tre launch training version canada- april 2009

GNLDInternational

GNLD Business Plan

JOHN R. MILLER

Vice President,

Science & Technology

SAB Member

Ancient Wisdom

Modern Science

Timeless Benefits

NEW FRONTIERS IN HEALTH AND LONGEVITY

FLAVONOID COMPLEX

Research abstract as published in the FASEB (Federation of American

Societies for Experimental Biology) Journal - 1998

ANTIPROLIFERATIVE EFFECT OF A FLAVONOID RICH FRUIT AND

VEGETABLE BLEND ON HUMAN BREAST CANCER CELLS. A. Carughi.

Background: The prevention of cancer by administration of food phytochemicals is

currently regarded as a promising avenue for cancer control. Epidemiological studies

have demonstrated the relationship between carcinogenesis and diet. Studies on animal

models have also demonstrated that experimentally induced cancer is preventable with

certain natural compounds in the diet. Many flavonoids have been shown to have potent

anticarcinogenic and antitumor activity. For example, quercetin inhibits carcinogenesis

in a number of models and is able to selectively inhibit a variety of tumor cell growth.

Polymethoxylated citrus flavonoids have been shown to have anti-invasive and anti-

metastatic activities. Purpose: To study the antiproliferative effect of a flavonoid rich

fruit and vegetable blend (Flavonoid Complex Active Blend) on human mammary cancer

cells (MCF7). Method: The polyphenol content of the blend was mg/g (measures

as gallic acid equivalents) and contained mg/g quercetin, mg/g kaempherol,

mg/g epicatechin mg/gm hesperidin, mg/g naringenin and mg/g myricetin.

MCF7 (mammary adenocarcinoma cells) were harvested in RPMI 1640 media and

seeded in 96 well plates. A polar and a non polar extract was prepared using 1:1

dichloromethane-methanol and 70% methanol respectively. The extracts were dissolved

in DMSO, combined and serially diluted in the media. The cells were treated and

incubated and for 3 days. Viable cells were quantified using the neutral Red Dye Uptake.

Doxorubicin was used as the positive control. Results: There was a linear response on

percent inhibition (Figure 1). The concentrations used for regression were 150 to 200

ug/ml (r2 = 0.90). Percent inhibition at these points were 41.5 and 82.1 respectively.

Conclusion: This study demonstrates that a flavonoid rich blend of fruit and vegetable

extracts strongly inhibits growth of human breast cancer cells.

THE WORLD LEADER

Oxidation

NEW FRONTIERS IN HEALTH AND LONGEVITY

Evil Twins of Aging & Disease

Inflammation Oxidation

NEW FRONTIERS IN HEALTH AND LONGEVITY

“SUPER FRUIT” POLYPHENOLS

Risk

Polyphenol

Low Polyphenol Diet High Polyphenol Diet

Polyphenol

Risk

WHAT SCIENCE TELLS US…

Evil Twins of Aging & Disease

Inflammation Oxidation

NEW FRONTIERS IN HEALTH AND LONGEVITY

Nutrition for the Brain

Protection for the Mind

Sharpness for Today

Clarity for Tomorrow

NEW FRONTIERS IN HEALTH AND LONGEVITY

COGNITIVE LONGEVITY

THE NEW FRONTIER

Mental Clarity & Cognitive Longevity

Heart and Circulatory Health

Anti-Inflammatory Anti-Oxidant

Anti-Aging

Cellular Health & Vitality

Joint Flexibility and Function

NEW FRONTIERS IN HEALTH AND LONGEVITY

Ancient Wisdom

Modern Science

Timeless Benefits

BASED IN NATURE,BACKED BY SCIENCE!

NEW FRONTIERS IN HEALTH AND LONGEVITY

POMEGRANATE

Ancient Wisdom Fruit of the Gods

Birth & Eternal Life

Modern Science Cardiovascular

Bone & Joint

Prostate / Breast

Cognitive

AÇAI

Ancient Wisdom Tree of Life

Strength & Energy

Healing

Modern Science Antioxidant

Cell protector

GREEN TEA

Ancient Wisdom Medicine of the Orient

Energy & Alertness

Clarity & Wisdom

Modern Science Cognitive Longevity

Cell Protection

Blueberry Black Currant

Elderberry Red Grape

Bilberry White Grape

Cranberry

“INFLOX” BERRY BLEND

Modern Science

Antioxidant

Anti-inflammatory

Anti-Aging

Cognitive protectors

HIGH-POTENCY ANTIOXIDANTS

RESVERATROL

ALPHA-LIPOIC ACID (ALA)

AGAVE

Naturally sweetened with the nectar of the Agave cactus

THE POWER OF DIVERSITY

Powerful polyphenol protection

Nature’s most potent sources

Individual effects

Synergistic benefits

THE BENEFITS OF DENSITY

Standardized potencies

Assured benefit

Every time

THE VALUE OF TRÉ

•DiversityTotal phenolicsTotal polyphenols

•DensityStandardized amounts of:

PunicalaginsResveratrolAlpha-lipoic acid

THE TRÉ ADVANTAGE

M

110.3 mg/oz82.3 mg/oz

YesYesYes

44.4 mg/oz

?

No

No

No

WORLD CLASS PACKAGING

Ancient Wisdom

Modern Science

Timeless Benefits