ampliqon pcr enzymes for dna amplification

Ampliqon PCR Enzymes for DNA Amplification

Ampliqon page 2

CUSTOM-MADEAmpliqon was founded as a small-scale production company in 2002.

Our basis is custom-made products for lifescience research and industries in Denmarkand abroad. As a consequence our presentproduct line comprises product combina-tions originally introduced by Ampliqon aswell as products not offered anywhere else.

EXAMPLES ARE:

GLYCEROL-FREE TAQdeveloped for the North American market

AMPLIQ GENOMIC AMPLIFIERdeveloped for Statens Serum Institut (The Danish Serum Institute)

HOT START MASTER MIX BLUEdeveloped for the European market

Today, we are a well consolidated companywith a loyal and growing customer networkand a highly skilled laboratory staff.

We focus on two consistent ideals: flexibility and service.

We welcome individual customer requests,offer prompt product and technical supportand meet any enquiry with swift attention.

SMALL-SCALE AND SMALL BATCHWe base our procedures on small batchproduction. This way we stay in control ofthe enzyme purification process and ensurethe same quality of each product.

Ampliqon PCR enzymes are used in a multitude of settings to make DNA copiesthousands of times. Therefore, our respon-sibility is to offer PCR enzymes that youcan trust for reliable and successful DNAamplification.

THIS CATALOQUEThe purpose of this catalogue is to offeryou a handy overview of our PCR productline.

The catalogue is supplemented by a separate price list.

We hope that our catalogue conveys thatwe are proud to be your supplier of highquality PCR enzymes.

Kind regards

AMPLIQON A/S

A Danish manufacturer of bioreagents and PCR enzymes

DEAR CUSTOMER

Why buy Ampliqon PCR enzymes?

Ampliqon page 2

TEXT Birgitte Vedelstorp Andersen LAYOUT [ di’zain ] Anne Mette Jensen IMPRINT Ampliqon A/S, Skovlunde PRINTER’S FROM grafisk as, Kolding Denmark 20102nd impression

Ampliqon

WHOLE GENOME AMPLIFICATION

4 AmpliQ Genomic Amplifier

PCR AMPLIFICATION

HOT START

6 TEMPase Hot Start DNA Polymerase

8 TEMPase Hot Start Master Mix

9 TEMPase Hot Start Master Mix Blue

REAL TIME

10 RealQ PCR Master Mix

HIGH FIDELITY

11 AccuPOL DNA Polymerase

TAQ

12 Taq DNA Polymerase

15 Taq RedPOL DNA Polymerase

16 Taq DNA Polymerase Master Mix

18 Taq DNA Polymerase Master Mix Red

NUCLEOTIDES

19 dNTP Mix

19 dNTP Set

19 dATP, dCTP, dGTP and dTTP

ISOTHERMAL AMPLIFICATION

AMPLIFICATION AT ONE TEMPERATURE

21 OneTEMP custom-made

22 PRACTICAL INFORMATION

23 AMPLIQON BIOREAGENTS

Product catalogue

Ampliqon page 4

WHEN DO YOU USE AMPLIQ GENOMIC AMPLIFIER?If you have too little DNA to run a PCR. The AmpliQ Genomic Amplifier is a whole genomeamplification kit which generates an unlimited source of representative DNA from limitedsource material. All your DNA input is amplified. The AmpliQ Genomic Amplifier is suited fora variety of genetic studies such as genetic analysis, DNA storing, library construction andforensic work.

FEATURES

Ideal for single cell PCR

No additional primers needed

From pg template DNA to µg DNA in a 3-6 hour incubation at 30°C

DESCRIPTIONThe AmpliQ Genomic Amplifier exponential-ly amplifies single or double stranded linearDNA templates during an isothermal strand

displacement reaction, and the DNA gene-rated is of high molecular weight. A minorpart of the DNA will be single stranded – the major part double stranded.

