Copyright © 2012 Abcam, All Rights Reserved.

Apoptosis and Cancersignaling pathways

Discover more at abcam.com/biochemicals

Mitochondria

Bcl-2

Cyt-c

Apaf-1

Bcl-2 inhibitors

Caspase 9

Caspase 3

Cellular stress,Death stimulus

Apoptosis

Apoptosis

Gene regulation

Bcl-2

Cyt-c

Bax

Apaf-1/Caspase 9

DNA damage Cellular stress

p53

Mitochondria

sisAp osop ptotopApppopp sisApAp osppoptosostoptppop

GGene r egegulationn

Cell cycle arrest, cellgrowth inhibition,metabolic stress

inhibition

Apoptosis Transcription

NF-κB

BID IKK

BAX

Cyt-c

Caspase 3

TRADD

TRAF-2Pro-caspase 8

FADD

Death domain

TNFR1 receptorsTNFR

TNFα

Degradation

TRAIL

NF-κB PathwayApoptosis Pathway

ApoptosisPARP cleavage

Smac-mimetic(SH122)

NF-κB

Bid IκBα

Degr dad tation

IκBα

I

ReIA

Smac

Caspase 8

Caspase 9

Caspase 3

Cyt-cXIAP

cIAP-1

DR

4/5

Mitochondria

Bcl-2 signaling in cellular apoptosis p53 signaling in apoptosis and gene transcription

Death receptor signaling in apoptosis and gene transcription Inhibitors of apoptosis signaling in cellular apoptosis

The bcl-2 family consist of pro- and

anti-apoptotic proteins involved in

intrinsic mitochondrial cell death. They

regulate the release of apoptotic

factors into the cytoplasm including

cytochrome C and Apaf-1, by mediating

outer membrane permeabilisation. This

leads to activation of caspase-9 and

the resulting caspase cascade.

Death receptors (DRs) belong to

the tumour necrosis factor (TNF)

family of cytokines which induce

apoptosis. These receptors can

activate caspases within seconds

causing apoptosis.

p53 is a critical tumour suppressive

transcription factor which responds to

cellular stress by triggering cell cycle

arrest and apoptosis through

transcriptional regulation.

Inhibitors of apoptosis (IAPs) are a

conserved group of proteins containing

Baculovirus-IAP repeats involved in the

regulation of apoptosis as well as a

number of other cellular processes.

300_12_ED A&C Pathways Card:A4 18/05/2012 11:46 Page 1

300_

12_E

D

Apoptosis and Cancer related products in the Abcam Biochemicals range:

Discover more at abcam.com/biochemicals

*Sold under exclusive licence from Children’s Medical Research Institute and Newcastle Innovation Ltd. Dynole-34-2™, Dynole-31-2™, MiTMAB™, OcTMAB™, Iminodyn-22™ and Iminodyn-17™ are trademarks of Children’s Medical Research Institute and Newcastle Innovation Ltd.”

Product Biological description Purity Product code

MAP kinases

Anisomycin Protein synthesis inhibitor >98% ab120495

2-Methoxyestradiol Potent anti-tumor and anti-angiogenic agent >98% ab120653

PD 98059 MEK1 inhibitor >99% ab120234

SB 202190 Highly selective, potent, cell permeable p38 MAP kinase inhibitor >99% ab120638

SB 203580 p38 MAP kinase inhibitor >99% ab120162

SB 203580 hydrochloride p38 MAP kinase inhibitor; water soluble >99% ab120235

SL 327 MEK inhibitor >99% ab120082

SP600125 JNK inhibitor >98% ab120065

PD 0325901 Potent MEK 1 and 2 inhibitor >99% ab120639

PD 184352 Selective MEK inhibitor >99% ab120641

U0126 Selective MKK inhibitor >98% ab120241

CGP 57380 MNK inhibitor >98% ab120365

ZM 336372 Potent, selective c-Raf inhibitor >98% ab120428

Akt/PI3K

SC-66 Allosteric Akt inhibitor >99% ab120802

Luteolin Antioxidant, anti-inflammatory, anti-cancer flavonoid >99% ab120662

LY 294002 PI3 kinase inhibitor >99% ab120243

Wortmannin PI3 kinase inhibitor >98% ab120148

Phorbol 12-myristate 13-acetate PKC activator >99% ab120297

Ro 31-8220 mesylate Protein kinase inhibitor >98% ab120374

Rottlerin Kinase inhibitor >95% ab120377

Okadaic acid PP1 and PP2A inhibitor >98% ab120375

p53

Pifithrin-α-HBr p53 inhibitor >98% ab120478

Caspases

Z-D(OMe)E(OMe)VD(OMe)-FMK Cell permeable caspase-3 inhibitor >90% ab120488

Z-LE(OMe)VD(OMe)-FMK Cell permeable derivative of caspase-4 inhibitor Z-LEVD-FMK >90% ab120489

Z-VAD-FMK Irreversible general caspase inhibitor >98% ab120382

Nitrogen oxide

L-Arginine Precursor to nitric oxide synthesis >99% ab120750

(S)-Nitrosoglutathione NO donor ab120285

SNAP NO donor ab120014

Spermidine hydrochloride Endogenous polyamine. nNOS inhibitor. >99% ab120057

1400W Selective iNOS inhibitor >99% ab120165

Calcium signaling

K252a Highly potent inhibitor of protein and CAM kinases >99% ab120419

KN-62 CaM kinase II inhibitor. P2X7 antagonist. >98% ab120421

Cyclin-dependent kinase

Purvalanol A Potent CDK inhibitor >98% ab120304

Purvalanol B CDK2 inhibitor >98% ab120305

GSK

Phospho-Glycogen Synthase Peptide-2 GSK substrate >99% ab120079

SB 216763 Potent selective GSK-3 inhibitor >98% ab120202

GTPase

GTP GDP

Stalk AD PRDPH

Structural domains of dynamin, their function and the target sites of action of the MiTMAB™, Dynole™ and Iminodyn™ chemical series collections

GTPBinding

LipidBinding

Domain

InhibitorInteraction

• MiTMAB™• OcTMAB™• RTIL-13™

• Dynole-34-2™• Iminodyn-22™

RingAssembly

Phos/DephosProtein-Protein

World Exclusive!

Pitstop 2™ Clathrin inhibitor

Novel, selective, cell membrane permeable clathrin inhibitor.

Competitively inhibits clathrin terminal domain to selectively inhibit

clathrin mediated endocytosis (CME) (IC50 = 12 µM for inhibition

of amphiphysin association of clathrin TD). Interferes with

receptor mediated endocytosis (RME), entry of HIV and synaptic

vesicle recycling. (ab120687)

World Exclusive!

Dynamin inhibitors toolbox

The Dynamin Inhibitors Toolbox (ab120468) is a unique collection

of small molecule, potent and cell permeable dynamin inhibitors,

enabling characterization of dynamin and endocytosis. The

inhibitors have different mechanisms of action, target different

domains of dynamin, and are based on differing chemical scaffolds.

Negative controls for each chemical series are included.

Featured products

Product Biological description Purity Product code

300_12_ED A&C Pathways Card:A4 18/05/2012 11:46 Page 2