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Anatomy of Bacteria
MorphologyStructureFunction
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Binary Fission
• “a method of asexual reproduction involving halving of the nucleus and cytoplasm of the cell followed by the development of each half into a new individual”
• septum• progeny cells• generation time
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Morphology
• Size
• Shape
• Arrangement
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Size
• prokaryotic (bacteria) cells are very small compared to eukaryotic cells
• prokaryotic cells are the most abundant form of life on earth
• prokaryotic cells can survive in conditions that are too extreme for eukaryotic cells
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Shape
• Readings question one:
What are the three basic shapes that most bacteria exhibit?
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Spiral(Vibrio, Spirillum, Spirochete)
• Vibrio: “curved or bent rods that resemble commas”
• Spirillum: “a corkscrew shape with a rigid cell wall and hair-like projections called flagella that assist in movement”
• Spirochete: “a flexible cell wall but no flagella in the traditional sense. Movement occurs by contractions (undulating) of long filaments (endoflagella) that run the length of the cell.”
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Arrangement
• Readings question two:What are the three basic arrangements that most bacteria exhibit?
Additional arrangements:Tetracocci: “grouping of four spherical shaped
cells”Sarcinae: “a cube-like packet of eight spherica
bacteria”
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Structure and Function• up until the 1950’s prokaryotes were believed to
simply be “bags of enzymes”• prokaryotes have a simpler construction than
eukaryotes• prokaryotic cell has 5 essential structural
components:– Nucleoid (bacterial chromosome)– Ribosomes– Cell membrane– Cell wall– Capsule
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Nucleoid
• bacterial chromosome• typically one large circular molecule of DNA• floats freely in the cytoplasm• genetic control center of the cell• determines all of the properties and functions
of the bacterium
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Ribosomes
• proteins and RNA• prokaryotic ribosomes are smaller than
eukaryotic ribosomes• protein synthesis• “granular” appearance
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Cell Membrane and Cell Wall
• Readings question three:
What is the difference between the cell membrane and the cell wall?
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Capsule
• “the membrane that surrounds some bacterial cells; a loose gel-like structure that, in pathogenic bacteria, helps to protect against phagocytosis”
• glycocalyx• slime layer
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Cytoplasm
• Readings question four:• What is the cytoplasm of prokaryotic cells?
• primary structures: nucleoid and ribosomes
• plasmids: extrachromosomal pieces of DNA
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Endospores
• “a thick-walled spore within a bacterium”
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Endospores
• produced by the bacterium to help it survive in an unfavorable environment
• formed by vegetative cells- “sporulation”• one of the most resistant forms of life
• germination
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Clostridium tetani
• deep wound punctures that become anoxic
• tetanus toxin spreads and causes disease
• spastic paralysis and can result in death
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Clostridium botulinum
• botulinum toxin in improperly preserved foods
• botulism can result in death due to respiratory failure as a result of muscle paralysis
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Clostrideium perfringens
• most prevalent reported cause of food poisoning
• enterotoxins in the intestines
• diarrhea and intestinal cramps with no fever or vomiting
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Flagella
• protein structures attached to the cell surface that resemble “whip-like” appendages
• distributed in distinguishing patterns
• flagella of prokaryotic cells differ from eukaryotic cells
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Pili (Fimbriae)
• short, hair-like structures on the surface of prokaryotic cells composed of protein
• shorter, thinner, and straighter than flagella• allow bacteria to attach to surfaces• e.g. Neisseria gonorrhoeae
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Gram-staining
• Readings question five:
• What is the purpose of gram-staining? What are the characteristics of gram-positive bacteria and gram-negative bacteria?
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Physiology of Bacteria
Bacterial Growth
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Bacterial Colony
• “a visible group of bacteria growing on a solid medium, presumably arising from a single microorganism”
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Requirements for Growth
• Physical: temperature, pH, light, osmotic pressure, moisture
• Chemical: carbon, nitrogen, sulfur, phosphorus, trace elements, oxygen
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Temperature
• Readings question three:
What are psychrophiles, mesophiles, and thermophiles?
