antibodies for neuroscience research - vinci …...10224 gpr55 polyclonal antibody fc, icc, wb bo,...

Antibodies forNEUROSCIENCE RESEARCH

Table of Contents

2 Cyclic Nucleotide Gated/Ion Channels

3 Neurotransmitter Receptors

4 Neurotransmitter Synthesis, Signaling & Degradation

5 Synapse Mechanics

5 Endocrinology

5 pan-Cytokeratins

6 Prion Proteins

6 Optineurin

Antibodies forNEUROSCIENCE RESEARCHCayman has antibodies for diverse neuroscience research applications, including factors related to synapse structure, excitatory and

inhibitory neurotransmission, gated ion channels and transporters, and neurodegenerative diseases. We also offer a select group of tools

inhibitors and substrates, receptor agonists and antagonists, sensitive immunoassays and inhibitor screening assays, to study topics such as

cell death and proliferation, stem cell differentiation, inflammation and toxicity, endocrinology, and pain management. A select group of

psychoactive drugs including cannabinoids, cathinones, phenethylamines, amphetamines, indanes, and tryptamines are also available to

authorized researchers and forensic scientists.

More information for these products can be found at www.caymanchem.com/Neuro

Cayman Chemical • Ann Arbor, MI 48108 • 800.364.9897 • www.caymanchem.com

Ordering Information

Orders are accepted by telephone, fax, mail, e-mail, or via the Cayman Chemical website. We will accept telephone orders Monday through Friday from 8 AM to 6 PM EST. All orders received by 1 PM EST will be shipped the same day if stock is available (Monday through Thursday only). Confirming purchase orders must be clearly marked as such to avoid possibility of duplication.

Domestic ShipmentsIn most instances we ship FedEx Standard Overnight Delivery (not available to all locations), with delivery by 3:30 PM of the next business day. Product availability may vary. Local delivery is available for the Ann Arbor area only. Other shipping options will be considered upon request, but can be granted only under conditions that will ensure the quality of the product. Freight is prepaid and added to the invoice. Please inquire at the time of order for an estimate of the freight charges. If you wish us to ship collect, please supply a valid account number when ordering. Please address all orders to:

Cayman Chemical Company Toll-free Phone: (800) 364-98971180 E. Ellsworth Road Fax: (734) 971-3640Ann Arbor, MI 48108 USA E-mail: [email protected]: (734) 971-3335 www.caymanchem.com

Include the following information with your order:1. Catalog number, description, size, and quantity desired.2. Complete shipping address. (Delivery is not available to post office box numbers.)3. A complete billing address.4. A purchase order number or major credit card (Visa, MasterCard, or American Express), account number, and expiration date.5. Name of the end user.

Terms1. U.S. funds only, drawn on a U.S. bank.2. Net 30 days.3. F.O.B. Ann Arbor, Michigan, U.S.A.4. Bank fees and wire transfer fees are not to be deducted from the invoice amount.

ReturnsProducts cannot be returned without prior authorization from Cayman Chemical Company. Please contact our Customer Service Department for return shipping instructions. Custom orders and radioactive material cannot be accepted for return credit if due to a customer’s error.

Technical AssistanceTechnical assistance is available from 8 AM to 5:30 PM EST. If inquiring about a purchased product, please provide the catalog number, lot number, and date of purchase to our technical staff so they may answer your questions quickly. Technical assistance may be reached toll free at 888-526-5351, via e-mail at [email protected], or on the web at www.caymanchem.com/techserv.

Use of Research ProductsThe products in this catalog are not for human or veterinary disease diagnosis or therapeutic drug use. They should be used only by technically qualified individuals or those under their direct supervision. Any individual working directly with these products should have free access to the applicable Material Safety Data Sheet (MSDS) and should read and understand it completely prior to use. Please contact our Customer Service Department or visit the specific product page on our website if you require additional copies of any MSDS.

Patent DisclaimerThe end-user assumes full responsibility for appropriate licensing and/or non-infringement for any proprietary claim or patent.

