molecular weight markers
Post on 05-Feb-2016
276 Views
Preview:
DESCRIPTION
TRANSCRIPT
・DNA Step Ladders
・OneSTEP Ladders
・Ladders for 10minute separation
・Wide Range Ladders
・Markers & OneSTEP Markers
・DGGE Markers
・PCR Marker Makers
・Easy SeparatorTM
・Negative Gel Stain MS Kit
・SuperSepTM Ace
・SuperSepTM DNA
・RNA Size Standard Markers
・RNA Step Ladder
・Loading Buffers
RNA Si St d
・Prestain markers
・ Western marker
・ Biotinylated marker
・ Markers for CBB dyeing
co.jp
・PCR Marker Makers
・Easy SeparatorTM
・Loading Buffers
Molecular-WeightMarkers
DNA Ladder
10 bp DNA StepLadder (10-100 bp)
25 bp DNA StepLadder (25-300 bp)
25 bp DNA StepLadder (25-500 bp)
50 bp DNA StepLadder (50-800 bp)
DNA Step Ladder(10-300 bp)
100 bp DNA Step Ladder(0.1-1.5 kbp)
OneSTEP Ladder 50(0.05-2 kbp)
OneSTEP Ladder 100(0.1-2 kbp)
DNA Step Ladder (1~6)for low-molecular-range analysis.
Loading:1µL/lane
Loading:5-10µL/lane
Loading:5µL/lane
Concentration:0.69µg/5µL
Concentration:0.755µg/5µL
Loading:5µL/lane
1% Agarose
5% Agarose 21
3% Agarose 21
2% Agarose
3% Agarose 21
2% Agarose
3.5% Agarose
2% Agarose
1% Agarose S
1% Agarose S
1% Agarose S
2% Agarose S
1
5 6 7 8
2 3 4
9 10 11 12
OneSTEP Ladder 500 (0.5-5 kbp)
OneSTEP Ladder 1 kb(1-10 kbp)
OneSTEP LadderSupercoiled Plasmid
Gene Ladder 100(0.1-2 kbp)
Loading:5µL/lane
Loading:5µL/lane
100
(bp)
(bp) (bp) (bp)(ng/5µL)
908070605040302010
(bp)(ng)500 60
(bp)
(bp) (kbp) (kbp)
800400 60 300 60 250 60 200 120 175 80 150 80 125 80 100 120 75 80 50 80 25 80
700600500400300
150200
10050
1,000900
1,500
800
717600500400
286
200
100
2,0001,8001,6001,4001,2001,000900800700600500400300200100
10090807060100454035305020151010
30020015010075503525201510
5,0004,500
3,5003,0002,5002,000
1,500
1,000
4,000
500
10.09.08.07.06.05.04.03.0
2.0
1.0 100
500
200
(bp)(ng/5µL)
300400
6007008009001,000
1,5002,000
100
75
203040
3035404550
75100
10.09.08.0
6.05.0
4.0
3.0
7.0
OneSTEP Ladder (7~12)for simultaneous measurement of DNA size and amount.
each fragment: 50ng/5µL
each fragment: 50ng/5µL
each fragment: 30ng/5µL
Concentration: 0.64µg/5µL
DN
A La
dder
DN
A La
dder
Readyto
use
With dye and glycerol already added, it is ready to be applied to the agarose gel.
