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Page 1: Appendix - shodhganga.inflibnet.ac.inshodhganga.inflibnet.ac.in/bitstream/10603/3370/17/17_appendix.pdf · Appendix Appendix Rotary Evaporator and GC-MS The JEOL GCMATE II GC-MS with

Appendix

Page 2: Appendix - shodhganga.inflibnet.ac.inshodhganga.inflibnet.ac.in/bitstream/10603/3370/17/17_appendix.pdf · Appendix Appendix Rotary Evaporator and GC-MS The JEOL GCMATE II GC-MS with

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Appendix

Rotary Evaporator and GC-MS

The JEOL GCMATE II GC-MS with Data system is a high resolution, double

focusing instrument. Maximum resolution: 6000 Maximum calibrated mass: 1500 Daltons.

Source options: Electron impact (EI); Chemical ionization (CI)

Plant Collected Area and Taxonomically Identified and Authenticated

by Rev Dr S John Britto SJ, Director, The Rapinat Herbarium, St

Joseph’s College (Autonomous), Tiruchirappalli, Tamil Nadu, India.

Page 3: Appendix - shodhganga.inflibnet.ac.inshodhganga.inflibnet.ac.in/bitstream/10603/3370/17/17_appendix.pdf · Appendix Appendix Rotary Evaporator and GC-MS The JEOL GCMATE II GC-MS with

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Experimental Flow Chat

Animal House Certification by IAEC

Page 4: Appendix - shodhganga.inflibnet.ac.inshodhganga.inflibnet.ac.in/bitstream/10603/3370/17/17_appendix.pdf · Appendix Appendix Rotary Evaporator and GC-MS The JEOL GCMATE II GC-MS with

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Animal House:

(IAEC No.: 743/03/abc/CPCSEA dt 3.3.03 - approval no.:

PhD2/2009-2010)

Group I Group II

Group III Group IV

2D Electrophoresis (Biorad) and MALDI-TOF-MS (Bruker-Daltonics, Germany)

2-DE

MALDI-TOF-MS

Page 5: Appendix - shodhganga.inflibnet.ac.inshodhganga.inflibnet.ac.in/bitstream/10603/3370/17/17_appendix.pdf · Appendix Appendix Rotary Evaporator and GC-MS The JEOL GCMATE II GC-MS with

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2D Software Melanie Viewer 7.0 and MASCOT Analysis (PMF)

MASCOT Analysis (PMF)

Page 6: Appendix - shodhganga.inflibnet.ac.inshodhganga.inflibnet.ac.in/bitstream/10603/3370/17/17_appendix.pdf · Appendix Appendix Rotary Evaporator and GC-MS The JEOL GCMATE II GC-MS with

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Gelscape-A Web Based Gel Viewing and Annotation System (http://www.gelscape.ualberta.ca:8080/htm/index.html)

FLICKER - Comparing Images (http://www-lmmb.ncifcrf.gov/flicker/)