pcr product guide - sigma-aldrich

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sigma.com/pcr ORDER: 800-325-30�0 TECHNICAL SERVICE: 800-325-5832�2

Custom Oligonucleotides

Sigma-Genosysoffersacompletelineofcustomoligonucle-otidesforyourresearch.Sigma-Genosyshasmanufacturingsitesaroundtheglobein10countries–Australia,Canada,France,Germany,India,Israel,Japan,Singapore,UKandUSA.Ourproprietaryplatforms,theAbacus™andtheARK™,provideunparalleledthroughput,capacityandqualityresult-inginaffordable,high-qualityoligos.

Sigma-GenosysisISO9001:2000registeredatourmanufacturingsitesinCanada,Germany,Israel,JapanandtheUSA.TheUKmanufacturingsiteisISO14001:1996registered.

Products and Servicesn StandardDNASynthesisandPurification

n SpecialtyPrimers

n LongOligosforArrays

n Real-timePCRProbes

n ModifiedOligos

n OligosforHigh-ThroughputApplications

n GeneSynthesis

n OligoDesignService

Features and Benefitsn Everyoligoisbar-codedforefficientandaccuratehandling

throughouttheproductionprocess

n Anadditionalviallabelisincludedforyourconvenience

n Everyoligoisdeprotectedanddesalted

n 100%QualityControlusingMALDI-TOFMassSpectrom-etry,ElectrosprayIonizationMassSpectrometry(ESI-MS),CapillaryElectrophoresis(CE)orPolyacrylamideGelElectrophoresis(PAGE)

n QualityAssurancedocumentationincludingmolecularmass,sequenceandyieldisincludedwitheveryorder

n Shippingwithin1–2daysforstandard(non-modified)oligos

n Mostmodifiedoligosshipwithin3-5workingdays

n Onlineordertrackingisavailableonourwebsite

n Technicalsupportstaffcanassistwitholigodesignandhelptotroubleshootdifficultassays

Custom Oligonucleotides from Sigma-Genosys

Purity Levels and Guaranteed Minimum Yields*�Purification� Recommended�Method� Applications� 0.025�µmole� 0.05�µmole� 0.2�µmole� 1.0�µmole� 10�µmole� 15�µmole

Desalting PCR,probing,mobilityshift, 3OD 5OD 12OD 40OD 400OD 600OD≤35bases arbitrary10-merapplications, 90µg 150µg 360µg 1,200µg 12,000µg 18,000µg hybridization

Cartridge Allaboveapplications,plus N/A 1OD 3OD 12OD N/A N/A(RPI) sequencing,DNAfinger- N/A 30µg 90µg 360µg N/A N/A≤50bases printing,cyclesequencing, RT-PCR,in situ hybridization, microsatellitepolymorphisms

HPLC Allaboveapplications, N/A 1OD 2.5OD 13OD 130OD 190OD(RPI) plusmutagenesis,S-oligos, N/A 30µg 75µg 390µg 3,900µg 5,700µg≤50bases large-scales,antisense†, andtriplehelix†

PAGE Allaboveapplications,plus N/A 0.5OD 1OD 5OD N/A N/A≤110bases genesynthesisandprimer N/A 15µg 30µg 150µg N/A N/A extension

* Guarantee is for non-modified oligos ≥20 bases; yields for shorter oligos will be proportionally less. S-oligos and other modified oligos will typically contain 30% and 40%, respectively, less yield than unmodified oligos.

† In combination with gel filtration.

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�3Our Innovation, Your Research — Shaping the Future of Life Science

Real-time PCR Probes

Sigma-Genosysoffersacompletelineofreal-timePCRchemistries.Ourbroadportfoliooffersavarietyofprobeandprimeroptionstosuityourindividualneeds.

Ourtechnicalsupportteamisalsoavailabletoassistyouwithyourprobeandprimerdesign.ThisserviceisofferedfreeofchargetoallSigma-Genosyscustomers.Simplycontactyourlocaltechnicalsupportofficeorcompletetheonlinerequestformformoreinformation.

