observational record of pre-mating and mating behaviors of different families of butterflies in some...

Observational Record of Pre-mating and Mating Behaviors of

Different Families of Butterflies in Some Selected Areas of Bangladesh

Roll: 901Reg. No.: HA-2571

Department of ZoologyUniversity of Dhaka

INTRODUCTION

• Butterfly

• Pre-mating and mating behaviorRelation with abiotic factorsDifferent vegetation layersDifferent time schedules

MATERIALS AND METHODS

• Study Period: March’15 to February’16

• Experimental procedures:(Rutowski 1997; Lederhouse 1995)

• Emergent Sampling method

• Statistical analysis:- Correlation-coefficient- ANOVA- Regression

Figure. Map of Bangladesh (red circles are study areas)

RESULTS AND DISCUSSION

• Perchingbehavior

• Patrolling behavior

• Hair-pencil behavior

• Pupal matingFigure. Observed pre-mating and mating occurrences

Pieridae

Danaid

ae

Satyr

idae

Nymphali

dae

Hesperiid

ae

Papilio

nidae

Lycae

nidae

0

5

10

15

20

25

107

5

1519

11

21

3 24

1

63

7Pre-mating Mating

Butterfly families

No.

of s

peci

es

Figure. Fluctuation of observed pre-mating occurrences throughout the study period (March’15 to February’16)

March

AprilMay June

July

August

Septem

ber

October

November

December

January

February

0

10

20

30

40

50

60

70

LycaenidaePapilionidaeHesperiidaeNymphalidaeSatyridaeDanaidaePieridae

Months

Abso

lute

freq

uenc

y(p

re-m

ating

)

Figure. Fluctuation of observed mating occurrences throughout the study period (March’15 to February’16)

March

AprilMay June

July

August

Septem

ber

October

November

December

January

February

0

2

4

6

8

10

12

14

LycaenidaePapilionidaeHesperidaeNymphalidaeSatyridaeDanaidaePieridae

Months

Abso

lute

freq

uenc

y(m

ating

)

Figure. Fluctuation of pre-mating and mating occurrences in relation to different abiotic factors

45 50 55 60 65 70 75 800

10203040506070

Pre-mating Mating

Humidity (%)

Abso

lute

freq

uenc

y

0 1 2 3 4 5 6 7 80

10203040506070

Pre-mating Mating

Precipitation (mm)

Abso

lute

freq

uenc

y

10 10.5 11 11.5 12 12.5 13 13.50

10203040506070

Pre-mating Mating

Day Length (hour)

Abso

lute

freq

uenc

y

18 20 22 24 26 28 300

10203040506070

Pre-mating Mating

Temperature (°C)

Abso

lute

freq

uenc

y

• Statistical analyses (In case of observed pre-mating occurrences)

• Correlation coefficient values are,

Butterfly Families

Humidity Precipitation Day length Temperature

Pieridae -0.44 -0.25 -0.07 0.05

Danaidae 0.05 0.37 0.40 0.43

Satyridae 0.10 -0.40 -0.07 0.29

Nymphalidae -0.80 -0.54 -0.29 -0.14

Hesperidae -0.37 -0.65 -0.55 -0.23

Papilionidae -0.12 -0.14 -0.02 0.47

Lycaenidae -0.44 -0.40 -0.11 0.10

F= 227.23 Fcrit= 1.928 P= 3.97E-63

• Statistical analyses (In case of observed mating occurrences)

• Correlation-coefficient values are

Butterfly

Families

Humidity Precipitation Day length Temperature

Pieridae -0.66 -0.77 -0.80 -0.33

Danaidae 0.76 0.79 0.94 0.98

Satyridae 0.43 0.22 0.20 0.73

Nymphalidae

Hesperidae -0.21 -0.61 -0.42 0

Papilionidae

Lycaenidae 0.14 -0.24 -0.25 0.05

F= 241.31 Fcrit= 1.99 P= 1.49E-44

Figure. Observed pre-mating and mating of butterflies in different vegetation layers

Pieridae Danaidae Satyridae Nymphalidae Hesperiidae Papilionidae Lycaenidae0

20

40

60

80

100

120

Herb Shrub Tree

Different butterfly families

Pre-

mati

ng fr

eque

ncy

Pieridae Danaidae Satyridae Nymphalidae Hesperidae Papilionidae Lycaenidae02468

101214

Herb Shrub Tree

Different butterfly families

Mati

ng fr

eque

ncy

P= 0.0003

Figure. Observed pre-mating and mating occurrences of butterflies in different time schedules

9.00-10.00 10.00-11.00 11.00-12.00 12.00-13.00 13.00-14.00 14.00-15.00 15.00-16.000

20

40

60

80

100

120

140

30

59

95

117

61

46

21

211

16 19

7 103

Pre-mating Mating

Time schedule

Abso

lute

freq

uenc

y

CONCLUSION

- Monthly fluctuation observed- Abiotic factors had significant impact- Environmental biassness- True interest in specific vegetation layer- Peak interest towards specific time schedules of a day

ACKNOWLEDGEMENT• Prof. Dr. M. A. Bashar• Prof. Dr. Md. Anwarul Islam• All teachers and stuffs• EBBL researchers• NST

Thank you.