protein engineering

Structure-based Protein Design and Engineering

• What can it do for you?

• How it works?

Health, Environment and Technology

Molecular Modelling Mini Tutorial

Protein-based Technologies are widely used in our daily life

Health Environment Technology

Molecular Modelling Mini Tutorial

Proteins Enzymes and Peptides are made of a sequence of aminoacids

1 MASPFALLMV LVVLSCKSSC SLGCDLPETH SLDNRRTLML LAQMSRISPS SCLMDRHDFG 61 FPQEEFDGNQ FQKAPAISVL HELIQQIFNL FTTKDSSAAW DEDLLDKFCT ELYQQLNDLE121 ACVMQEERVG ETPLMNADSI LAVKKYFRRI TLYLTEKKYS PCAWEVVRAE IMRSLSLSTN181 LQERLRRKE

This is the sequence of a well known protein.

Can you guess what it is, what it does and how it performs it function?

Molecular Modelling Mini Tutorial

Protein function can be understoodby its 3D molecular structure

Protein structures can be determined by:

X-ray NMR Homology Modelling

Molecular Modelling Mini Tutorial

Do you have a sequence and want to …

• Know it’s structure

• Understand it’s function

• Improve it’s native properties

• Perform targeted mutations

• Improve structure specific features

Molecular Modelling Mini Tutorial

Bimolecular Modelling can help you!

Active site Protein-proteinaggregation site

Conserved domain

Mobile domainHydrophobicpatch

We model a 3D structure

from your sequence, and provide

detailed structural and dynamic

insights concerning it’s function

Molecular Modelling Mini Tutorial

What about engineering improvedprotein function and structure?

Focus your effort on the most

promising recombinant proteins

predicted by our computer-aided

protein structure design platform

We can perform large scale

aminoacid mutation screening,

so you can focus your effort on

the most promising

recombinant proteins.

Molecular Modelling Mini Tutorial

Molecular Modelling can support your research & development effort on:

• Vaccines

• Antibodies

• Peptides

• Peptidomimetics

• Enzymes

• Other protein-based technologies

Molecular Modelling Mini Tutorial

Dr. Nuno MicaeloResearcher, Aveiro University

nmicaelo@gmail.com