Most DNA purification methods can beused to generate a starting template. Inmany cases unpurified cell lysates can bedirectly used as starting material.

The performance of AmpliQ Genomic Amplifier at isothermal temperature (30°C): illustrated by nine concentrations ofhuman genomic DNA ranging from 12.5 ng to 49 pg.

AMPLIQ GENOMIC AMPLIFIER

WHOLE GENOME AMPLIFICATION

AmpliQ Genomic Amplifier

AMPLIQ GENOMIC AMPLIFIER

PRODUCT NUMBER

280801 25 reactions

280803 100 reactions


Ampliqon page 4

Fluorescence (dR)60000

50000

40000

30000

20000

10000

0 0 60 60 120 180180 240 300300 360 420 420 480 540540 600 660660 720 780780 840 900900 960 10201020 1080 1140 1140 1200 1261260 Minuts

NTC (no template control) 12.5 ng gDNA 6.25 ng gDNA

3.13 ng gDNA 1.56 ng gDNA 781 pg gDNA 391 pg gDNA

195 pg gDNA 98 pg gDNA 49 pg gDNA

Ampliqon

WHEN DO YOU USE TEMPASE HOT START DNA POLYMERASE?TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplificationand to diminish the formation of non-specific priming events during set-up. TEMPase HotStart DNA Polymerase allows for detection of low target copy numbers and for multiplexPCR in a single reaction tube.

FEATURES

Reaction set-up at room temperature

INCREASED

Sensitivity

Specificity

Product yield

DESCRIPTIONTEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNAPolymerase, which is activated by heat treatment.

A chemical moiety is attached to the enzyme at the active site, which rendersthe enzyme inactive at room temperature.Thus, during set-up and the first ramp ofthermal cycling, the enzyme is not activeand misprimed primers are not extended.

Once the reaction reaches optimal activa-ting temperature, the chemical moiety iscleaved during a heat activation step, releasing the active TEMPase Hot StartDNA Polymerase into the reaction.

HOT START

TEMPase Hot Start DNA Polymerase

TEMPASE HOT START DNA POLYMERASE

PRODUCT NUMBER

Buffer I & II

220302 250 units

220303 500 units

220304 1000 units

220305 2000 units

220306 2500 units

220307 5000 units

220308 10000 units

220315 50000 units

Buffer II

232702 250 units

232703 500 units

232704 1000 units

232706 2500 units

Ampliqon page 6

Ampliqon

Ampliqon Competitor Q Competitor P Competitor I Competitor F Competitor FS

Chemical Antibody Antibody Chemical Genetic modified based hot modified hot modified hot modified hot

Taq start Taq start Taq start Taq start Taq

Comparison with and without hot start activation: Ampliqon TEMPase Hot Start DNA Polymerase and competitors.Amplification of a 210 bp fragment of the phenylalanine hydroxylase (PAH) gene conducted according to supplier’sinstructions.

Comparison with and without hot start activation: TEMPase Hot Start DNA Polymerase and competitor Q.Amplification has been conducted according to supplier’s instructions.

AMPLIFICATION WITH AND WITHOUT HOT START

Fluorescence (dRn)4.7

3.7

2.7

1.7

0.7

-0.31 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 Cycles

10 units TEMPase, 100 ng DNA, hot start 10 units TEMPase, 100 ng DNA, no hot start

5 units TEMPase, 100 ng DNA, hot start 5 units TEMPase, 100 ng DNA, no hot start

10 units competitor Q, 100 ng DNA, hot start 10 units competitor Q, 100 ng DNA, no hot start

5 units competitor Q, 100 ng DNA, hot start 5 units competitor Q, 100 ng DNA, no hot start

WHEN DO YOU USE TEMPASE HOT START MASTER MIX?TEMPase Hot Start 2 x Master Mix is a ready to use mix that offers easy reaction assemblyat room temperature prior to hot start PCR. Just add template and primers. TEMPase Hot Start 2 x Master Mix is an alternative to TEMPase Hot Start DNA Polymerase and isapplicable for primer extensions and other amplification purposes.

