amplifcation-refractory mutation system (arms-pcr) in scd molecular diagnosis introduction to...
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AMPLIFCATION-REFRACTORY MUTATION SYSTEM(ARMS-PCR) IN SCD
MOLECULAR DIAGNOSIS
INTRODUCTION TO ARMS-PCR
EXON 1 EXON 2 EXON 3
6A.
EXON 1 EXON 2 EXON 3
6B.
gAgcTc
gTgcAc
A
T
amplicon
amplicon
common
common
ARMS-PCR IN SCD DIAGNOSIS
EXON 1 EXON 2 EXON 3
6
EXON 1 EXON 2 EXON 3
6
A.
gAgcTc
gTgcAc
T
A
B.
common
common
ARMS-PCR cont.
GEL ELECTROPHORESIS
GEL ELECTROPHORESIS cont.
6
EXON 1 EXON 2gAgcTc
control amplicon
A allele amplicon
A
Outer 2
A
Outer 1 “usual”
“altered”
A ALLELE TETRA-ARMS
6
EXON 1 EXON 2gTgcAc
A
control amplicon
Outer 2
A
Outer 1
S allele amplicon
“usual”
“altered”
A ALLELE TETRA-ARMS
TETRA-ARMS PCR ELCTROPHORESIS