trichodesmium cultures grown under different co 2 and temperatures

Trichodesmium cultures grown under different CO2 and temperatures

Hutchins et al. (2007)

Meta analysis (Findlay et al. 2011) on E. huxleyi cultures shows that after pCO2 and TA, P concentration controls PIC:POC response

Study PIC:POC Growth rate DaylengthIglesias-Rodriguez et al. (2008) ↑ ↓ 12hUnpub. data, Bretherton ↔ ↓ 14hHoppe et al. (2011) ↓ ↔ 18hShi et al. (2009) ↓ ↔ 24h

0 200 400 600 800 1000 1200 14000

0.2

0.4

0.6

0.8

1

1.2

1.4

Long daylight periodLinear (Long daylight period)

pCO2 (µatm)

PIC:

POC

14h 24h0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

AmbientHigh CO2

L:D Cycle

µ (d

-1)

14h 24h

-0.4

-0.2

0

0.2

0.4

0.6

0.8

AmbientHigh CO2

L:D Cycle

Cocc

osph

ere

thic

knes

s (µm

)

14h 24h0

0.1

0.2

0.3

0.4

0.5

0.6

Ambient High CO2

L:D Cycle

Fv/F

m

Fv/Fm decreases slightly in

continuous light cultures – stress?

14h daylight period: calcification maintained, but at the cost of growth

rate

24h daylight period:no change in growth rate,

but cultures produce excess coccolithes

Species Strain Date of isolation

Location of isolation

Emiliania huxleyi PML 70-3 02/07/2011 Northern North Sea

Emiliania huxleyi PML 124-3 25/06/2011 Bay of Biscay

Emiliania huxleyi RCC962 29/10/2004 French Polynesia

Gephyrocapsa oceanica RCC1804 09/12/2008 Sipadan, Malaysia

Coccolithus pelagicus PLY 182 1958 English Channel

Ochrosphera sp. RCC1366 22/02/1998 Palau, Micronesia

E. huxleyi G. oceanica0

0.1

0.2

0.3

0.4

0.5

0.614:10h

AmbientHigh CO2

Species

µ (d

-1)

E. huxleyi G. oceanica0

0.1

0.2

0.3

0.4

0.5

0.6

0.724:0h

AmbientHigh CO2

Species

µ (d

-1)

E. huxleyi G. oceanica0

0.1

0.2

0.3

0.4

0.514:10h

AmbientHigh CO2

Species

Cocc

osph

ere

thic

knes

s (µm

)

E. huxleyi G. oceanica

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.424:0h

AmbientHigh CO2

Species

Cocc

osph

ere

thic

knes

s (µm

)

E. huxleyi G. oceanica0

0.1

0.2

0.3

0.4

0.5

0.6

14:10h

AmbientHigh CO2

Species

Fv/F

m

E. huxleyi G. oceanica0

0.1

0.2

0.3

0.4

0.5

0.6

24:0h

Ambient High CO2

Species

Fv/F

m

Species/Strain 14h 24h

E. huxleyi NZEH Calcification ↔µ ↓

µ ↔, produces lose lithes

E. huxleyi 962Thin coccosphere calcification ↔µ ↓

µ ↔, produces lose lithes

G. oceanica Calcification ↓µ ↓

µ ↓, produces lose lithes

Shipboard experiments from the Arctic Circle and Southern Ocean

Meta-analysis of all OA literature to see whether or not L:D controls the observed response

L:D cycle is an important consideration for OA experiments

Discrepencies in OA response can be explained by L:D cycle

Local changes in L:D cyclesin nature as climate changes

Acknowledgements

Supervisors Dave Suggett and Tracy Lawson

Richard Geider the Algal Research Group at University of Essex

Mark Moore and Alex Poulton

I am in the third year of my PhD and looking for more research opportunities!lmjbre@essex.ac.uk