FEATURES

Minimal optimisation

Convenient reaction set-up

Fewer pipetting steps

Consistent test results

DESCRIPTIONAmpliqon TEMPase Hot Start DNAPolymerase, the NH4+ buffer system,dNTPs and magnesium chloride are present in TEMPase Hot Start 2 x MasterMix with TEMPase Buffer I or TEMPaseBuffer II.

The TEMPase buffer system covers all PCRapplications and allows your choice of buf-fer to be determined by your amplificationneeds.

HOT START

TEMPase Hot Start 2 x Master Mix

TEMPASE HOT START 2 X MASTER MIX

PRODUCT NUMBER

Buffer I





Buffer II





Ampliqon page 8

Ampliqon page 9

HOT START

TEMPase Hot Start 2 x Master Mix Blue

WHEN DO YOU USE TEMPASE HOT START MASTER MIX BLUE?The blue loading dye facilitates direct gel loading and eliminates the need for separate

loading dye for electrophoresis and visualisation after PCR. TEMPase Hot Start 2 x MasterMix Blue is an alternative to TEMPase Hot Start 2 x Master Mix with extra

time saving advantages.

FEATURES

Inactive at room temperature

Premixed 2 x solution

DESCRIPTIONAmpliqon TEMPase Hot Start DNAPolymerase, dNTPs, a blue loading dyeand magnesium chloride are present inTEMPase Hot Start 2 x Master Mix Blue with TEMPase Buffer I (balancedKCl/(NH4)2SO4 buffer system) or TEMPaseBuffer II ((NH4)2SO4 buffer system).

The two TEMPase buffers cover all PCRapplications and allow your choice of bufferto be determined by your amplificationneeds.

Comparison with and without 15-minute hot start activation. TEMPase Hot Start DNA Polymerase (lanes 1 and 2).TEMPase Hot Start 2 x Master Mix Blue (lanes 3 and 4). A DNA marker (lane 5).

TEMPASE COMPARISON

Lane 1 2 3 4 5

TEMPASE HOT START 2 X MASTER MIX BLUE

PRODUCT NUMBER

Buffer I





Buffer II





page 9 Ampliqon

Ampliqon page 10

WHEN DO YOU USE REALQ PCR MASTER MIX?Quantitative polymerase chain reaction (qPCR) is the preferred method for gene analysis. RealQ PCR 2 x Master Mix is well suited for gene expression, genotyping, SNP analysis,pathogen detection, drug target validation and for measuring RNA interference.

FEATURES

High sensitivity

High reproducibility

Wide dynamic range

Premixed 2 x solution

OPTIMUM PERFORMANCE ON STANDARD REAL TIME INSTRUMENTS:

ABI PRISM™

Roche LightCycler™

Mx3000™

Comparison between RealQ PCR 2 x Master Mix and competitor A. Amplification of a 130 bp GAPDH gene fragmentfrom 5 ng of human genomics DNA conducted according to supplier’s instructions.

A variety of additional real time kits are available.Please contact Ampliqon for more information.

DESCRIPTIONRealQ PCR 2 x Master Mix has undergoneextensive validation to ensure high perfor-mance.

Our RealQ kits contain TEMPase Hot StartDNA Polymerase, green dye and ROX refe-rence dye.

RealQ PCR Master Mix for probe is alsoavailable.

REAL TIME

RealQ PCR 2 x Master Mix

Fluorescence (dRn)11.4

10.4

9.4

8.4

7.4

6.4

5.4

4.4

3.4

2.4

1.4

0.4

-0.61 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 Cycles

RealQ PCR Master Mix RealQ PCR Master Mix

Competitor A Competitor A

AMPLIFICATION PLOT

REALQ PCR 2 X MASTER MIX

PRODUCT NUMBER

For probe



With green dye



Ampliqon

HIGH FIDELITY

AccuPOL DNA Polymerase

WHEN DO YOU USE ACCUPOL DNA POLYMERASE?AccuPOL DNA Polymerase has proofreading abilities and is targeted at PCR applications,

which require high fidelity or blunt endings.