6xBlue/OrangeLoading
Dye
6xBlue/OrangeLoading
Dye
-20℃ -20℃ -20℃ -20℃
Readyto
use
Readyto
use
Readyto
use
Readyto
use
Readyto
use
Readyto
use
-20℃
-20℃
Readyto
use
-20℃
300275250225200175150125100755025
(bp)
(bp)(ng/5µL)2,0001,5001,000900800700600500400450350300250200150100
50
1007550454035307522.52017.51537.5107.510
100
6xLoading
Dye
6xLoading
Dye
6xLoading
Dye
2
1% Agarose S
1% Agarose S
2% Agarose
4% Agarose 21
2% Agarose
2% Agarose
13 14 15 16
17
18
DNA Step Ladder(10-200 bp)
DNA Step Ladder(100-5,000 bp)
DNA Step Ladder(50-1,500 bp)
DNA Step Ladder(500-10,000bp)
Gene Ladder Fast 1(4 bands:0.1-2 kbp)
Gene Ladder Fast 2(6 bands:0.1-10 kbp)
(bp) (bp) (bp) (bp)(ng/5µL)
(bp)(ng/5µL)
200100
50
20
2,000 251,000 25500 25100 25
50
200
400
850
1,500
10
100
400
850
2,000
(bp)
5,00010,0004,0002,000
1,000
500
10,000 254,000
252,000
25
1,00025
500 25100 25
Loading:20µL/lane
Loading:20µL/lane
Loading:20µL/lane
Loading:20µL/lane
Ladder for 10minute separation (13~18)
123456
7
8
9
10
1112131415161718
10 bp DNA Step Ladder (10-100 bp) 25 bp DNA Step Ladder (25-300 bp) 25 bp DNA Step Ladder (25-500 bp) 50 bp DNA Step Ladder (50-800 bp) DNA Step Ladder (10-300 bp) 100 bp DNA Step Ladder (0.1-1.5 kbp)
OneSTEP Ladder 50 (0.05-2.0 kbp)
OneSTEP Ladder 100 (0.1-2 kbp)
OneSTEP Ladder 500 (0.5-5 kbp)
OneSTEP Ladder 1 kb (1-10 kbp)
OneSTEP Ladder Supercoiled PlasmidGene Ladder 100 (0.1-2 kbp)DNA Step Ladder (10-200 bp), Fast Separation TypeDNA Step Ladder (50-1,500 bp), Fast Separation TypeDNA Step Ladder (100-5,000 bp), Fast Separation TypeDNA Step Ladder (500-10,000 bp), Fast Separation TypeGene Ladder Fast 1(4 bands:0.1-2 kbp)Gene Ladder Fast 2 (6 bands:0.1-10 kbp)
040-28721292-69001044-30711544-02311298-66301296-68801319-06044315-06041311-06043317-05244313-05241319-05243317-05364313-05361319-05363314-05374310-05371316-05373317-05381316-06951292-66201290-61001296-61101292-61201317-06981314-06991
32.5 μg (50 μL) 100 μg 500 μL 85 μg (250 μL) 50 μg 30 μg 25 μL500 μL500 μL ×2 25 μL500 μL500 μL ×2 25 μL500 μL500 μL × 3 25 μL500 μL500 μL × 3500 μL500 μL10 μg (0.5mL) × 210 μg (0.5mL) × 210 μg (0.5mL) × 210 μg (0.5mL) × 2500 μL × 2500 μL × 2
Code No.Number Product name Package Size Note
: Nippon Gene’s products
Store at ー20℃Store at 2~10℃
:
NO DISPLAY:
-20℃
Concentration: 0.1µg/5µL
Concentration: 0.15µg/5µL
Readyto
use
-20℃
Readyto
use
-20℃
Readyto
use
-20℃
Readyto
use
Readyto
use
Readyto
use
-20℃
6xLoading
Dye
6xLoading
Dye
6xLoading
Dye
6xLoading
Dye
3
DNA Step Ladder Mix(0.08-10 kbp)
Smart Ladder(0.2-10 kbp)
Gene Ladder Wide 1(0.1-20 kbp)
Gene Ladder Wide 2(0.1-20 kbp)
Marker 1
(λ/Hind Ⅲ)
Marker 2
(λ/Hind Ⅲ・EcoRⅠ)
Marker 3
(λ/Hind Ⅲ+λ/EcoRⅠ)
Marker 4
(φX174/Hae Ⅲ)
3% Agarose 21
