synthetic biology part 1: introduction input output gene a gene b gene c 1
Post on 01-Apr-2015
236 Views
Preview:
TRANSCRIPT
1
Synthetic Biology Part 1:
Introduction
Input
Output
AND
Gene A
Gene B
Gene C
micro.magnet.fsu.edu
Prokaryotic cell
3
Cell-Cell communication in prokaryotes
http://parts.mit.edu/igem07/index.php/Chiba/Communication
4
DNA
RNA
PROTEIN
Transcription byRNA polymerase
Translation byribosomes
Flow of genetic information
5
DNA structure
6
Gene structure
Gene Gene
Promoter Coding sequence Terminator
chromosome
7
RNA
Transcription start site
Promoter
Gene structure
Terminator
8
Promoter structure
9bioap.wikispaces.com
Transcription
10
Transcription
11
Transcription
12
AUG UGA
Ribosome binding siteto initiate translation
Start codon(first amino acid of the protein)
Stop codon(signal to end
protein synthesis)
= untranslated region
5’ 3’
mRNA structure
13
+1
-10Box
TATAA
-35Box
TTGTCA RNA
Core promoter = Binding site for RNA polymerase
In this configuration transcription is ON
RNA Pol
Transcription
14
RNA
+1
RNA PolA
A = Activator of transcription
bad promoter
Transcription
15
+1
-10box
-35box
operator
R
R = Repressor
In this configuration RNA Polymerase cannot bindtranscription is OFF
X
Repression of transcription
16
• The lactose operon of E. coli
R
lacIrepressor R
-35 O -10
Transcription is OFF
Active repressor
X
Repression of transcription
17
• The lactose operon of E. coli
R
lacIrepressor
-35 O -10
Transcription is ON
Inactiverepressor = inducer (lactose)
RNA Pol
X
Induction of gene expression
18
AUG UGA5’ 3’
3’ 5’
siRNA
Binding of siRNA causes mRNA degradation
TranslationX
Silencing by antisense RNA
19
AUG UGA5’ 3’
Ribozymes+ aptamer
RNA cleavage
Repression by mRNA cleavage
20
AUG UGA
Ribosome binding siteto initiate translation
Start codon(first amino acid of the protein)
Stop codon(signal to end
protein synthesis)
= untranslated region
5’ 3’
mRNA structure
21
Translation
22
brooklyn.cuny.edu
The genetic code
23
Protein structure
biochem.arizona.edu
top related