review of microscopes
Post on 22-Feb-2016
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Review of Microscopes
Plant and animal cell structure
3 types of microscopes
Light Electron Scanning/tunnelling
Types of microscopes
Light microscopes
Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords
Microscope Care
EyepieceBody Tube
Revolving NosepieceArm
Objective Lens
StageStage Clips
Coarse FocusFine Focus
Base
DiaphragmLight
Microscope parts
Place the Slide on the Microscope
Use Stage Clips Click Nosepiece to the
lowest (shortest) setting
Look into the Eyepiece Use the Coarse Focus
Using the microscope
Follow steps to focus using low power Click the nosepiece to the longest objective Do NOT use the Coarse Focusing Knob Use the Fine Focus Knob to bring the slide
What can you find on your slide?
Using high power
1. Set up the microscope to view the prepared slides
2. View at least 4 different slides, make a labelled diagram of what you see
3. Classify your cells as being either plant or animal cells
4. Write a simple experimental write up for this experiment
Task 1
Needs the following headingAim: The purpose of the experiment Materials: A list of what was usedProcedure: A step by step description of the processResults: Your labelled diagrams and any other observationsDiscussion: Analysis of results, include similarities and differences between animal and plant cells from your observations, include any errors of difficulties.Conclusion: Write a concluding paragraph about the results and the aim
Write up
Electron Microscopy (TEM) beams of electrons
are used to produce images
wavelength of electron beam is much shorter than light, resulting in much higher resolution with this type of microscope
TEM
The Scanning Electron Microscope (SEM)
uses electrons reflected from the surface of a specimen to create an image
produces a 3-dimensional image of specimen’s surface features
SEM
Resolution
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