1% Agarose S
1% Agarose S
1% Agarose S
1% Agarose S
1% Agarose
1% Agarose
3% Agarose 21
1% Agaose S
0.3% Agarose H
0.3% Agarose H
Marker 5
(φX174/HincⅡ)
Marker 6
(λ/StyⅠ)
Marker 7GT
(T4 GT7+T4 GT7/BglⅠ)
Marker 8GT
(T4 GT7+T4 GT7/BglⅡ)
20,00010,0007,000
600500400200100
5,0004,0003,000
2,0001,5001,000900800700
100 50 35
30 75 40 20100
75 40 30
100 30 504540 35
(ng/20µL)(bp)
(kbp) (kbp) (kbp) (bp)
(ng/5µL)(bp) (ng/5µL)(bp) (ng/5µL)(bp)
(bp) (kbp) (kbp) (kbp)
10,0008,0006,0005,0004,000
10,0008,0006,0005,0004,0003,0002,5002,0001,5001,000800600400200
100806050403025201510080604020
20016012010080
3,000 602,500 502,000 40
1,500 32
1,030 200900 180800 160700 140600 120500 200400 80300 60200 40100 2080 16
20,00010,0007,0005,0004,0003,000
2,0001,500
1,0001,300
700500400300200100
1005035754030
10030
10026
3575403020100
1% Agarose
19 20 21 22
27 28 29 30
23 24 25 26
23.13 21.23
5.15
4.98
4.27
3.52
2.02
1.91
1.58
1.38
0.95
0.83
23.13 1,353
31028127123419411872
21.239.427.426.565.815.654.884.363.522.322.02
9.426.56
4.36
2.322.02
1,078872603
1,057 19.33 165.65 165.6556.74
17.8217.7015.7114.2810.65
8.03
5.595.51
50.3141.77
23.4521.1017.70
10.06
7.746.22
4.263.47
2.69
1.88
1.49
0.93
770612495392345341335297291210162
79
DNA molecular-weight markers that set size standards for linear double-strand DNA. Each One-Step Marker comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel.
Wide Range Ladder (19~22)for wide-range and simultaneous analysis of both DNA size and amount.
DNA molec lar eight markers that set si e standards for linear do ble strand DNA E
Marker & the OneSTEP Marker (23~33)
der (19~22)l i f b th DNA i d tl i f b th
DNA Ladder
DN
A La
dder
-20℃
-20℃
Readyto
use
Readyto
use
Readyto
use
6xLoading
Dye
Readyto
use
-20℃
4
3% Agarose 21
3% Agarose 21
3% Agarose 21
Marker 9
(φX174/HinfⅠ)
Marker 10
(pBR322/MspⅠ)
Marker 11
(pUC19/MspⅠ)
(bp) (bp) (bp)
31 32 33
72671355350042741741331124920015114011810082666648
622527404307242238217201190180160160147147123110907667
501489404331242190147111110
23
24
25
26
27
28
29
30
31
32
33
19
20
2122
DNA Step Ladder Mix (0.08-10 kbp)
Smart Ladder (0.2-10 kbp)
Gene Ladder Wide 1 (0.1-20 kbp)Gene Ladder Wide 2 (0.1-20 kbp)
OneSTEP Marker 1 (lambda/Hind Ⅲ digest)
Marker 1(lambda/Hind Ⅲ digest)
OneSTEP Marker 2 (lambda/Hind Ⅲ + EcoR I double digest)
Marker 2(lambda/Hind Ⅲ + EcoR I double digest)
OneSTEP Marker 3 (lambda/Hind Ⅲ + lambda/EcoR I digest mixture)
Marker 3(lambda/Hind Ⅲ + lambda/EcoR I digest mixture)
OneSTEP Marker 4 (phiX174/Hae Ⅲ digest)
Marker 4 (phiX174/Hae Ⅲ digest)
OneSTEP Marker 5 (phiX174/Hinc Ⅱ digest)
Marker 5 (phiX174/Hinc Ⅱ digest)
OneSTEP Marker 6 (lambda/Sty Ⅰ digest)
Marker 6(lambda/Sty Ⅰ digest)
Marker 7 GT
Marker 8 GT
