the power of small2014.igem.org/files/presentation/virtus-parva_mexico... · 2014-11-25 · some...

The Power of Small

The Bio-NEMS drill

*Patent pending

Resonator

*Patent pending

Image obtained from MEMS & Nanotechnology Exchange.

<https://www.mems-exchange.org/MEMS/what-is.html>

DNA Coiling

*Patent pending

Biological Resonator?*Patent pending

IDEA: Mimic the chromatin’s nucleosome

*Patent pending

Survey

What is a

Microelectromechanical

System (MEMS)?

NEMS

28.77

71.23

Know what a MEMS is

Do NOT know what a MEMs is

*Patent pending Fedder, G.K. ; Carnegie Mellon Univ., Pittsburgh ; Howe, R.T. ; Tsu-Jae King Liu ; Quevy, E.P.

Movie Projection

Disseminate

Nanotechnology and

its contribution to

Synthetic Biology

*Patent pending

Some assumptions were made:

• All NPs are monocrystalline and equal

(Fe3O4, r=11nm and Fe3O4@SiO2, r= 21nm)

• The DNA coiled around acts as a rigid body.

• The force to unbind DNA from the protein is

about 6.3 pN.

6.3 pN

*Patent pending

Some assumptions were made:

• All NPs are monocrystalline and equal

(Fe3O4, r=11nm and Fe3O4@SiO2, r= 21nm)

• The DNA coiled around acts as a rigid body.

• The force to unbind DNA from the protein is

about 6.3 pN.

• DNA mass is insignificant compared to the

NP’s.

• All drags are the same

• Homogeneous medium

• The system can be reduced to a single mass

and drag

6.3 pN

*Patent pending

Bio – NEMS Drill

Lorentz force equation

Drag force equation

The amount of Bohr

magnetons per unit cell

Number of paired

electrons

Charge

Total magnetic force

Total force

Amount of

Nanoparticles

*Patent pending

*Patent pending

The Bio-NEMS drill

• Individual Components:

• Magnetic Nanoparticles

• Peptide

• Cross-linking agent

• DNA strand

*Patent pending

NanoparticleSynthesis• Co-precipitation

Method

• More tan 50 trials

• Functionalization• Biocompatible

*Patent pending

Magnetite

• Superparamagnetic

• 22 nm monodisperse

*Patent pending

PeptideAssembly• Trial 1: SASP

• Trial 2: HU

• DNA – binding

• Electrophoresis

*Patent pending

The Bio-NEMS drill

*Patent pending

Law Initiative

Difficulties with the

importation of reactants

and biological agents!

A law initiative came to be

as a collaboration with the

Tec Monterrey team.

*Patent pending

Is this real?

*Patent pending

Characterization

UV Spectroscopy

260 nm

Blue: Np + DNA

Green: Nanoparticles

Use of plasmids for characterization

of the device

*Patent pending

Bio-NEMS Drill as

a cloning vector

Improve genetic

transformation

*Patent pending

*Patent pending

*Patent pending

And the Brainstorm came…

*Patent pending

*Patent pending

The Test

Does it Work?

*Patent pending

ResultsBBa_K737051 Used as control

and DNA in our system for a traditional transformation and a Electroporation Method

60 to 80 Colonies in our Control against 80 to 100 in the enhanced electroporation

*Patent pending

The Joy of Sharing

*Patent pending

Achievements

Research fund

for students’

projects!!!

Deeper

understanding

of Synthetic

Biology!!!

*Patent pending

Low Budget

project with

great results!!!

*Patent pending

What’s next?

*Patent pending

*Patent pending

*Patent pending

IT IS THE REAL

DRILL!!*Patent pending

iGEM Berlin

*Patent pending

*Patent pending

Marvellous doesn’t always come from complexity