apoptosis. necrosis and apoptosis apoptosis morphological
TRANSCRIPT
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Apoptosis
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Necrosis and Apoptosis
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Apoptosis Morphological
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Morphological
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Necrosis Biochemical
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Apoptosis Biochemical
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Agarose gel electrophoresis of DNA isolated from promyelocytic leukemia cells (HL-60) were either untreated (Lane 1) or treated with 0.5 mg/ml actinomycin D (Lane 2). DNA Markers (M) were electrophoresed as a base pair reference.
DNA ladder
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nucleosome
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FACS DNA
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Anexin V
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Anexin V
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Necrosis physical
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Apoptosis physical
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Physical significance
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3. Detection of Apoptosis
• Morphological
• DNA electrophoresis
• DNA 断裂的原位末端标记 TUNEL 测定法, Terminal deoxynucleotid
yl transferase(TdT)-mediated dUTP end labeling ,末端脱氧核苷酸移换酶介导的 dUTP缺口末端标记测定法
• Flowmetery
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3.Regulation of Apoptosis
⑴ Genes for proliferation
c-myc
ras
⑵ For survival
bcl-2
c-kit
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⑶ proliferation inhibitors
p53
Rb
⑷ death promotors
Bax
ICE
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4.p53 and apoptosis
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2002 Press Release
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Laureates
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Ap-overview
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