OneSTEP Marker 9 (phiX174/Hinf Ⅰ digest)
Marker 9 (phiX174/Hinf Ⅰ digest)
OneSTEP Marker 10 (pBR322/Msp Ⅰ digest)
Marker 10 (pBR322/Msp Ⅰ digest)
OneSTEP Marker 11 (pUC19/Msp Ⅰ digest)
Marker 11 (pUC19/Msp Ⅰ digest)
292-68401313-03943317-03941313-06961310-06971310-05251314-05254316-00454312-00456317-05261311-05264319-00564315-00566314-05271310-05273316-00574312-00576318-05791314-05793315-00664311-00666311-05801317-05803312-00674318-00676311-05281317-05283313-00964319-00966315-03981311-03983312-03991318-03993318-05811314-05813314-01491310-01493315-05821311-05823317-01501313-01503312-05831318-05833319-03261315-03263
1 mL25 μL500 μL500 μL × 2500 μL × 21500 μL1500 μL × 5 120 μg 120 μg × 51500 μL1500 μL × 5 80 μg80 μg × 5
1500 μL1500 μL × 580 μg80 μg × 5 375 μL375 μL × 515 μg15 μg × 5375 μL375 μL × 515 μg15 μg × 5
1500 μL1500 μL × 580 μg80 μg × 5 10 μg10 μg × 510 μg10 μg × 5375 μL375 μL × 515 μg15 μg × 5375 μL375 μL × 515 μg15 μg × 5375 μL375 μL × 515 μg15 μg × 5
Code No.Number Product name Package Size Note
Storage conditionsDNA MW Marker :
OneSTEP Marker: Store at 2~10℃
-20℃
: Nippon Gene’s products
5
DGGE MarkerⅠ(5 fragments)
DGGE Marker Ⅱ(10 fragments)
DGGE Marker Ⅲ(10 fragments)
DGGE Marker Ⅳ(8 fragments)
DGGE Marker Ⅴ(9 fragments)
8% Acrylamide
8% Acrylamide
6% Acrylamide
6% Acrylamide
7% Acrylamide
No. of fragments (Conc.)Application
Constant voltageElectrophoresis time, Temp.
5 (50ng/5µL)For bacterial 16S rDNA
10 (100ng/5µL) For bacterial 16S rDNA
10 (100ng/5µL)For bacterial 16S rDNA
8 (80ng/5µL)For fungal bacteria16S rDNA
9 (50ng/5µL)For fungal bacteria 16S rDNA
120V8 hours, 60℃
120V8 hours, 60℃
50V18 hours, 58℃
50V20 hours, 60℃
75V16 hours, 60℃
3
5
1
2
4
Denaturant 35%
65%
Denaturant 35%
65%
Denaturant 50%
70%
Denaturant 20%
Denaturant 20%
45% 50%35 36 37 38
12345678
12345
78910
6
1
2
345
8
67
9
(bp) (bp)105777061249539234534133529729121016279
1005905800702601502398299200101
DNA markers for DGGE (denaturing gradient gel electrophoresis). A CG clamp is added at the end of one side of each fragment to enable separation / inspection by DGGE.
12
43
5678910
M:Marker 5 (φX174/Hinc Ⅱ)39:100L (101 bp, 200 bp, 299 bp, 398 bp, 502 bp)40:100H (601 bp, 702 bp, 800 bp, 906 bp, 1,005 bp)41:200U (101 bp, 299 bp, 502 bp, 702 bp, 906 bp)42:200E (200bp, 398 bp, 601 bp, 805 bp, 1,005 bp)43:Mixture of products from a 100L & 100H PCR
0.5 µg3 µL3 µL3 µL3 µL
3 µL each
DNA markers for DGGE (dena
DGGE Marker (34~38)
for preparing DNA size markers. Consisting of a mixture of template DNA, primer, and reaction buffer, the reagent initiates a PCR with the simple addition of a Tag DNA polymerase “Gene Tag.” It can also be used as a positive control while the PCR is under way.
