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Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport
in Bedrock Well Columnin Bedrock Well Columnin Bedrock Well Columnin Bedrock Well Column
Eric J. Raes, P.E., LSRP
Engineering and Land Planning Engineering and Land Planning
Dora Ogles, Brett Baldwin, Kate Clark & Anita Biernacki
Microbial Insights Inc.
Kerry Sublette, Ph.D., Kate Key
University of Tulsa
We We We We knowknowknowknow
That Hydrologist Like to
Draw Arrows
We We We We knowknowknowknow
That Chemist Like using
Colors
But what do we know about microbial But what do we know about microbial But what do we know about microbial But what do we know about microbial
migration?migration?migration?migration?
Let’s ask a friend.
But what do we know about microbial But what do we know about microbial But what do we know about microbial But what do we know about microbial
migration?migration?migration?migration?
Let’s ask a friend.
Microbial Release and Transport StudyMicrobial Release and Transport Study
0.100.120.140.160.180.20
met
er/d
ay
0.000.020.040.060.080.10
0.5 - 1 mm 0.25 - 0.5 mm 0.05 - 0.25 mm
met
er/d
ay
Sand Size Range
Pilot Study Results - IW-1 and Piezometers
PZ-4
IW-1
PZ-3PZ-2 PZ-1
Radius of Influence?Radius of Influence?
0.6016:30 05:42 18:54 08:06 21:18 10:30
-0 .100.080.260.440.620.80
DO
(mg
/L)
16:30 05:42 18:54 08:06 21:18 10:300.5100.5960.6820.7680.8540.940
Sp
Co
n(m
S/c
m)
16:30 05:42 18:54 08:06 21:18 10:3015.2015.3815.5615.7415.9216.10
Te
mp
(C)
WP-1.DAT
16:30 05:42 18:54 08:06 21:18 10:30-444.0-330.6-217.2-103.8
9.6123.0
OR
P(m
V)
16:30 05:42 18:54 08:06 21:18 10:305.806.046.286.526.767.00
pH
16:30 05:42 18:54 08:06 21:18 10:30
0.600.740.881.021.161.30 D
ep
th(m
)
DateTim e(M/D/Y )
12/07/11 12/14/11 12/20/11 12/27/11 01/02/12 01/09/12
Star Pupils
••
GW Flow
Anaerobic Bioreactors, the same but different
100
1,000
10,000
100,000
8
10
12
14
16
(Cel
ls/m
L)
So
lven
t C
on
cen
trat
ion
s(u
g/L
)
MW-1 Solvent Concentrations vs. Time
0
1
10
0
2
4
6
(Cel
ls/m
L)
So
lven
t C
on
cen
trat
ion
s
Date
Tetrachloroethene Trichloroethene Vinyl Chloride cis, 1,2 DCE Dehalococcoides (DHC) tcaA Bav1
Legend
Vertical Migration AssessmentVertical Migration Assessment
•• Chlorinated SolventsChlorinated Solvents
•• Deep water column with multiple fracturesDeep water column with multiple fractures
•• Non cooperative geoNon cooperative geo--chemical conditionschemical conditions
MultiplMultiple e FracturFractureses
ImplementationImplementation
TreatmentTreatment
Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends
3/18/2015 6/22/2015 9/17/2015
PCE 28.7 18.6 1.7
TCE 1230 676 7.5
cDCE 133 23.2 61.5cDCE 133 23.2 61.5
VC 0 0 1.9
Ethane 0 0 0
iron 316 103 8260826082608260
SO4 51.4 46.2 19.6
Methane 0 0.34 2040204020402040
Microbial Ground Water ResultsMicrobial Ground Water Results
1.00E+03
1.00E+04
Microbial Populatons in GW
1.00E+00
1.00E+01
1.00E+02
3/1/2015 4/1/2015 5/1/2015 6/1/2015 7/1/2015 8/1/2015 9/1/2015
DHC BAV1 DHBt
DHC vs DepthDHC vs Depth
Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends
Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends
ImplementationImplementation
TreatmentTreatment
Donor WarsDonor Wars
Questions?Questions?