrations

    36

    4.3.1 Interaction effect of weed plant parts and

    extract concentrations on germination

    36

    4.3.2 Interaction effect of weed plant parts and

    extract concentrations on initial growth

    36

    4.4 TLC Techniques for extraction of khude shama

    (Echinochola colonum) and sobuj nakful (Cyperus

    difformis)

    40

    Chapter 5 SUMMARY AND CONCLUSION 43

    REFERENCES 46

    APPENDICES 51

  • LIST OF TABLES

    Table TITLE Page No.

    4.1 Effect of unboiled weed extracts on germination and

    early growth of aromatic rice cv. BRRI dhan50

    (Banglamoti)

    26

    4.2 Effect of boiled weed extracts on germination and early

    growth of aromatic rice cv. BRRI dhan50 (Banglamoti)

    27

    4.3 Allelopathic effect of khude shama extract from

    different plant parts on germination and early growth of

    aromatic rice cv. BRRI dhan50 (Banglamoti)

    34

    4.4 Allelopathic effect of sobuj nakful extract from different

    plant parts on germi

Recommended

View more >