Analyte Quarterly 2012Q4

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The broadest selection of analytes acrossa wide range of disease states, including metabolic disease, inflammation, neurodegenerative disease, toxicity,cancer and more. All the components and reagents you need to detect multiple analytes simultaneously in a small sample size (10-50 L)all in a single kit, using a single catalogue number.

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<p>Immunoassays &amp; MILLIPLEX map KitsThe #1 Best Seller for Multiplexing using Luminex technology</p> <p>Analyte Quarterly Q4 2012 Bone </p> <p>Metabolism Disease</p> <p>Cancer Cell Signaling Metabolism</p> <p> Cardiovascular </p> <p> Cellular</p> <p> Inflammation/Immunology </p> <p>Metabolism Stem Cell Characterization</p> <p> Neuroscience </p> <p> Toxicity</p> <p>EMD Millipore is a division of Merck KGaA, Darmstadt, Germany</p> <p>The Latest in Multiplexing &amp; ELISAsDiscover the Latest in Innovative and Unique Assay Kits, Instruments and ServicesMILLIPLEX map Multiplex Immunoassay KitsMagnetic Bead FormatKit Cancer/Metastasis Panel Biomarker 1 Early Apoptosis Signaling - 7 Plex Kidney Toxicity Panel 1 Late Apoptosis Signaling - 3 Plex MAPK/SAPK Signaling - 10 Plex Neurodegenerative Disease Panel 2 Cytokine/Chemokine Cytokine/Chemokine - 13 Plex Premix Soluble Cytokine Receptor Src Family Kinase Active Site - 8 Plex Stem Cell Pluripotency Panel 1 T-Cell Receptor - 7 Plex Th17 - 25 Plex Premix Human Human Human Porcine Porcine Mouse Canine Human Species Human Catalogue No. HCMBMAG-22K 48-669MAG CKTX1MAG-97K 48-670MAG 48-660MAG HNDG2MAG-36K PCYTMAG-23K PCYTMG-23K-13PX MSCRMAG-42K 48-650MAG 48-617MAG 48-690MAG HT17MG-14K-PX25</p> <p>NEW PA NEL S</p> <p>ELISA Kits Cytokine ELISAs FGF-23 (Rat / Mouse) - 10-pack Glucagon (Human / Rat / Mouse) Glucagon (Human / Rat / Mouse) - 10-pack GLP-1 HS (Active) (Multispecies) - 10-pack GLP-2 (Total) (Rat / Human) - 10-pack</p> <p>MAGPIX Instrument Multiplex analyte detection system (See page 21)</p> <p>Services Biomarker Services (See back cover)</p> <p>New MILLIPLEX Analyst 5.1 Software!You asked for new and enhanced features and we listened! Our new software provides you with the data you need, combining speed and accuracy, even at the lowest part of the curve. For details, see page 22.</p> <p>20% OFFMILLIPLEX Analyst 5.1 Software one- or five-seat licenses now through December 31, 2012(Use promo code ZBIOS584)</p> <p>NEW PRO DU CT SPOTLIG H T</p> <p>MILLIPLEX MAP &amp; ELISAs New Product SpotlightImmunology: MILLIPLEX MAP Human and Mouse(Cat. No. HTH17MAG-14K) (Cat. No. MTH17MAG-47K)</p> <p>Discover the most versatile assay platform for autoimmune disease and immunology research. T-cell differentiation and cytokine secretion regulate immune response, chronic inflammation, autoimmune diseases and cancer. Get the most information from each sample with our HumanMouse Plasma Cytokines600 Plasma Levels (pg/mL) 400 300 200 100 0 0 1 IL-13 2 Time after LPS (hr) IL-17F 4 IL-17A 8 Plasma Levels (pg/mL) 500 9000 8000 7000 6000 5000 4000 3000 2000 1000 0</p> <p>and Mouse Th17 panels that enable you to quantify signature cytokines from not only Th17 