[NEUROSCIENCE RESEARCH]

ELISA enzyme-linked immunosorbent assay |FC flow cytometry | ICC immunocytochemistry | IF immunofluorescence |IHC immunohistochemistry |IP immunoprecipitation | WB western blot4

Cyclic Nucleotide Gated/Ion ChannelsItem No. Item Name Applications Species Reactivity/Specificity

13702 Cav1.2 Calcium Channel Monoclonal Antibody (Clone S57-46) ICC, IF, IHC, WB H, Mo, R

13703 Cav1.3 Calcium Channel Monoclonal Antibody (Clone S38-8) ICC, IF, IHC, WB H, Mo, R

13706 Cav1.3 Calcium Channel Monoclonal Antibody (Clone S48A-9) ICC, IHC, WB H, Mo, R

13704 Cav3.2 Calcium Channel Monoclonal Antibody (Clone S55-10) ICC, IHC, WB H, Mo, R

13700 Cavβ1 Calcium Channel Monoclonal Antibody (Clone S7-18) ICC, IHC, WB H, Mo, R

13701 Cavβ4 Calcium Channel Monoclonal Antibody (Clone S10-7) ICC, IF, IHC, WB H, Mo, R

13705 HCN1 Cyclic Nucleotide-gated Channel Monoclonal Antibody (Clone S70-28) ICC, IHC, IP, WB H, Mo, R

13707 HCN2 Cyclic Nucleotide-gated Channel Monoclonal Antibody (Clone S71-37) ICC, IHC, IP, WB H, R

13708 HCN3 Cyclic Neuclotide-gated Channel Monoclonal Antibody (Clone S141-28) ICC, IHC, WB Mo, R

13709 HCN4 Cyclic Nucleotide-gated Channel Monoclonal Antibody (Clone S114-10) ICC, IHC, WB Mo, R, H

13711 KCNQ1 Potassium Channel Monoclonal Antibody (Clone S37A-10) ICC, IHC, IP, WB H, Mo, R

13712 KCNQ2 Potassium Channel Monoclonal Antibody (Clone S26A-23) ICC, IHC, IP, WB H, Mo, R

13713 KCNQ4 Potassium Channel Monoclonal Antibody (Clone S43-6) ICC, IP, WB H, Mo, R

13714 Kir2.1 Potassium Channel Monoclonal Antibody (Clone S21-32) IHC, WB H, Mo, R



13717 Kv3.1b Potassium Channel Monoclonal Antibody (Clone S16B-8) IHC, WB H, Mo, R

13718 Nav1.7 Sodium Channel Monoclonal Antibody (Clone S68-6) ICC, IP, WB H, Mo, R

13719 TRPC4 Calcium Channel Monoclonal Antibody (Clone S77-15) ICC, IP, WB H, Mo, R

13720 TRPM7 Ion Channel Ser/Thr Kinase Monoclonal Antibody (Clone S74-25) ICC, IP, WB H, Mo, R

13721 TRPV3 Channel Monoclonal Antibody (Clone S15-4) ICC, IP, WB H, Mo, R


5Bo bovine | C canine | CE C. Elegans | Ch chicken | Gt goat| H human | Ha hamster|Mo mouse | NHP non-human primate| P pig | R rat | Rb rabbit | Sh sheep | X xenopus | Ze zebrafish

Neurotransmitter ReceptorsItem No. Item Name Applications Species Reactivity/Specificity

