animal breeding and national animal products from …...ed f l uor es c enc e 100 90 80 70 60 50 40...

33
"Animal breeding and national animal products from a genomic point of view" Anagnostis Argiriou Researcher Institute of Applied Biosciences – CERTH http://inab.certh.gr

Upload: others

Post on 24-Jul-2020

2 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

"Animal breeding and national animal products from a genomic point of view"

Anagnostis ArgiriouResearcherInstitute of Applied Biosciences – CERTH

http://inab.certh.gr

Page 2: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Innovation drivers

Environmental•

Economic

Social•

Technology

Policy

http://inab.certh.gr

Page 3: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Genetics-

Genomics

http://inab.certh.gr

Page 4: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Cost of Sequencing

2/4/2015 http://inab.certh.gr

Page 5: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Sequenced Genomes

http://inab.certh.gr

Page 6: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

ΚΑΛΟΣ

ΚΑΚΟΣΩΡΑΙΑ

ΠΑΝΩΡΑΙΑ

Καλος

M M M

M M M

Καλoς

Page 7: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

METAGENOMICS

http://inab.certh.gr

Page 8: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Gut Microbiome

http://inab.certh.gr

Page 9: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Animal Breeding and Genomics Centers

Can I genetically select dairy cows based on their emission of methane?

Is it possible by measuring gene activity to find out whether chicken have good gut health?

How do we store genetic material for future generations best?

http://inab.certh.gr

Page 10: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Single Nucleotide Polymorphisms (SNPs) Microarrays

Bovine and Ovine Genotyping BeadChip•The BovineLD BeadChip enables accurate genotyping to understand the impact of genetics on milk production, reproduction, health•80,000 custom markers for bovine•42,000 custom markers for ovine

http://inab.certh.gr

Page 11: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Agri-Food Chain

Agri-SuppliesAgri-Supplies Primary production

Primary production TransformationTransformation TradeTrade ConsumptionConsumption

SeedsFertilizersPesticidesBreedsGenetic improvement

SeedsFertilizersPesticidesBreedsGenetic improvement

Agriculture methods

TechnologiesPrecise agriculture

Agriculture methodsTechnologiesPrecise agriculture

HarvestingTransformatio

n

ConservationPackagingEnergy consumption

WasteQuality controlTraceabilty

HarvestingTransformatio

nConservationPackagingEnergy consumptionWasteQuality controlTraceabilty

Marketing services

BrandingLogisticsTraceability

Marketing servicesBrandingLogisticsTraceability

Consumer needs

TourismRestaurationQuality controlTraceability

Consumer needsTourismRestaurationQuality controlTraceability

http://inab.certh.gr

Page 12: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Domestication of Animals

Domestication was done in different areas in different periods

First time in Mesopotamia (11.000 b.p.)

First domesticated species: sheep and goats (9.000 -11.000 b.p.)

Followed pigs (8.500 – 10.500 b.p.) and cattle

(8.000 -10.000 b.p.).

http://inab.certh.gr

Page 13: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Expansion of Animal domestication

http://inab.certh.gr

Page 14: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Animals in the Neolithic period in Greece

Τaurus primegenius ή

Bos primigenius

http://inab.certh.gr

Page 15: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

The Animal Husbandry in Bronze and Iron Age

http://inab.certh.gr

Page 16: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

http://inab.certh.gr

Autochthonous Animals in GreeceAutochthonous Animals in Greece

Page 17: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Autochthonous Animals

http://inab.certh.gr

Species Registered/referred racesCattle 36Buffalo 1Chicken 13Donkeys 4Goats 39Horses 20Pigs 6Sheep 46

Page 18: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

What happened the last 60 year in Greece

http://inab.certh.gr

Page 19: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

http://inab.certh.gr

Page 20: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

http://inab.certh.gr

Page 21: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

http://inab.certh.gr

Page 22: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

The Example of Greek Black Pig

Michailidou et al Genet. Mol. Res. 13 (2): 2752-2765 (2014)

Page 23: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Greek Black Pig

http://inab.certh.gr

Page 24: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Promotion

http://inab.certh.gr

Page 25: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

MC1R (melanocortin receptor type 1) -

1

deg.89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0

Nor

mal

ised

Flu

ores

cenc

e

100

90

80

70

60

50

40

30

20

10

deg.89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0

Nor

mal

ised

Flu

ores

cenc

e

100

90

80

70

60

50

40

30

20

10

White Pigs

Black PigsHybrid Pigs

Pata Negra Bellota

150 Euros/Kg

San Daniele

30 Euros/Kg

http://inab.certh.gr

Page 26: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

DNA Traceability

http://inab.certh.gr

Page 27: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

DNA certified products of animal and plant origin –

Unique «DNA signature»

Protection form adulteration and fraudulent actions

Could protect the added valued of PDO and PGI products –

Protection of the investment made from the stakeholders

Some Examples: Mozzarela di buffala, Feta Cheese, Greek Black Pig, Fava Santorinis etc.

http://inab.certh.gr

Page 28: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Greek Feta Cheese

+

Only Sheep and Goat milk

+ Max goat milk to 30%

-

It is prohibited to manufacture Feta Cheese

from other type of milk.

http://inab.certh.gr

Page 29: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

high-throughput sequencing technologies

to certify cheeses

http://inab.certh.gr

Page 30: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Microbial diversity of traditional cheeses

68 genera237 species

79%10 genera35 species

13%

14 genera21 species

8%

BacteriaYeast

Fungi

Livarot, Munster… (Surface)

Ref: MC Montel, ANR GRAMME 2008‐2010

St-NectaireAcinetobacter johnsoniiAlcaligenes Brevundimonas diminutaCitrobacterEnterobacter hormaecheiEscherichia coliHafnia alvei

Halomonas

Klebsiella oxytoca

Kluyvera intermediaMarinomonas sp. nov.

Morganella Proteus Providencia

Pseudoalteromonas

Pseudomonas.

Psychrobacter

Raoultella planticolaSerratiaTetrathiobacter kashmirensisVibrio litoralis

23%

17%

Acinetobacter

Chryseobacterium

Citrobacter freundii

Enterobacter

Klebsiella

Ochrobacterum sp.Pantoea agglomerans

Pseudomonas

Serratia rubideae

Sphingobacterium

Stenotrophomonas

Raoultella planticola

http://inab.certh.gr

Page 31: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Greek PDO Cheeses

http://inab.certh.gr

Page 32: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

http://inab.certh.gr

Page 33: Animal breeding and national animal products from …...ed F l uor es c enc e 100 90 80 70 60 50 40 30 20 10 deg. 89.5 90.0 90.5 91.0 91.5 92.0 92.5 93.0 93.5 94.0 94.5 95.0 N o r

Σας Ευχαριστώ

Δρ. Αργυρίου

ΑναγνώστηςΕρευνητής

Ινστιτούτου

Εφαρμοσμένων

Βιοεπιστημών

ΕΚΕΤΑΤηλ. 2310 498475Email: [email protected]

http://inab.certh.gr