semester -2 sem.€¦ · dr.veena m. mahajan. dna damage and repaire content ... 1.excision repair...
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J. M. PATEL ART COMMERCE AND
SCIENCE COLLEGE
SEMESTER -2nd sem.
PRESENTED BY
DR.VEENA M. MAHAJAN
DNA DAMAGE AND
REPAIRE
CONTENT
Introduction
Types of DNA Damage
Types of mutation
DNA repair mechanism
Excision Repair
Mismatch Repair
Recombination repair
Double strand DNA break repair
WHAT IS DNA
A nucleic acid that carries the
genetic in formation in cell &some
viruses consisting a two long chain of
nucleotide twisted in double helix
DNA DAMAGES
Types of damages
Mutation
Types of mutation
1. point mutation
transition and transversion
2. frameshift mutation
addition and deletion
1. POINT MUTATION
TRANSVERSION AND TRANSITION
2.FREAMSHIFT MUTATION
Addition and deletion causing shift
1.mutation in single stranded break :-ex. peroxide
2.DOUBLE STRAND BREAK (DS BREAK)
T-T dimmer mutation :-
Repair enzyme
photolygase in this dimmer.
•INTERCALATION
AFFECT OF DYES IN MUTATION
Acridine dyes : yellow, orange , red
Ethidium bromide :it’s a aromatic &
flourscent compound
Acriflovin :It is antiseptic & orange,
brown in colour
Proflavin : used urinary antiseptic
•CROSSLINKING MUTATION
like mitomycin C
•APURINATION & APYRIMIDINATION
BREAKAGE OF N-GLYCOSYLIC BOND IN BASE
DNA REPAIR MECHANISM
1.Excision repair
it consist of a two repair process
DNA glycosylase
NUCLEOTIDE EXCISION REPAIR
2.MISMATCH REPAIR
It consist two types of repair
mechanism
Recombination repair
Double strand DNA break
mechanism
1. RECOMBINATION REPAIR
BREAK DNA DOUBLE STRAND
REPAIR
THANK YOU
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