10011454 Adenosine Receptor A2A Monoclonal Antibody (Clone 7FG-G5-A2) WB H

10006590 CB1 Receptor (C-Term) Polyclonal Antibody IHC, WB H, Mo, R

101500 CB1 Receptor Polyclonal Antibody WB H, Mo, R

101550 CB2 Receptor Polyclonal Antibody IHC, WB H, Mo

10010712 CB2 Receptor Polyclonal FITC Antibody FC, IF, WB H, Mo

10007660 GPR35 Polyclonal Antibody IHC, WB H, P, Mo

10224 GPR55 Polyclonal Antibody FC, ICC, WB Bo, H, Mo

10600 GABAA Receptor δ-subunit (N-Term) Polyclonal Antibody IHC, IP, WB Mo, R

10602 GluR1 (Phospho-Ser831) Polyclonal Antibody WB R

10601 GluR1 (Phospho-Ser845) Polyclonal Antibody IHC, WB R

10009399 Glycine Receptor Polyclonal Antibody IHC, WB H, Mo, R

10607 NMDA Receptor NR1 Subunit Monoclonal Antibody IP, WB Mo, R

10608 NMDA Receptor NR2A Subunit Polyclonal Antibody IHC, IP, WB H, R, Mo

10009759 NMDA Receptor NR2B Subunit (Phospho-Tyr1252) Polyclonal Antibody WB H, R; Bo, Ch, C, Mo, NHP, Ze(expected)



10609 NMDA Receptor NR2B Subunit Polyclonal Antibody IHC, IP, WB H, Mo, R

Retrograde Signaling through Cannabinoid ReceptorsThe bioactive agent from Cannabis, Δ9-tetrahydrocannabinol (THC),

activates two distinct cannabinoid (CB) receptors, CB1 and CB2, to elicit

a wide array of effects on mood, appetite, immunity, memory, and

pain perception. The receptors CB1 and CB2 are activated naturally by

endogenous CBs (endoCBs), including 2-arachidonoyl glycerol (2-AG).

While the CB2 receptor is abundant on leukocytes and has key roles in

immunoregulation and pain, the CB1 receptor predominates throughout

the central nervous system. Both are Gi-linked receptors, so that

activation blocks the action of adenylate cyclase, preventing signaling

through cyclic AMP. In the neuronal synapse, endoCBs like 2-AG act

in a retrograde fashion. That is, 2-AG is synthesized and secreted by

post-synaptic neurons and activate CB1 receptors at the presynaptic

axon terminal. Activation of CB1 then modulates signaling within the

presynapse, producing either a transient or prolonged reduction in the

release of neurotransmitters. For example, in the hippocampus, CB1

receptors are more abundant on inhibitory γ-aminobutyric acid (GABA)

terminals than on excitatory glutamate terminals. Activation of CB1 by

2-AG from post-synaptic terminals strongly decreases the release of the

inhibitory neurotransmitter GABA and less effectively reduces the release of the stimulatory glutamate. Prolonged inhibition of glutamate

signaling, as may occur with overproduction of endoCBs, may impair hippocampus-dependent learning and memory. Through its many

variable effects on several different types of neurotransmitters, CB1 signaling confers additional plasticity in neuronal signaling.

Axon Terminal

Dendrite

2-AGGlut

2-AG

γ PLC

β

DAG

IP3

DAG lipase

mGluR5NMDAR

ExcitatorySynapse

GlutGlut

GABA GABA

InhibitorySynapse

GABAA

Cl-

Ca2+

Ca2+ Cl-

CB1CB1

Gα

Synthesis and retrograde action of endocannabinoids



Neurotransmitter Synthesis, Signaling & DegradationItem No. Item Name Applications Species Reactivity/Specificity

