TNF

TNFR1

IL-1

IL-1RLPS

TRADD FADD TRAF2 TRAF3

NEMO

IKKα

IKBαp65

IKKβ

NEMO

IKKα IKKβ

myD88IRAK1/4 Tyr Kinase

Calpain

IRAK CK2

NIKTAB TAK

TRAF6

TRAF6

S32 S36

p50

IKBαp65

IKBαdegradation

Y42

S866

S870

p50

IKBαp65p50

Canonical PathwayIKK Independant

Pathway

HypoxiaH2O2 UV, Her2

CD40L

CD40

Non-canonicalPathway

IKBαdegradationub

p65p50

RELBp100

RELBp52

RELBp52

Proteosomaldegradation

Coactivator

p65p50 AC

TranscriptionalActivation

p65p50 TF

Promoter-targetingRegulation

Distinct Binding Sites

Corepressor

p65p50

TranscriptionalRepression

ub

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◣ Cytokines / Chemokines◣ Immunoreceptor◣ Cell adhesion molecules◣ Acute phase proteins◣ Stress response

◣ Cell surface receptors◣ Regulators of apoptosis◣ Growth factors and ligands◣ Early response genes◣ Transcription factors & regulators

◣ Lymphoid organogenesis◣ B cell maturation◣ Humoral immunity

Nuclear factor kappa B (NFkB) is a protein complex that controls large number of genes responsible for the regulation of apoptosis, inflammation, tumorigenesis and a variety of immune responses. NF-kB is sensitive and responsive to stimuli such as cytokines, stress, UV, low oxygen, bacterial or viral infections.

Antibody˙Duo˙ELISA Kit

NFkB Pathway

ub

c-IAP1/2

www.arigobio.com

NFkB Signaling Pathway Related ProductsProduct NameCatalog No. Clonality Applications Reactivity

ARG51780IKK beta pY199

Rel or NF-kappaB (NF-kB) proteins comprise a family of structurally-related eukaryotic transcription factors that are involved in the control of a large number of normal cellular and organismal processes, such as immune and inflammatory responses, developmental processes, cellular growth, and apoptosis.Up-regulation of phospho-IKK, phospho-p38, phospho-JNK and down-regulation of IKB alpha is often associated with the activation of NF-kB downstream signaling pathway.

Product Description:

E

E

E

E

E

E

E

E

ARG51780

ARG54059

ARG51850

ARG51807

ARG51651

IKK beta pY199

IkB alpha antibody (N-term)

p38 MAPK pT180/Y182

JNK1/2/3 pT183/Y185

IKB alpha pS32/36

Rabbit pAB

Mouse mAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

ICC/IF

WB

WB

WB, ICC/IF

WB, IHC, ICC/IF

H,M,R

H,M

H,M,R

H,M,R

H,M,R

50 µl

50 µl

50 µl

50 µl

50 µl

Component Name

Component cat no. Clonality Applications Reactivity Package

ARG51160IKB alpha antibody

ARG51651IKB alpha pS32 / 36 antibody

ARG51651

ARG54059

IKB alpha pS32/36

IkB alpha antibody (N-term)