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Psychrophiles
• Desulfofrigus oceanense (Arctic and Antarctic Oceans)
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Mesophiles
E. coli
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Thermophiles
Fossilized Microbes from Yellowstone’s Hot Springs
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Temperatures
• Minimum: “temperature below which bacterial growth will not take place”
• Optimum: “temperature at which organisms grow best”
• Maximum: “temperature above which bacterial growth will not take place”
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• What are the embalming implications associated with the temperature preference of bacteria?
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pH
• Readings question four:
Describe the pH scale.
Acidophiles: bacteria that are remarkably tolerant of acidity
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Light
• Cyanobacteria: oxygen producing prokaryotes– thrive in the presence of light
• Yeasts and Molds– prefer dark areas
• Some bacteria are destroyed by ultraviolet light.
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Osmotic Pressure
• “pressure that develops when two solutions of different concentrations are separated by a semi-permeable membrane”
• microorganisms require water for growth and are made up of 80-90% water
• high osmotic pressure removes necessary water from a cell
• plasmolysis• hypertonic solutions
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Moisture
• maximum, optimum and minimum requirement for all microorganisms
• Pathogenic bacteria are usually found in the body’s tissues
• Fungal diseases are usually found on the body surface.
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Chemical Requirements
• Readings question two:
Describe the differences between autotrophic bacteria and heterotrophic bacteria.
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Heterotrophic Bacteria
• 3 categories:– 1) strict (obligate) saprophyte– 2) strict (obligate) parasite– 3) facultative bacteria:• Facultative saprophyte: “prefers live organic matter as
a source of nutrition but can adapt to the use of dead organic matter under certain conditions”• Facultative parasite: capable of living and growing with
the nutrients that its host provides
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Carbon
• one of the most important requirements for microbial growth
• structural backbone of living matter• needed for all the organic compounds that
make up a living cell• ½ of the “dry weight” of a bacterial cell is
carbon
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Nitrogen, Sulfur, and Phosphorus
• needed by microorganisms for the synthesis of cellular material
• e.g. protein, DNA, RNA, ATP
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Trace Elements
• iron, copper, and zinc
• essential for the function of certain enzymes
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Oxygen
• Microbes that use molecular oxygen (aerobes) produce more energy from nutrients than microbes that do not use oxygen (anaerobes)
• Reading question two:Describe the difference between obligate
aerobes and obligate anaerobes.
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Microaerophilic Organisms
• “a microorganism that requires very little free oxygen”
• only grow in oxygen concentrations that are lower than those in air
• require about 2 – 10% free oxygen
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Facultative Organisms
• Facultative Aerobes: “a microorganism that prefers an environment devoid of oxygen but has adapted so that it can live and grow in the presence of oxygen”
• Facultative Anaerobes: “a microorganism that prefers an oxygen environment but is capable of living and growing in its absence”– E.g. Bacillus anthracis, Corneybacterium
diphtheriae, Escherichia coli
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Aerotolerant Organisms
• can grow in the presence or absence of oxygen– e.g. Streptococcus pyogenes
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Microbial Associations
• normal flora (microbiota)• transient microbiota• symbiotic relationship: “organisms live in close
nutritional relationships; required by one or both members”– distinguished by the degree to which the host
organism is harmed
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Mutualism
• “a symbiotic relationship in which organisms of two different species live in close association to the mutual benefit of each”– e.g. E. coli in the human digestive tract
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Commensalism
• “the symbiotic relationship of two organisms of different species in which one gains some benefit such as protection or nourishment and the other is not harmed or benefited”– e.g. bacteria on skin surface; microorganisms
within the digestive tract
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Parasitism
• “an interactive relationship between two organisms in which one is harmed and the other benefits”
• many disease-causing bacteria are parasites• typically the host is macroscopic and the
parasite is microscopic• roundworms and flatworms are parasites that
are large multi-cellular organisms
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• Readings question five:
What is the synergistic effect?
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Antagonism
• “mutual opposition or contrary action. The inhibition of one microorganism by another.”
• Involves competition among microbes• normal microbiota protect the host against
colonization by potentially pathogenic microbes
• normal flora produce substances harmful to the invading microbes (pH, oxygen)