10011438 CaMKII (Phospho-Thr286/Thr287) Monoclonal Antibody (Clone 22B1) ELISA, IF, IP, WB R

10011437 CaMKII Monoclonal Antibody (Clone 6G9) ELISA, IF, IHC, IP, WB Mo, R, Bo

10603 DARPP-32 (Phospho-Thr34) Polyclonal Antibody WB R

10009370 Dopamine β-hydroxylase (C-Term; human) Polyclonal Antibody WB H, Mo, NHP

10009371 Dopamine β-hydroxylase (N-Term; human) Polyclonal Antibody WB H, Mo, NHP

10009372 Dopamine Transporter (C-Term) Polyclonal Antibody IHC, WB H, Mo, Mq

10009373 Dopamine Transporter (Extracellular Loop 2) Polyclonal Antibody IHC, WB H, Mk

101600 Fatty Acid Amide Hydrolase Polyclonal Antibody IHC, WB H, R, Mo, Ferret

10009400 Gap-43 (Phospho-Ser41) Polyclonal Antibody WB R; Bo, C, Ch, Finch, H, Mo, NHPs, X, Ze(expected)

10212 Monoacylglycerol Lipase (FL) Polyclonal Antibody ICC, IHC, WB H, R, Mo, Ferret

100035 Monoacylglycerol Lipase Polyclonal Antibody IHC, WB H, Mo, Bo, R

160870 nNOS Polyclonal Antibody ICC, IHC, IP, WB H, R; (-) eNOS, iNOS

10009398 Tryptophan Hydroxylase (Phospho-Ser260) Polyclonal Antibody WB H, R; Bo, C, Ch, Mo, Ze(expected)

10009397 Tryptophan Hydroxylase (Phospho-Ser58) Polyclonal Antibody WB Rb; Bo, C, H, Mo, NHP, R, X, Ze(expected)

10009396 Tryptophan Hydroxylase Polyclonal Antibody IHC, WB H, R; Other mammals (expected); (-) Rb tryptophan

10009413 Tyrosine Hydroxylase (Phospho-Ser31) Polyclonal Antibody IF, IHC, WB Mo, R

10009414 Tyrosine Hydroxylase (Phospho-Ser40) Polyclonal Antibody IF, IHC, WB Mamalian, Non-mamalian

10604 Tyrosine Hydroxylase Polyclonal Antibody IF, IHC, WB Mammalian



Synapse Mechanics Item No. Item Name Applications Species Reactivity/Specificity

10009785 Dynamin (Phospho-Ser774) Polyclonal Antibody WB R

10009786 Dynamin (Phospho-Ser778) Polyclonal Antibody WB R

10011435 PSD95 Monoclonal Antibody (Clone 6G6) ICC, WB Mo, R, Bo

10011436 PSD95 Monoclonal Antibody (Clone 7E3) WB Mo, R, Bo

10009506 PSD95 Polyclonal Antibody WB R, Mo; Bo, H, NHPs, Ze(expected)

10606 Synapsin I Polyclonal Antibody IF, IHC, IP, WB H, Mo, R

10605 Synapsin I Polyclonal Antibody (neat serum) WB H, Mo, R

EndocrinologyItem No. Item Name Applications Species Reactivity/Specificity

13492 Androgen Receptor (Phospho-Ser210,213) Monoclonal Antibody (Clone 156C135.2) WB, IHC H, Mk

10009762 Progesterone Receptor (Phospho-Ser190) Monoclonal Antibody IHC, WB H

10009763 Progesterone Receptor (Phospho-Ser294) Monoclonal Antibody IHC, WB H; Mo, NHP, R (expected)

10011442 FKBP52 Monoclonal Antibody (Clone Hi52C) IHC, IP, WB C, Ha, H, Mo, R

10004549 11β-Hydroxysteroid Dehydrogenase (Type 2) Polyclonal Antibody ICC, WB H, Mo, R