for preparing DNA size markers Consisting of a mixt
PCR Marker Maker (39~42)
aturing gradient gel electrophoresis) A CG clamp is aaturing grad
4~38)
DNA Ladder
DN
A La
dder
34
-20℃ -20℃ -20℃ -20℃ -20℃
-20℃
34
35
36
37
38
39
40
41
42
DGGE Marker Ⅰ (5 fragments)
DGGE Marker Ⅱ (10 fragments)
DGGE Marker Ⅲ (10 fragments)
DGGE Marker Ⅳ (8 fragments)
DGGE Marker Ⅴ (9 fragments)
PCR Marker Maker 100L PCR Marker Maker 100L, Gene Taq attachedPCR Marker Maker 100HPCR Marker Maker 100H, Gene Taq attachedPCR Marker Maker 200U PCR Marker Maker 200U, Gene Taq attachedPCR Marker Maker 200E PCR Marker Maker 200E, Gene Taq attached
318-06411314-06413312-06414311-06401317-06403315-06404315-06921311-06923319-06924312-06931318-06933316-06934319-06941315-06943313-06944317-03061314-03071311-03081318-03091318-03111315-03121312-03131319-03141
30 μL (for 6 applications)150 μL (for 30 applications)150 μL × 5 (for 150 applications)30 μL (for 6 applications)150 μL (for 30 applications)150 μL × 5 (for 150 applications)30 μL (for 6 applications)150 μL (for 30 applications)150 μL × 5 (for 150 applications)30 μL (for 6 applications)150 μL (for 30 applications)150 μL × 5 (for 150 applications)30 μL (for 6 applications)150 μL (for 30 applications)150 μL × 5 (for 150 applications)For 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applications
Code No.Number Product name Package Size Note
39 40 41 42 43M
: Nippon Gene’s products6
aa b c d e f b c d e f
Xylene Cyanol FF
Bromophenol Blue
Orange G
1% formaldehyde Agarose
5% polyacrylamide-urea
1% formaldehyde Agarose
1% formaldehyde Agarose
RNA Size Standard
Marker ⅡRNA Size Standard
Marker Ⅲ (0.1-1 kb)
RNA Size Standard
Marker Ⅳ (0.28-6.58 kb)
RNA Ladder(0.125-6 kb)
RNA Ladder
1 2 3 4
9
750500400
300
200
100
(k base) (base)
1,000
(base)
6,583
(base)
6,0004,0003,0002,0001,5001,000500250125
4,9813,638
2,604
1,908
1,383
623
281
955
654
32.52
1.5
1
0.5
RNA Size Standard Marker (1~4) RNA molecular-weight markers prepared by in vitro transcription.
RN
A La
dder
-80℃ -70℃-70℃-70℃
2% Native Agarose
2% Formaldehyde Agarose
A sample can be easily applied during DNA gel electrophoresis to effectively predict the migration point.
Loading Buffer
1 % agarose S2 % agarose S3 % agarose 21
100 bp (approx.) 30 bp (approx.) 10 bp (approx.)
900 bp (approx.)300 bp (approx.)100 bp (approx.)
5,000 bp (approx.)1,500 bp (approx.) 500 bp (approx.)