cells but, Th1 and Th2 cell subsets as well. With this broader analytical scope, you can expand each experiment beyond Th17 cytokines.Mouse Plasma Cytokines</p> <p>Figure 1A and 1B. Examination of cytokine response in mouse plasma at various time points after an in vivo LPS-challenge.8 IFN</p> <p>0 IL-6</p> <p>1 IL-22</p> <p>2 Time after LPS (hr) TNF</p> <p>4 IL-10</p> <p>Cell Signaling: MILLIPLEX MAP TGF Pathway 6-Plex Panel(Cat. No. 48-614MAG)</p> <p>Understanding regulation of the TGF signaling network requires simultaneous analysis of multiple, mutually interacting signaling proteins. The MILLIPLEX MAP TGF Pathway 6-Plex Panel, based on the Luminex xMAP technology, moves your research faster by enabling you to quantify multiple Smads and other TGF pathway proteins simultaneously. Use Iess sample. Get more data. Better data than you ever got from Western blotting! Advantages of MILLIPLEX MAP Cell Signaling Kits over traditional cell signaling assays:4000 4000 3000MFI</p> <p>Analyze dozens of samples using a 96-well plate</p> <p> Analyze time course or dose response curves, alI in one assay Measure concentrations of multiple total or phosphorylated proteins in the same well Use median fluorescence intensity to measure fold increases comparing stimulated and unstimulated cell cultures or tissue lysates Validated using Western blotting and IPFigure 2A and 2B. TGF pathway proteins were simultaneously detected in two sets of human tissue samples (20 g/well). PhosphoSmad2 was significantly elevated in breast cancer tissue samples.</p> <p>Breast Normal 2 Breast Cancer 2</p> <p>Breast Normal 1 Breast Cancer 1 MFI</p> <p>3000</p> <p>2000</p> <p>2000 1000 0</p> <p>For Research Use Only.</p> <p>1000</p> <p>TGF2</p> <p>pERK</p> <p>pSmad2</p> <p>pAkt</p> <p>pSmad3</p> <p>Smad4</p> <p>0</p> <p>TGF2</p> <p>pERK</p> <p>pSmad2</p> <p>pAkt</p> <p>pSmad3</p> <p>Smad4</p> <p>emdmillipore.com/analytes_q41</p> <p>NEW PRO DUCT SPOTLIGH T</p> <p>Toxicity: MILLIPLEX MAP Rat Kidney Toxicity Magnetic Bead Panel 1(Cat. No. RKTX1MAG-37K)</p> <p>Toxicity is the leading cause of drug failure, so increasing the success rate of therapeutic development requires more sensitive and rapid methods to determine organ-specific damage. The traditional tests to determine kidney toxicity are blood urea nitrogen (BUN) and serum creatinine (SCr), which are not optimal for early detection or localization of kidney damage. As a result, researchers have turned to recently qualified nephrotoxicity biomarkers for more predictive toxicity analysis. We have developed the MILLIPLEX MAP Rat Kidney Toxicity MagneticClusterin1000 900 800 700 600 500 400 300 200 100 0 H2O low dose gentamicin high dose gentamicin ng/mL 10.0 9.0 8.0 7.0 6.0 5.0 4.0 3.0 2.0 1.0 0.0</p> <p>Bead Panels 1 and 2, containing a total of 14 kidney toxicity biomarkers. We used these panels to efficiently profile response to gentamicin, a known nephrotoxicant in rats. By simply measuring biomarker concentrations in urine, we detected kidney toxicity (marked by increased Clusterin and KIM-1 levels) in as few as three days (Figure 3).Figure 3A and 3B. 3 groups of 5 adult male Sprague Dawley rats, each injected intraperitoneally