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Appendix
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Appendix
Rotary Evaporator and GC-MS
The JEOL GCMATE II GC-MS with Data system is a high resolution, double
focusing instrument. Maximum resolution: 6000 Maximum calibrated mass: 1500 Daltons.
Source options: Electron impact (EI); Chemical ionization (CI)
Plant Collected Area and Taxonomically Identified and Authenticated
by Rev Dr S John Britto SJ, Director, The Rapinat Herbarium, St
Joseph’s College (Autonomous), Tiruchirappalli, Tamil Nadu, India.
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Experimental Flow Chat
Animal House Certification by IAEC
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Animal House:
(IAEC No.: 743/03/abc/CPCSEA dt 3.3.03 - approval no.:
PhD2/2009-2010)
Group I Group II
Group III Group IV
2D Electrophoresis (Biorad) and MALDI-TOF-MS (Bruker-Daltonics, Germany)
2-DE
MALDI-TOF-MS
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2D Software Melanie Viewer 7.0 and MASCOT Analysis (PMF)
MASCOT Analysis (PMF)
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Gelscape-A Web Based Gel Viewing and Annotation System (http://www.gelscape.ualberta.ca:8080/htm/index.html)
FLICKER - Comparing Images (http://www-lmmb.ncifcrf.gov/flicker/)