FEATURES

AccuPOL DNA Polymerase is a thermo-stable enzyme

Processes <3 kb

DESCRIPTIONAccuPOL DNA Polymerase exhibits both 5’ 3’ DNA polymerase activity and 3’ 5’proofreading exonuclease activity.AccuPOL kits include an extra 25 mMMgCl2 concentration if higher MgCl2 is required for specific reactions.

AccuPol DNA Polymerase comes in analternative Tween free version.

* The error rate equals mutation frequency per base per duplication.

FIDELITY COMPARISONS OF THERMOSTABLE DNA POLYMERASES USING THE LACIOZ ASSAY

ENZYME ACCUPOL PFU TAQ

Error rate* (fidelity) 1.1 x 10-6 1.3 x 10-6 18.0 x 10-6

Elongation rate (bases/sec) 30 25 61

ACCUPOL DNA POLYMERASE

PRODUCT NUMBER

Standard high fidelity

210302 250 units

210303 500 units

210304 1000 units

210306 2500 units

Tween free

210402 250 units

210403 500 units

210404 1000 units

210406 2500 units

WHEN DO YOU USE TAQ DNA POLYMERASE?For routine PCR applications, which require high yield and reliable DNA amplification.

FEATURES

A thermostable recombinant DNApolymerase

Enzyme isolated from Thermus Aquaticus

Molecular weight of approximately 95 kDa

Leaves an A’ overhang

DESCRIPTIONAmpliqon Taq DNA Polymerase exhibitsboth a 5’ 3’ DNA polymerase and a 5’ 3’ exonuclease activity. Taq DNAPolymerase has no proofreading ability.

TAQ

Taq DNA Polymerase

AMPLIQON TAQ DNA POLYMERASE

PRODUCT NUMBER

Glycerol free

100103 500 units

100104 1000 units

Standard Taq

110102 250 units

110103 500 units

110104 1000 units

110106 2500 units

110107 5000 units

110108 10000 units

PRODUCT NUMBER

Mg free and Standard Buffer

110203 500 units

110204 1000 units

110208 10000 units

Tween and Triton free

110303 500 units

110304 1000 units

110308 10000 units

Combination Buffer

110403 500 units

110404 1000 units

110406 2500 units

110408 10000 units

PRODUCT NUMBER

Mg free and Ammonium Buffer

110503 500 units

110504 1000 units

110506 2500 units

110508 10000 units

20 mM MgCl2 and Ammonium Buffer

111304 1000 units

+ dNTP

115102 250 units

115103 500 units

115104 1000 units

115108 10000 units

Ampliqon page 12

Ampliqon

ENZYME DILUTION Comparison between Ampliqon Taq DNA Polymerase and four competitors.Amplification of a 160 bp fragment of the human phenylalanine hydroxylase (PAH) gene. Each amplification has been conducted according to supplier’s instructions.

Enzyme dilution

COMPARISON BETWEEN AMPLIQON TAQ DNA POLYMERASE AND COMPETITORS

Enzyme dilution

Ampliqon

1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x

Competitor I

Ampliqon Competitor F

Ampliqon Competitor B

Ampliqon Competitor A

1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x

1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x

1 x 2 x 4 x 6 x 1 x 2 x 4 x 6 x

Ampliqon page 14Ampliqon page 14

Ampliqon

WHEN DO YOU USE TAQ REDPOL DNA POLYMERASE?Taq RedPOL DNA Polymerase is an alternative to standard Taq DNA Polymerase.

The red dye provides convenient identification of enzyme tube and confirms complete mixing.