Rabbit pAB

Mouse mAB

WB, IHC, ICC/IF

WB

H,M,R

H,M

50 µl

50 µl

ARG54369

ARG20070

ARG80154

ARG20066

ARG52619

ARG52770

ARG80130

ARG54127

ARG51660

ARG54686

ARG20319

ARG80237

ARG80204

ARG80504

ARG80712

ARG80196

ARG80503

ARG54631

ARG54348

ARG51015

ARG51519

ARG51520

ARG51017

ARG51276

ARG65677

ARG51514

ARG51516

ARG51664

BAFF antibody

BAFF antibody

BAFF ELISA Kit

Calpain1 antibody

Calpain1 antibody

CD40 antibody

CD40L ELISA Kit

FADD antibody

IKappaB alpha pY42 antibody

IKK beta antibody

IL-1a antibody

IL-1a ELISA Kit

IL-1a ELISA Kit

IL-1a ELISA Kit

IL-1b ELISA Kit

IL-1b ELISA Kit

IL-1b ELISA Kit

IRAK4 antibody

MYD88 antibody

NFkB p100/p52 antibody

NFkB p100/p52 pS866 antibody

NFkB p100/p52 pS870 antibody

NFkB p105 antibody

NFkB p105 antibody

NFkB p65 antibody

NFkB p65 pT254 antibody

NFkB p65 pT435 antibody

NFkB p65 pT505 antibody

WB, ICC/IF

WB

ELISA

WB

WB, IHC

IHC

ELISA

WB

WB, IHC

ELISA, ICC/IF, WB

WB, Neut

ELISA

ELISA

ELISA

ELISA

ELISA

ELISA

WB, ICC/IF

WB

WB, IHC

WB, IHC, ICC/IF

WB, IHC, ICC/IF

WB, IHC

WB, ICC/IF

IHC, WB

WB, IHC, ICC/IF

WB, IHC, ICC/IF

WB, IHC

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit mAB

Rabbit pAB

Mouse mAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Mouse mAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

H

H

H

H, M, R, Bo, P, Rb

H

H

H

H

H, M, R

H

H

R

M

H

R

M

H

H

H, M, R

H, M, R

H, M, R

H, M, R

H

H, M, R

H, M

H, M, R

H, M, R

H, M, R

ARG51807JNK 1 / 2 / 3

(pT183 / Y185)

ARG51850P38 MAPK

(pT180 / Y182)

Component Name

Component cat no. Clonality Applications Reactivity Package

Hayden and Ghosh. (2012) Genes and Dev 26: 203-234Baeuerle and Henkel. (1994) Ann Rev Immunol 12: 141-179

HeLa cells

Human breastcarcinoma tissue

NF-kB Activation Antibody Panel (ARG30205)

Phospho-IKB alpha Antibody Duo (Total, pS32/36) (ARG30037)

ARG65351Goat anti-Rabbit IgG Antibody (HRP)

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NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with RELA (p65). Four transcript variants encoding different isoforms have been found for RELA. (provided by RefSeq, Sep 2011)NFkB (p65) has multiple phosphorylation site with different regulations and functions. Phosphorylation at Serine 536 is mediated by IKK beta and/or IKK alpha in the cytoplasm and it is required for p65 activation. Phosphorylation on Ser276 of p65 in the nucleus enhances its ability to recruit histone acetyltransferases such as cAMP response element-binding (CREB)-binding protein (CBP) and p300 and to displace p50–histone deacetylase (HDAC)-1 complexes from DNA. In addition, IL1 beta and TNF alpha stimulate p65 phosphorylated at Ser529 through CK2 protein.ARG30029, ARG30030 and ARG30031 NFkB phosphor Duos include antibodies react total p65 protein and p65 phosphorylated at Ser276 or Ser529 or Ser536. It is useful for the user in p65 related functional studies.

NFkB Target GenesProduct NameCatalog No. Clonality Applications Reactivity

Product NameCatalog No. Clonality Applications Reactivity

NIK antibody

RelB pS573 antibody

TAB1 antibody

TAK1 antibody

TNF-alpha antibody

TNF-alpha ELISA Kit

TNF-alpha ELISA Kit

TNF-alpha ELISA Kit

TNFR1 antibody

TRADD antibody

TRAF2 antibody

TRAF3 antibody

Rabbit pAB

Rabbit pAB

Mouse mAB

Goat pAB

Mouse mAB

Mouse mAB

Rabbit pAB

Goat pAB

Rabbit pAB

WB, ELISA

WB, IHC

WB

WB

FACS, IHC, ICC/IF, FuncSt

ELISA

ELISA

ELISA

FACS, IP, IHC, FuncSt

IHC

WB, IHC

WB

H

H, M

H

H

H, Pri, P

R

M

H

H

H

H

H, M, R

E

E

E

E

ARG54322

ARG51741

ARG54074

ARG63986

ARG65482

ARG80241

ARG80206

ARG80120

ARG65452

ARG53680

ARG64172

ARG20163

Bax antibody

Bcl-2 antibody

Bcl-xL antibody

BDNF antibody

Bim antibody

Bmi1 antibody

BMP-2 ELISA Kit

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

Mouse mAB

ELISA, IHC-P, WB

WB, IHC, IP

WB

WB

WB, ICC/IF

WB

ELISA

H, M, R

H, M, R

H, M, R

H

H

H

H

ARG65612

ARG20424

ARG51057

ARG20307

ARG54406

ARG53981

ARG80164

D

D

D

ARG51518

ARG51708

Rabbit pAb

Rabbit pAb

WB, IHC-P, ICC/IF

WB, IHC-P, ICC/IF

H,M,R

H,M,R

50 µl

50 µl

Component NameComponent

cat no. Clonality Applications Reactivity Package

ARG30029Phospho-NFkB Antibody Duo (pS529, pS536)

