assay development and drug repurposing core
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Assay Development and Drug Repurposing Core
Drug Discovery Forum
Tom Dexheimer, Ph.D.Manager, MSU Assay Development and Drug Repurposing Core
May 14, 2015
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MSU Assay Development and Drug Repurposing Core
• To provide equipment and expertise for high-throughput screening techniques to MSU researchers
• To provide MSU researchers with access to cost-effective chemical library screening for identification of molecules to further interrogate particular biological processes or potential leads for drug development (or drug repurposing)
• To promote a training environment that will expose students to the techniques of high-throughput assay design, development, and HTS screen execution
• To provide assistance and/or the generation of preliminary data for grant/funding opportunities
Goals of the ADDRC
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Equipment – Liquid handling
Multidrop 384 Reagent Dispenser
• Peristaltic pump based cassette dispenser • Dispense 96- and 384-well plates• 5 to 395 mL volume range (5 mL
increments)
EL406 Microplate Washer Dispenser
• Peristaltic pump based cassette dispenser
• 1 mL and 5 mL cassettes• 96-well washer module• Syringe pumps dispensers• Variable dispense and
aspiration rates
Biomek FX Laboratory Automation Workstation• Automated/programmable robotic dual arm
system with 96-multichannel pipettor and Span-8 pipettor (8 individually controlled channels)
• Equipped with 384-pin tool• Small volume plate to plate transfers (i.e. compound plate to assay plate)• Volume range - 100 nL to 200 nL
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Cytation 3 Cell Imaging Reader
Equipment – Readers
• Automated digital microscopy• Objectives: 4x and 20x (currently available)• Brightfield, 365 nm, 465 nm, 590 nm• Temperature and CO2/O2 control
Synergy Neo Multi-mode Reader• Combines filter-based optical system and monochromator optics,
providing both excellent performance and assay versatility• Detection modes: Fluorescence, time-resolved fluorescence,
fluorescence polarization, Alpha, luminescence, UV-visible absorbance
• Multi-plate stacker (30 plates)
Functional Drug Screening System FDSS/µCELL• Kinetic plate reader with an
integrated dispensing head and imaging-based detector
• High-speed digital CCD camera• 0.5 s to 100 s/data point • 480 nm /540 nm• 96- or 384-well compatible• Ca2+, cAMP, membrane
potential, etc.
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Chemical Libraries and Informatics
GREENScreen• Integrated compound management and
high throughput screening data storage and analysis system
Chemical Libraries• 6571 compounds
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MSU Drug Discovery
Drug Discovery listserv ([email protected])• Bring together researchers from all disciplines at MSU who are interested
or involved in drug discovery and development
Drug Discovery Seminar Series• Fridays from 9:00 to 10:00 AM in B448 Life Science Building
• http://phmtox.msu.edu/core-facilities/Assay/DDIG.htm
Michigan Drug Discovery Symposium
• SAVE THE DATE September 9th-10th
Contact InformationTom Dexheimer, Ph.D. B411 Life Science BuildingEmail: [email protected]: 517-884-4998
*The ADDRC facility was established through the generous support of the Vice President for Research and Graduate Studies, the Pharmacology and Toxicology Department, CNS, COM, CHM, CVM, and AgBioResearch.