FEATURES

With Ammonium Buffer

DESCRIPTIONIn Ampliqon Taq RedPOL DNA Polymerasethe inert dye has no effect on downstreamprocesses. Taq RedPOL DNA Polymeraseis added directly to the reaction mix.

TAQ

Taq RedPOL DNA Polymerase

TAQ REDPOL DNA POLYMERASE

PRODUCT NUMBER

200303 500 units

200304 1000 units

200306 2500 units

200307 5000 units

200308 10000 units

200309 20000 units

Ampliqon page 16Ampliqon page 16Ampliqon page 16

WHEN DO YOU USE TAQ DNA POLYMERASE MASTER MIX?Taq DNA Polymerase Master Mix is an alternative to Taq DNA Polymerase. Taq DNAPolymerase Master Mix offers reduced set-up time and eliminates the risk of contaminationof reaction components. Fewer handling steps lead to better reproducibility, which makes Taq DNA Polymerase Master Mix suitable for standard tests.

FEATURES

Premixed solution

Minimal optimisation

Fewer pipetting steps

DESCRIPTIONTaq DNA Polymerase Master Mix is a readyto use 1.1 x or 2.0 x reaction mix. TaqDNA Polymerase Master Mix is composedof Ampliqon Taq DNA Polymerase, theNH4+ buffer system, dNTPs and magne-sium chloride.

TAQ

Taq DNA Polymerase Master Mix

TAQ DNA POLYMERASE MASTER MIX

PRODUCT NUMBER

1.1 x and 1.5 MgCl2




1.1 x and 2.0 mM MgCl2












Ampliqonpage 17 Ampliqon

Ampliqon page 18

WHEN DO YOU USE TAQ DNA POLYMERASE MASTER MIX RED?Taq DNA Polymerase Master Mix Red is an alternative to Taq DNA Polymerase Master Mixwith the same advantages. The inert red dye and stabiliser are extra features, which allowfor direct loading onto your agarose gel for analysis.

FEATURES

No need for separate loading dye

Taq DNA Polymerase Master Mix Red is ready-made for electrophoresis and visualisation

Fewer handling steps

DESCRIPTIONTaq DNA Polymerase Master Mix Red is aready to use 1.1x or 2.0x reaction mix. Taq DNA Polymerase Master Mix Red isavailable in two final MgCl2 concentrations:1.5 mM and 2.0 mM.

Taq Polymerase Master Mix Red is com-posed of Ampliqon Taq DNA Polymerase,the NH4+ buffer system, dNTPs and magnesium chloride.

TAQ

Taq DNA Polymerase Master Mix Red

TAQ DNA POLYMERASE MASTER MIX RED

PRODUCT NUMBER

















Ampliqon page 18Ampliqon page 18

Taq DNA Polymerase Master Mix Red is an Ampliqonbest seller.

Ampliqon

NUCLEOTIDES

DNTP MIX

PRODUCT NUMBER

500005 25 mM / 2 x 1 ml

501004 12.5 mM / 2 x 500 µl

501007 12.5 mM / 8 x 500 µl

502003 10 mM / 1 x 500 µl

502004 10 mM / 2 x 500 µl

502005 10 mM / 5 x 500 µl

502007 10 mM / 8 x 500 µl

503009 2.5 mM / 10 x 1 ml

DNTP SET

PRODUCT NUMBER

511104 100 mM / 4 x 250 µl

511107 100 mM / 16 x 250 µl

511109 100 mM / 80 x 250 µl

511120 100 mM / 4 x 2 ml

DATP, DCTP, DGTP AND DTTP

PRODUCT NUMBER

521102 25 µmol / 1 x 250 µl

521202 25 µmol / 1 x 250 µl

521302 25 µmol / 1 x 250 µl

521402 25 µmol / 1 x 250 µl

DNTP

FEATURES

PCR amplification components

For DNA polymerisation, DNA labelling and sequencing processes

High purity: >98% by HPLC

pH 7.5

dNTP Mix

Ready to use solution

Mix of dATP, dCTP, dGTP and dTTP

dNTP Set

Set of dATP, dCTP, dGTP and dTTP

dATP, dCTP, dGTP and dTTP

Either dATP, dCTP, dGTP or dTTP

Ampliqon page 20Ampliqon page 20Ampliqon page 20

WHAT IS ONETEMP?OneTEMP is a generic term for a custom-made series of DNA/RNA amplification kits fordetection of bacteria, parasites and viruses. A common feature of the OneTEMP series