daily with either water, low-dose (70 mg/kg) gentamicin or high-dose (140 mg/kg) gentamicin. Day 0 urine collection is after the 1st dose, and subsequent collections are at days 3, 7 and 14. All KIM-1 nephrotoxicity biomarkers H2O were analyzed, but we low dose gentamicin show data here only for high dose gentamicin Clusterin and KIM-1. Analyte concentrations in gentamicin treated animals spike at day 3 for both Clusterin and KIM-1, and decrease at days 7 Day 3 Day 7 Day 14 and 14.</p> <p>ng/mL</p> <p>Day 0</p> <p>Day 3</p> <p>Day 7</p> <p>Day 14</p> <p>Day 0</p> <p>Metabolism: ELISA - Circulating Glucagon(Cat. No. EZGLU-30K) (Cat. No. EZGLU-30BK)</p> <p>Glucagon is the most important counterregulatory hormone to insulin in blood glucose homeostasis. It stimulates gluconeogenesis and glycogenolysis while inhibiting glycogenesis and glycolysis mainly in the liver. Recently, glucagon and its receptors have been actively pursued as therapeutic targets for Type I diabetes. However, quantifying glucagon in blood has been challenging due to glucagons many immunoreactive forms.100 10 4 Sample Results Using ACN Extraction, pg/ml 80</p> <p>EMD Millipores new, highly specific glucagon ELISA kit shows no cross-reactivity to miniglucagon or glucagon 118 and &lt; 5% cross-reactivity to oxyntomodulin. This assay, including sample extraction using our rapid protocol (Figure 4B), can be completed within a working day. In contrast to kits from competing suppliers, results from our ELISA kit agree well with published glucagon levels.Figure 4. A. EMD Millipores Glucagon ELISA (Cat. No. EZGLU-30K) kit exhibits sensitive, precise detection of full length, porcine glucagon with low interassay variation. B. Correlation of glucagon measurements following sample extraction using rapid acetonitrile extraction (y-axis) with measurements following extraction using solid phase extraction (x-axis).</p> <p>RLU (Mean S.D., n=5)</p> <p>60</p> <p>10 3</p> <p>40</p> <p>For Research Use Only.</p> <p>Y = 1.024(x) + 2.06 r = 0.903 n = 21</p> <p>10 2 0.01 0.1 1 Porcine Glucagon, ng/ml Standard Curve Assay Background</p> <p>20</p> <p>0</p> <p>0</p> <p>20 40 60 80 100 Sample Results Using Waters HLB Extraction Plate, pg/ml</p> <p>2</p> <p>ELISAs &amp; RIAsEMD Millipore provides an unparalleled level of peer supportscientists working with scientiststo provide you with the quality tools and services you need to do your best work. With a wide range of ELISAs and RIAs, we advance your research in many research areas including metabolic disease (diabetes, obesity, cardiovascular), inflammation, immunology, neuroscience, oncology and cell signaling. Plus, some of our most popular ELISAs are available a 10-pack format. ELI S A / RI A</p> <p>*Cytokine ELISAsKit IL-2 IL-2 IL-4 IL-4 IL-6 IL-6 IL-10 IL-10 TNFa TNFa Species Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Catalogue No.NEW! NEW! NEW! NEW! NEW! NEW! NEW! NEW! NEW! NEW!