Benzofuran pyran compound rescues rat and human osteoblast from lipotoxic effect of

palmitate by inhibiting lipid biosynthesis and promoting stabilization of RUNX2

Authors: Ashish Kumar Tripathi1, Divya Rai1, Priyanka Kothari1 Pragati Kushwaha2, Shradha

Sinha1, Anirban Sardar1 Koneni V. Sashidhara2, Ritu Trivedi1*

*Corresponding Author: Ritu Trivedi

Endocrinology Division

CSIR-Central Drug Research Institute

B.S. 10/1, Sector 10, Jankipuram Extension

Sitapur Road, Lucknow, Uttar Pradesh 226031, INDIA

Phone: 91-522-2771940-42 (Ext. 4397)

Fax: 91-522-2771941

Email- ritu_trivedi@cdri.res.in

ritu_pgi@yahoo.com

S 1 Result

Screening of Benzofuran Pyran Compounds on palmitate induced lipotoxic in vitro

model

Compound 4j, neither increases the cell viability nor osteoblast differentiation (Fig.S1A and

B). Compound 4k enhanced cell viability at 500pm and 1.0µM (Fig.S1C) but failed to

enhance osteoblast differentiation in presence of palmitate (Fig.S1D).

(B)

1

ALP activity (OD 405)

Palmitate 250μM

##

(A)

(D)

Figure legend

Fig S1 Compound 4j did not rescue osteoblasts from lipotoxic of palmitate (A) and nor

increase ALP activity in the presence of palmitate (B). Compound 4k significantly increased

cell viability at 500pm and 1µM concentration (C) but failed in increasing ALP activity (D).

Data were presented as the mean ± SE, # p˂0.05, ## p˂ 0.005, ### p˂0.0005 versus control

group and *p˂0.05, **p˂0.005, ***p˂0.0005 versus palmitate group.

2

#

ALP activity (OD 405)

Palmitate 250μM

4k(C)