Analytical & Biological Mass Spectrometry Facility

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The Analytical & Biological Mass Spectrometry Facility has two locations where researchers can bring samples for analysis. There, facility personnel will determine the mass of the molecular components of a sample, for structural confirmation and/or identification. The Old Chemistry Building lab focuses on confirmation of synthetic products, measurement of polymers and whole proteins, and assistance with determination of unknowns. In the Keating location, work most often involves characterization of biological protein, lipid and metabolite samples, in either pure or complex form.

The Facility is equipped with mass spectrometers that can measure molecular weights very accurately, including a Bruker 9.4 Tesla FT-ICR, a Thermo LTQ Orbitrap Velos, and a Thermo Q Exactive Orbitrap. The latter two instruments are dedicated to the analyzing of peptides from proteins. The Facility also has a second Q Exactive Orbitrap that is dedicated to the analysis of lipids and metabolites. Very high mass measurements of whole proteins and polymers can be made using the Facility’s Bruker Autoflex Speed MALDI-TOF and Ultraflex III MALDI-TOF-TOF. Routine analyses of a variety of compounds are made using the Bruker AmaZon SL Ion Trap, the ABI Sciex 4000 Q-Trap and the Shimadzu GC-MS. Additional equipment in the Facility include a Biacore T100 SPR Biosensor to measure biomolecular interactions, a UV-HPLC, a FPLC, and 1D- and 2D-gel electrophoresis equipment.

The Facility regularly works with researchers in the UA’s College of Science, College of Medicine, and College of Agriculture and Life Sciences, as well as a variety of product-producing industry partners in biotechnology, biomedicine, and more.

Analytical & Biological Mass Spectrometry Facility

Engage with us todayContacts Locations

Linda Breci Staff Scientist breci@email.arizona.edu 520-626-4108

George Tsaprailis Staff Scientist georget@email.arizona.edu 520-626-5461

Thomas W. Keating, BIO5 Building 240 Room 106

Old Chemistry Building 41 Room 112