</p> <p>Neuroscience ELISAs, continuedKit Neuropeptide Y (NPY) Neuropeptide Y (NPY) Phosphorylated Neurofilament (pNF-H) Pigment Epithelium Derived Factor (ChemiKine) S100B SAA-3 Species Human Rat/Mouse Multispecies Human Catalogue No. EZHNPY-25K EZRMNPY-27K NS170 CYT420 EZHIL2 EZMIL2 EZHIL4 EZMIL4 EZHIL6 EZMIL6 EZHIL10 EZMIL10 EZHTNFA EZMTNFA</p> <p>Human Mouse</p> <p>EZHS100B-33K EZMSAA3-12K</p> <p>ELISA Circulating BiomarkersKit Adiponectin 10 - Pack Species Human Mouse Rat Canine Human Catalogue No. EZHADP-61K EZHADP-61BK EZMADP-60K EZMADP-60BK EZRADP-62K EZCADP63K EZHMWA-64K EZHMWA-64BK Mouse Human Human Human Human/Rat Human Human Rat/Mouse Human Rat Canine Human Rat/Mouse EZMADPSN-22 EZHA-52K EZHA-52BK EZHAT-51K EZHAPOA4-73K CYT306 EZHCMRN-57K EZHCP-20K EZHCP-20BK EZRMCP2-21K CYT298 CYT294For Research Use Only.</p> <p>* 10-Pack Format Now Available for Select Kits!</p> <p>Neuroscience ELISAsKit a-Synuclein Amyloid Beta, 1-40 Amyloid Beta, 1-42 Amyloid Beta, SET Amyloid Beta (Brain), 1-40 Amyloid Beta (Brain), 1-42 Amyloid Beta (Brain), SET Brain Derived Neurotrophic Factor (ChemiKine) Glial fibrillary acidic protein (GFAP) Nerve Growth Factor (ChemiKine) Neuropeptide S (NPS) Species Human/Rat/ Mouse Human Human Human Human Human Human Human/Rat Catalogue No. NS400 EZHS40 EZHS42 EZHS-SET EZBRAIN40 EZBRAIN42 EZBRAIN-SET CYT306</p> <p>Adiponectin 10 - Pack Adiponectin Adiponectin Adiponectin High Molecular Weight 10 - Pack Adipsin Amylin (active) 10 - Pack Amylin (total) Apo AIV BDNF Chemerin C-Peptide 10 - Pack C-Peptide 2 CRP CRP CRP FGF-21 FGF-21</p> <p>Human/Rat/ Mouse Rat/Mouse Human</p> <p>NS830 CYT304 EZHNPS-34K</p> <p>CYT296 EZHFGF21-19K EZRMFGF21-26K</p> <p>emdmillipore.com/analytes_q43</p> <p>ELISA Circulating Biomarkers, continuedKit FGF-23 10 - Pack FGF-23 10 - Pack FGF-23 10 - Pack Species Human RatNEW!</p> <p>Catalogue No. EZHFGF23-32K EZHFGF23-32BK EZRFGF23-42K EZRFGF23-42BK</p> <p>Kit Procollagen Type IIA N-Propeptide (PIIANP) Proinsulin (Intact) Proinsulin (total) 10 - Pack PYY (total) 10 - Pack RBP4 Resistin 10 - Pack</p> <p>Species Human Human Human Human Human Human</p> <p>Catalogue No. EZPIIANP-53K EZHIPI-17K EZHPI-15K EZHPI-15BK EZHPYYT66K EZHPYYT-66BK EZHRBP4-18K EZHR-95K EZHR-95BK</p> <p>Mouse Human Rat/Mouse Human Rat Human Rat/Mouse Multispecies Multispecies</p> <p>EZMFGF23-43K NEW! EZMFGF23-43BK EZGRA-88K EZGRA-88BK EZRGRA-90K EZGRT-89K EZGRT-89BK EZRGRT-91K EZHGIP-54K EZHGIP-54BK EZRMGIP-55K EZRMGIP-55BK EGLP-35K EGLP-35BK EZGLPHS-35KNEW!</p> <p>ELI S A / RI A</p> <p>Ghrelin (active) 10 - Pack Ghrelin (active) Ghrelin (total) 10 - Pack Ghrelin (total) GIP (total) 10 - Pack GIP (total) 10 - Pack GLP-1 (active) 10 - Pack GLP-1 High Sensitivity (active) 10 - Pack GLP-1 (total) 10 - Pack GLP-2 (total) 10 - Pack Glucagon 10 - Pack Growth Hormone 10 - Pack IFNa Insulin 10 - Pack Insulin 10 - Pack Laminin Leptin Dual Range 10 - Pack Leptin 10 - Pack Leptin 10 - Pack Leptin Nerve Growth Factor (ChemiKine) Omentin-1 Pancreatic Polypeptide 10 - Pack PEDF (ChemiKine)For Research Use Only.</p> <p>ELISA Other AssaysEZ-Transcription FactorDescription AP-1 Family c-Fos c-Jun ErbB2 (Tyr1248) HDAC Jun/Fos NFkB Family NFkB p50 NFkB p50/p65 NFkB p65 PI3 Kinase Activity/Inhibitor PhosphaFluor HTS OmniPhosphatase Ras Activation ssDNA Apoptosis Universal TF Colorimetric Catalogue No. 70-550 70-545 70-540 17-490 17-374 70-546 70-560 70-515 70-510 70-520 17-493 17-448 17-497 APT225 70-501 70-601 70-646 70-660 70-615 70-610 70-620 Chemiluminescent Catalogue No. 70-650 70-645 70-640</p> <p>EZGLPHS-35K EZGLP1T-36K EZGLP1T-36BK</p> <p>Multispecies Human/RatNEW!