is that thermocycling is made superfluous. OneTEMP covers ten different isothermal technologies, which are as sensitive and specific as standard PCR reactions and in addition often more tolerant to biological components. Isothermal amplification

is a time and cost saving alternative to most PCR set-ups.

WHY AMPLIFICATION AT ONE TEMPERATURE?The DNA amplification kinetics in isothermalOneTEMP is a function of the reagent com-ponents themselves. This feature makesyour laboratory work independent of equip-ment limitations.

REAL TIME DETECTIONThe OneTEMP technologies can be transformed into traditional real time kits applicable to standard laboratory fluorescence detection and point of caredevices.

ABOUT ONETEMPYou have a PCR reaction that you want to transform into an isothermal version.Provide us with primers and template andwe will find the right isothermal amplifica-tion technology for your need.

ONETEMP IS SUITABLE FOR

Genomics amplification

cDNA/RNA amplification

REQUIRES

From one to four primers

Between 37˚C - 65˚C

From one to several enzymes (enzyme mixes)

Some kits require denaturation steps

HOW?The Ampliqon science team offers expertknowledge in transforming your PCR reaction into an isothermal version suitablefor your specific need and set-up.

FOR MORE INFORMATION: [email protected]

NOTE: your present PCR reaction analoguemust already be working in your laboratory.

ISOTHERMAL AMPLIFICATION

OneTEMP custom-made

Pro

duct

exa

mpl

e

page 21 Ampliqon

Ampliqon page 22

PRICESPlease see our separate price list issuedwith this catalogue.

SAMPLES

In connection with orders

Limited quantity

No dNTP samples

No Genomic Amplifier samples

SAMPLE SIZE

20 reactions

25 units

50 units

HOW TO ORDEREmail: [email protected]: +45 70201179

Order confirmation and shipping notification within 24 hours.

SHIPPING ON DRY ICE

International: air freight

Denmark: road or rail freight

Shipping fee depends on shipping agent, destination and weight

PACKAGING

Minor fee for packaging and dry ice depending on quantity and box size (small, medium and large box sizes)

CONTACTAmpliqon A/SLiterbuen 11DK-2740 [email protected]

Phone: +45 70201169Fax: +45 70201179

VAT No.: 26388899

AMPLIQON BANK INFORMATIONNykredit Bank A/SErhvervs- og investeringscenter Glostrup

Hovedvejen 108DK-2600 GlostrupDenmark

Account: 5470-7025886Swift: NYKBDKKKIban: DK8854700007025886

Practical information

Ampliqon

In addition to our PCR enzyme production,Ampliqon is the supplier of a comprehen-sive bioreagent product line.

We manufacture about 10000 different bioreagents for Danish hospitals, life science institutions and industries. Allcustom-made and on demand.

Ampliqon bioreagents

SOME EXAMPLES:Aga ELISA wash buffer, ammonium acetate in MilliQ water, barbiturate buffer, blood beads, bromine water,DEPC water, buffered formalin, glucose,chloramphenicol, potassium chloride,artificial sweat, artificial urine, sodiumacetate, paracetamol, redistilled water,salicylic acid, sterile buffer glass, swabfluid, sulfuric acid, silver nitrate, urologi-cal fixative, vaseline, cotton swab, yeast extract agar …

AMPLIQON A/S

Literbuen 11

DK-2740 Skovlunde

Denmark

www.ampliqon.com

[email protected]

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