A trusted CRO to speed up your R&D programs

ANAQUANT ‘At a glance’

! Contract Research Organization

!  specialized in targeted and absolute protein quantification by Mass spectrometry,

!  offering customized services in assay development and samples bioanalysis.

!   Supports pharmaceutical and biotech companies to speed up their R&D programs with robust and validated analytical tools

!   ANAQUANT was founded by Tanguy Fortin in 2014 after more than ten years of research driven by Pr Jérôme Lemoine at the «Analytical Sciences Institute » of Lyon.

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A purpose for your laboratory

! simplify and accelerate the development of your bioanalytical tests

!   Quickly validate or unvalidate your scientific hypotheses with limited expenses and risk

! Cross validate your reference method

! Bring additional analytical information like precise measurement of proteins modifications.

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Whether you are concerned by upfront research, quality control, or clinical study, we propose you an  orthogonal  antibody-free alternative to immunoassay approach in order to:

3 offers to cover your needs

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Evaluation, validation and quantitation of

protein biomarker(s) tailored to your clinical

question

Absolute and sensitive quantification of

targeted protein and homologs in various biological matrices

Development of assay to contribute to Critical Quality Attribute the

definition of biotherapeutic and the bioprocess validation

Biomarker Targeted protein quantitation

Product Quality & Bioprocess validation

MULTIPLEXING SPECIFICITY ROBUSTNESS

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Biomarker

!   Single or multiplexed biomarker analysis (10 to 100 proteins)

! markerQUANT: a analytical tool developed with the Camille Jordan Institute, a worldwide renowned mathematic institute, to guarantee accurate and specific results.

! Discovery of biomarkers thanks to the fullQUANT approach

Main applications ! disease mechanism study

! i d e n t i f i c a t i o n o f n e w therapeutic targets

! early assessment of drug efficacy or safety

! identification of your targets in body fluids

! development of companion diagnostic test…

! targetQUANT: precise and absolute quantification of targeted protein with or without post-translational modifications.

!   Based on innovative technology to ensure specificity and robustness

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Targeted protein quantification

Main applications ! Level of protein target-ligand

! Early PK/PD assessment

! Measure of post-translational modification (PTM)

! Cross validation with reference method

! Transgene expression for gene therapy drug

! One analysis instead of several immunoassay kits

!   Services based on EMA guidelines related to biotherapeutics (protein, Ab, gene therapy, vaccines)

!   Goals: to contribute to the Critical Quality Attribute evaluation of biotherapeutics  & the bioprocess validation. 

! Antibody free analysis to speed up your bioprocess evaluation

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Product quality & bioprocess validation

Main applications ! Identity test for Ab, proteins,

vaccines

! Specific proteins degradation pathways

! Potency tests

! Low levels of impurities (HCPs)

! Biosimilar comparability study

! Bioprocess scale-up comparability study

Our know-know at your services !   Regarding your challenge, your concern, ANAQUANT answers with

customized services.

!   Milestones with Go/NoGo decision are established for each project to secure your budget.

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Assay development Assay validation

Technology transfer

Sample bioanalysis

Feasibility study

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Main advantages of our technology

No antibody

Multiplexing

Absolute quantification

Short assay development time

Minimal sample volume

Biological complex matrices analysis (serum, plasma, urine, tissue)

The added value of our scientific expertise

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Advices on the protein assay to implement

Innovative approaches which improve robustness &

sensitivity of MS analysis

Validated protocols to control pre-analytical factors

& biological samples preparation

Method and experience in data interpretation

Access to:

Main publications !   Clinical quantitation of prostate specific antigen biomarker in the low nanogram/mL range by

conventional bore liquid chromatography-tandem mass spectrometry (MRM) coupling and correlation with ELISA tests Fortin T, Salvador A, Charrier JP, Lenz C, Lacoux X, Morla A, Choquet-Kastylevsky G, Lemoine J. - Mol Cell Proteomics. 2009

!   Multiple reaction monitoring cubed for protein quantification at the low nanogram/milliliter level in nondepleted human serum. Fortin T, Salvador A, Charrier JP, Lenz C, Bettsworth F, Lacoux X, Choquet-Kastylevsky G, Lemoine J. - Anal Chem, 2009

!   The current status of clinical proteomics and the use of MRM and MRM(3) for biomarker validation Jérôme Lemoine, Tanguy Fortin, Arnaud Salvador, Aurore Jaffuel, Jean-Philippe Charrier, Geneviève Choquet-Kastylevsky - Expert Review of Molecular Diagnostics

! Absolute quantification of podocin, a potential biomarker of glomerular injury in human urine, by liquid chromatography-multiple reaction monitoring cubed mass spectrometry. Simon R, Lemoine J, Fonbonne C, Jaffuel A, Léonard JF, Gautier JC, Pasquier O, Salvador A. - J Pharm Biomed Anal. 2014

To  discuss  about  your  project  please  contact  us:    

Tanguy.fortin@anaquant.com www.anaquant.com