Products and Servicesn Dual-labeledProbes

n MolecularBeacons

n LightCycler®Probes

n Scorpions®Probes

n LNA™Dual-labeledProbes

n LNAMolecularBeacons

nLNALightCyclerProbes

nProbeandPrimerDesignServices

Features and Benefitsn Fullydeprotectedanddesalted.PurifiedbyPAGEorRP-HPLC

n 100%QualityControlusingMALDI-TOFMassSpectrome-try,PolyacrylamideGelElectrophoresis(PAGE)orRP-HPLC

n Everyprobeisdelivereddriedinambertubestoensuredyeintegrity

n Onlineordertrackingisavailableonourwebsite

n Ourtechnicalserviceteamcanassistwithprobedesign

Shipping ScheduleLightCyclerprobes: 4to5workingdaysDual-labeledfluorogenicprobes: 5to6workingdaysMolecularBeaconsprobes: 5to6workingdaysScorpionsprobes: 7to10workingdays

Custom Dual-Labeled Probe & Molecular Beacon Formats

Guaranteed OD Yield

Approximate No. of nmoles*

Approximate No. of Reactions**

1 5 1,000

3 15 3,000

5 25 5,000

10 50 10,000

50 250 50,000

100 500 100,000

*1OD=5nmoles=30µgfora20-meroligo.Nmoleamountwillvarybasedonoligolength

**Numberofreactionsisbasedona25µlreactionsize,finalprobeconcentrationof200nM.

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sigma.com/pcr ORDER: 800-325-30�0 TECHNICAL SERVICE: 800-325-5832��

Custom OligonucleotidesCustomer/Technical Support

United States/North AmericaTel:1-800-2345-DNAcustsvc@sial.comtechsrv@sial.com

Australia/New Zealand 1800800097(Australia)0800936666(NewZealand)(+61)266228614

Austria: Tel:497329972950E-mail:genosys_de@europe.sial.com

Belgium: Tel:0800-14747E-mail:becustsv@europe.sial.com

Canada(866)677-0277customdna@sial.com

Czech Republic: Tel:+420246003200E-mail:Czetechsv@europe.sial.com

Denmark: Tel:43565910E-mail:denorder@europe.sial.com

Finland: Tel:(09)3509250E-mail:finorder@europe.sial.com

France: Tel:0800779508E-mail:FRASpecifics@europe.sial.com

Germany+49/(0)7329/97-2950genosys_de@europe.sial.com

Greece: Tel:302109948010E-mail:GRCustSV@europe.sial.com

Hungary: Tel:0612359054E-mail:info@sigma.sial.hu

India+91(80)51127272india@sial.com

Ireland: Tel:1800200888E-mail:eicustsv@europe.sial.com

Israel1-800-70-2222isrcutserv@europe.sial.com

Italy: Tel:800536373E-mail:itclient@europe.sial.com

Japan0133-75-7311(+81)133735005genosys@genosys.co.jp

Netherlands: Tel:08002288032E-mail:nlcustsv@europe.sial.com

Norway: Tel:23176060E-mail:nororder@europe.sial.com

Poland: Tel:0618290100E-mail:pltechsv@europe.sial.com

Portugal: Tel:800202180E-mail:pocustsv@europe.sial.com

Spain: Tel:900101376E-mail:escustsv@europe.sial.com

Sweden: Tel:020-350510E-mail:sweorder@europe.sial.com

Switzerland: Tel:41817552650E-mail:cheorders@europe.sial.com

United Kingdom/Europe 0800269016(UKOnly)+441440767000(Europe)customerservices@sigma-genosys.co.ukeurtechserv@europe.sial.com

Internetordersmaybesubmittedbyvisitingourwebsite:sigma.com/oligosOnlineordertrackingisavailableforordersplacedthroughtheInternet.

Fordetailsabouthowtoorderbyfaxore-mail,pleasecontactyourlocalcustomersupportteam.

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