prof. frank's review powerpoint parasites

LAB REVIEW

PARASITES&

BLOOD CELLS

Band Neutrophil

Red Blood Cells

Lymphocyte

Eosinophil

Platelets

Basophil (in horses it is blue)

Monocyte

Segmented Neutrophil

Tapeworm

Hookworm

Heartworm Microfilaria

Roundworm

Coccidia

Whipworm

Scabies Mite

Demodex

Giardia

FLEA

Otodectes Cynotis Mite

Babesia Canis

Sucking Lice…………..Biting Lice

Ehrlichia

Dermacentor Variabilis….Black Leg Tick

Lone Star Tick………American Dog Tick

Dirofilaria immitum….dipeteloma reconditum

Avian Blood

Warble or Cuterebra (fly larvae)

Anaplasma Marginale

Lung Worm

paragonimus kellicotti

Lung Fluke

Eucoleus boehmi

Urine

Physical Exam:

Smell

Color

Turbidity

Foam

Urine

Chemical Exam:pH

Bilirubin

Protein

Occult Blood

Glucose

Ketones

Specific gravity

Nitrites

WBCs

Heartworm Test

Buffy Coat method (hematocrit test)

Modified Knott’s technique

Filter membrane technique/Di-fil

Blood Smear

SNAP: Antigen testing does not detect the actual parasite but the host’s response to the

disease

Steps For Modified Knotts

Mix 1 ml of blood w/ 9ml of 2% formalin in centrifuge

Centrifuge tube at 1300 to 1500 rpm for 5 minutes

Pour off liquid supernatant, leaving sediment at bottom of tube

Add 2-3 drops of stain to sediment. Using pipette, mix sediment w/stain

Place drop of this mixture on glass slide. Apply coverslip. Examine for microfilariae using 10X objective.

Filter MembraneMix 1 ml of blood w/9 ml of lysing solution in 15 ml test tube

Place filter membrane in filter holder, close tightly, do not pinch seal in threads of holder

Connect syringe to filter holder and gently empty through filter holder

Fill syringe with water and repeat above step

Open holder, use thumb forceps to remove filter membrane

Place filter membrane on a clean microscope slide

Add one drop of stain, cover with coverslip, examine for microfilariae

Stages of a Parasites Life Cycle

• 1. oocyst

• 2. Larvae

• 3. nymph

• 4. Pupa

• 5. Adult