</p> <p>EZGLP2-37K EZGLP2-37BK EZGLU-30K EZGLU-30BK EZRMGH-45K EZRMGH-45BK Cyt271 EZHI-14K EZHI-14BK EZRMI-13K EZRMI-13BK EZHIASF-14K ECM310 EZHL-80SK EZHL-80BK EZML-82K EZML-82BK EZRL-83K EZRL-83BK EZCL-31K CYT304</p> <p>Human/Rat/ Mouse Rat/Mouse Human Human Rat/Mouse</p> <p>NEW! NEW!</p> <p>Insulin (Animal Serum Free) Human Human Human Mouse Rat Canine Rat/Mouse Human Human Human</p> <p>ELISA Other AssaysExtracellular MatrixKit Fibronectin sCD26 sE-Selectin sICAM-1 sICAM-2 sICAM-3 sL-Selectin sVCAM-1 Species Human Human Human Human Human Human Human Human Catalogue No. ECM300 ECM345 ECM330 ECM335 ECM346 ECM337 ECM331 ECM340</p> <p>EZH0MNTN1-29K EZHPP40K EZHPP-40BK CYT420</p> <p>* </p> <p>Coming Soon Solid Plate Format CU.S. Patent #: 5,541,066: 5,780,240 Requires Sample Extraction</p> <p>4</p> <p>ELISA Cell Signaling / IntracellularStar ELISAsDescription AKT1 (total) EGFR (total) Erk 1/2 (total) FAK (total) Insulin Receptor ( subunit) (total) IkB (total) JNK 1/2 (total) MEK1 (total) Met (total) p38a (total) PRAS40 (total) Src (total) phospho-Akt (Ser473) phospho-Akt (Thr308) phospho-EGFR (Tyr1068) phospho-EGFR (Tyr1173) phospho-FAK (Tyr397) phospho-IR ( Subunit) (Tyr 1162/1163) phospho-IRS1 (Ser307 mouse/Ser312 Human) phospho-IkB (Ser32) phospho-JNK 1/2 (Thr183/Tyr185) phospho-MEK1 (Ser218/222) phospho-MET (Tyr1230/Tyr1234/Tyr1235) phospho-p38a (Thr180/Tyr182) phospho-p53 (Ser15) phospho-PRAS40 (Thr246) Catalogue No. 17-455 17-460 17-463 17-479 17-483 17-485 17-465 17-473 17-469 17-487 17-477 17-467 17-457 17-456 17-462 17-461 17-480 17-484 17-459 17-486 17-466 17-474 17-470 17-488 17-475 17-478</p> <p>continuedKit</p> <p>Formerly EMD Chemicals ELISAs,Species Human Human Human Multi-species Human Human Human Human Human Human Mouse Human Catalogue No. QIA96 QIA61 QIA54 QIA40 QIA99 QIA52 QIA23 QIA29 CBA053 CBA071 QIA10 PMN Elastase/a1-PI Complex TGFa TIMP-1 TIMP-2 Soluble t-PA VEGF Total Bcl-2 Cathepsin D (Rapid Format) COX-2 Total CREB Total c-ErbB2/c-Neu (Rapid Format) PhosphoDetect ERK1/2 (Thr185/Tyr187)</p> <p>ELIS A / RIA</p> <p>Human Rat Mouse</p> <p>CBA006</p> <p>For technical support and ordering, visit: www.emdmillipore.com/chemicals</p> <p>RIAKit Adiponectin Adiponectin C-Peptide C-Peptide C-Peptide C-Peptide Ghrelin (active) Ghrelin (total) GLP-1 (active) GLP-1 (total) Glucagon Insulin Insulin Insulin Lispro Insulin Specific Insulin Ultra Sensitive Insulin Leptin Leptin Leptin Leptin Proinsulin PYY PYY (3-36) Specific PYY (total) Human Human Rat Human Mouse Rat Multi-species Human Rat/Mouse Human Human Species Human Mouse Human Rat Canine Porcine Human Human Multi-species Multi-species Multi-species Rat Porcine Catalogue No. HADP-61HK MADP-60HK HCP-20K RCP-21K CCP-24HK PCP-22K GHRA-88HK GHRT-89HK GLP1A-35HK GLP1T-36HK GL-32K RI-13K PI-12K LPI-16K HI-14K HI-11K SRI-13K HL-81K ML-82K RL-83K XL-85KFor Research Use Only.</p> <p>ELISA Cell-based AssaysDual Detect CELISAsDescription ErbB2 (Tyr1248) H2A.X (S...</p>

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