10008856 GnRHa Polyclonal Antibody ELISA not available

pan-CytokeratinsItem No. Item Name Applications Species Reactivity/Specificity

10004600 Cytokeratin Monoclonal Antibody (Clone C-11) FC, ICC, IHC, WB H cytokeratin 4, 5, 6, 8, 10, 13, 18

10568 Cytokeratin Monoclonal APC Antibody (Clone C-11) FC H cytokeratin 4, 5, 6, 8, 10, 13, 19

10349 Cytokeratin Monoclonal FITC Antibody (Clone C-11) FC, IF H cytokeratin 4, 5, 6, 8, 10, 13, 20

10478 Cytokeratin Monoclonal PE Antibody (Clone C-11) FC, IF H cytokeratin 4, 5, 6, 8, 10, 13, 21



Prion ProteinsItem No. Item Name Applications Species Reactivity/Specificity

10005517 Doppel Polyclonal Antibody IHC, WB H, Mo, R

10009034 Prion Protein Monoclonal Antibody (BAR 221) ELISA, FC, IHC, WB Bo, H, Mo, Sh

10009030 Prion Protein Monoclonal Antibody (Clone 11C6) ELISA, FC, WB H, Mo

189710 Prion Protein Monoclonal Antibody (Clone 12F10) IHC, WB H, Bo, Sh; (-) Ha, Mo

10009025 Prion Protein Monoclonal Antibody (Clone 2G11) IHC Gt, Sh, Bo; (-) H, Ha

189760 Prion Protein Monoclonal Antibody (Clone 8G8) IHC H, Ha, Bo, Sh, Mo

10009035 Prion Protein Monoclonal Antibody (Clone BAR 224) ELISA, FC, IHC, WB Sh; (-) Bo, H, Mo

10009036 Prion Protein Monoclonal Antibody (Clone BAR 233) ELISA, FC, WB H, Mo, Sh

10009037 Prion Protein Monoclonal Antibody (Clone BAR 236) ELISA, FC, WB H, Mo, Sh

189750 Prion Protein Monoclonal Antibody (Clone Pri 308) IHC, WB Ha, Mo, Sh, H; (-) Bo

189720 Prion Protein Monoclonal Antibody (Clone SAF 32) ELISA, FC, IHC, WB H, Ha, Mo, Bo, Sh

189730 Prion Protein Monoclonal Antibody (Clone SAF 53) ELISA, FC H, Mo, Ha; (-) Bo, Sh

189740 Prion Protein Monoclonal Antibody (Clone SAF 54) IHC H, Ha, Bo, Sh, Mo

189755 Prion Protein Monoclonal Antibody (Clone SAF 61) ELISA, FC H, Ha, Bo, Sh, Mo

189765 Prion Protein Monoclonal Antibody (Clone SAF 83) ELISA, FC, WB Ha, Mo; (-) H, Bo, Sh

189775 Prion Protein Monoclonal Antibody (Clone SAF 84) IHC, WB Ha, Bo, Sh, Mo; (-) H

11866 Prion Protein Monoclonal Antibody (Clone SHA31) ELISA, WB not available

189770 Prion Protein Monoclonal Antibody (SAF-70) WB Ha, Mo, Bo, Sh, H

OptineurinItem No. Item Name Applications Species Reactivity/Specificity

100000 Optineurin (C-Term) Polyclonal Antibody WB H, Mo, R

100002 Optineurin (INT) Polyclonal Antibody WB not available



Cellular and Scrapie Forms of Prion ProteinPrion diseases include bovine spongiform encephalopathy

(BSE), scrapie of sheep and goats, chronic wasting disease

(CWD) of cervids, and human Creutzfeld Jakob disease

(CJD). Infectious transmission involves conversion of the

prion protein (PrP) from the cellular form PrPC to the disease-

associated scrapie prion, PrPSc. While PrPC is monomeric

and sensitive to proteinase K treatment in the presence

of detergents, PrPSc is prone to aggregation and partially

proteinase K resistant.

PrPC, encoded by the PRNP gene, undergoes

posttranslational modifications and is expressed as a

cell-surface glycosylated, GPI-anchored protein. The

C-terminal domain of PrPC forms a globular structure with

three α-helices (HI, H2, and H3) and two short anti-parallel

β-strands (S1 and S2), whereas the N-terminal domain is

highly flexible and lacks identifiable secondary structure.

H1 is an unusually hydrophilic α-helix located between

S1 and S2 with few contacts with the remainder of the

molecule, while H2 and H3 are hydrophobic, arranged anti-parallel and linked by a single disulfide bond. PrPC is composed of 42%

α-helix and 3% β-sheet while PrPSc contains 30% α-helix and 43% β-sheet. It is not clear whether PrPSc is produced from either mature PrPC

or an unfolded, intermediate isoform. Both conformations have potential to convert prion protein to a β-sheet-rich structure. Acting as a

seed, the β-sheet-rich conformation has a propensity to recruit additional β-sheet monomers to construct the irreversible, disease-related

isoform. As PrPSc monomers accumulate, the growing PrPSc polymers may break off into smaller fragments, which would enable further

dispersion of the pathogenic proteins and accelerate conversion of PrPC.

H1

H3S2

S1

S2S1

OR

signalpeptide

22 23 51 90

GPI anchorsignal

1 231 254

H1 H2 H3

Conversion

PrPC PrPSc

H2

S-S

PrPC protein (upper) and structural basis for PrPC to PrPSc conversion (lower)


Notes:

antibodies for neuroscience research - vinci …...10224 gpr55 polyclonal antibody fc, icc, wb bo,...

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