NFkB-p65 pS536 antibody

NFkB-p65 pS529 antibody

ARG51013

ARG51518

Rabbit pAb

Rabbit pAb

WB, IHC-P

ICC/IF, IHC-P, WB

H,M,R

H,M,R

50 µl

50 µl

Component NameComponent

cat no. Clonality Applications Reactivity Package

ARG30248Phospho-NFkB Antibody Duo (Total, pS536)

NFkB p65 antibody

NFkB p65 pSer536 antibody

ARG51708NFkB-p65 p-Ser529 antibody

Human breastcarcinoma tissue

ARG51518NFkB-p65 p-Ser536 antibody

HeLa cells

ARG51013NFkB p65 antibody

Human breastcarcinoma tissue

Phospho-NFkB p65 Antibody Duo

ARG65048PRDM-1 antibody

Human tonsil Sw480 cells HeLa cellsHuman colon

adenocarcinoma

ARG53981Bmi1 antibody

ARG54038ICAM1 antibody

ARG52901COX-2 antibody

ARG20345MIP-1a antibody

ARG51081p53 antibody

NFkB Target GenesProduct NameCatalog No. Clonality Applications Reactivity

E

E

E

E

E

E

E

E

E

E

ARG55523

ARG51034

ARG52902

ARG80195

ARG80825

ARG53974

ARG63244

ARG80143

ARG80229

ARG80185

ARG54038

ARG80116

ARG80493

ARG53370

ARG53340

ARG80908

ARG20345

ARG80174

ARG52352

ARG62568

ARG62512

ARG10519

ARG65048

ARG20190

ARG65593

ARG20161

ARG80120

ARG80134

BMP-4 antibody

c-MYC antibody

COX2 antibody

CRP ELISA Kit

EPO ELISA Kit

Fibronectin antibody

GATA3 antibody

G-CSF ELISA Kit

GM-CSF ELISA Kit

GRO beta / CXCL2 ELISA Kit

ICAM-1 antibody

IFN-gamma (HS) ELISA Kit

IGFBP-2 ELISA Kit

iNOS antibody

IRF4 antibody

MIP-1 alpha ELISA kit

MIP-1a antibody

MIP2 ELISA kit

NDMAR1 antibody

NGF antibody

NRG1 antibody

p53 antibody [Pab1801]

PRDM1/BLIMP1 antibody

P-selectin antibody

STAT5A antibody

TLR9 antibody

TNF-alpha ELISA kit

VCAM1 ELISA kit

WB, IHC-P

WB, IHC

IHC

ELISA

ELISA

IHC

WB, IHC

ELISA

ELISA

ELISA

WB

ELISA

ELISA

IHC

WB, IHC

ELISA

WB, IP

ELISA

WB, IP

ICC/IF, FACS, IHC

WB, IP, IHC

ChIP, ELISA, FACS, ICC/IF, IHC, IP, RIA, WB

WB, IHC

WB, IHC

WB, IHC

WB

ELISA

ELISA

Rabbit pAB

Rabbit pAB

Rabbit mAB

Rabbit pAB

Goat pAB

Mouse mAB

Rabbit pAB

Rabbit mAB

Rabbit pAB

Mouse mAB

Mouse mAB

Mouse mAB

Mouse mAB

Goat pAB

Rabbit pAB

Rabbit pAB

Rabbit pAB

H, M

H, M, R

H, M, R

H

H

H

H

H

M

H

H

H

H

H, M, R

H

R

M, R

H

M, R

H, R, Rb, Cat, Fer, Pri

H, M, R

H, M, R

H

H, M, R

H, M, R

H, M, R, P

H

H

ELISA Kits Duo/Panel also available Species Reactivity: H- Human; M- Mouse; R- Rat; Rb- Rabbit; Pri- Primate; P- Pig; Mk- Monkey; Bo- BovineE D

No.22, Ln. 227, Gongyuan Road., Hsinchu City 300, TaiwanE : info@arigobio.com / T : +886 (3) 5621738 / F : +886 (3) 561 3008

www.arigobio.com

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