Orange G Bromophenol Blue Xylene Cyanol FF
Marker 4(φX173/Hae Ⅲ)Product PCR(299 bp) 50 ng
TE Buffer
6 × Loading Buffer Triple Dye6 × Loading Buffer Orange G6 × Loading Buffer Triple Dye6 × Loading Buffer Orange G6 × Loading Buffer Triple Dye6 × Loading Buffer Orange G
abcdef
100
200
300400
6008001,000
100
200
300400
6008001,000
(base) (base)
RNA Step Ladder (0.1-1 kb)
5
123
4
5----
RNA Size Standard Marker Ⅱ (0.5-9 kb) RNA Size Standard Marker Ⅲ (0.1-1 kb)RNA Size Standard Marker Ⅳ (0.28-6.58 kb)
RNA Ladder (0.125-6 kb)
RNA Step Ladder (0.1-1 kb)Loading Buffer6 × Loading Buffer OrangeG6 × Loading Buffer Double Dye6 × Loading Buffer Triple Dye
542-00651545-01621188-01831311-06261317-06263298-61301313-90111317-90251313-90351314-90261
50 μg50 μg50 μL25 μL25 μL × 240 μL × 510 mL1 mL × 31 mL × 31 mL × 3
Code No.Number Product name Package Size Note
: Nippon Gene’s products
(Store at room temp.)
-80℃
an RNA molecular-weight marker can be directly applied to native gel, denatured gel, or polyacrylamide gel.
RNA l l i ht k b di tl li d
RNA Step Ladder (0.1–1 kb) (5)
2XLoading
Dye
7
Electrophoresis-related ProductsProtein Size Marker
Prestained PrestainedⅡ Western
For low molecularrange
For middle molecularrange
For high molecularrange
Wide range
Biotinylated1 2 3 4
5 6 7 8
(kDa) (kDa) (kDa)
(kDa) (kDa)(kDa)(kDa)
(kDa)
30
20
15
140
100
70
50
40
79
42
31
21
14
42
30
20176.53.5
200
116
79
42
30
17
1801169779
42
30
20
146.5
79
42
30
2014
150120100
80
60504235
25
16
240140100
70
50
35
2520
15
7
Contains protein G with the ability to combine with the Fc region of immunoglobulin. Reacts with both primary and secondary antibodies in a western blot.
Prestained Marker (1, 2) :
Contains biotinylated protein. Capable of both chromogenetic and photogenetic detection by reacting with avidin HRP or avidin AP.Biotinylated Marker (4) :
Prote
in S
ize M
arker
Western Marker (3) :
With dye combined in its recombinant protein content.
-20℃ -20℃ -20℃
Marker for CBB Dyeing (5~8)Optimized for CBB dyeing. Obtains sharp, uniform bands because of a reduction alkyl process.
12345678
WIDE-VIEW TM Prestained Protein Size MarkerWIDE-VIEW TM Prestained Protein Size Marker 2WIDE-VIEW TM Western Size MarkerWIDE-VIEW TM Biotinylated Size MarkerMolecular Weight Marker, Low Range Molecular Weight Marker, Middle RangeMolecular Weight Marker, High RangeMolecular Weight Marker, Wide Range
230-02221239-02291233-02211233-02331294-63101131-14511134-14501296-63301
500 μL500 μL250 μL 1.5 mL1 mL1 mL1 mL1 mL
Wako Cat. No.Number Product name Package Size
Store at ー20℃Store at 2~10℃
:
NO DISPLAY:
-20℃
8
Easy Set Easy Remove
1 2 3
a
b
c
d
rat muscle glycogen phosphorylase (EC2.4.1.1)
relative abundance
Electrophoresis-related ProductsElectrophoresis-related Products
Easy to operate.
Requires only a small amount (about 250-350ml) of running buffer for electrophoresis.
Allows the gel plate to be removed without removing the running buffer after electrophoresis has been completed.
Features
12
3
Detectable limit of protein: 1 ng
Sensitive and Simple procedure with 2-step staining in 5~10 minutes
Optimized the staining intensity by either de-staining the excess stain or re-staining following de-stain of the gel.
Applicable to proteome analysis by mass spectrometry and sequencing
Featureas
12
3
4
Easy SeparatorTM (Wako Catalog No.058-07681 (1 set ) )
Easy SeparatorTM is an electrophoresis tank specially designed for “SuperSep” products to enable easy setting and removal of the gel plate.
Negative Gel Stain MS KitProtein bands separated by SDS/polyacrylamide gel electrophoresis (SDS-PAGE) can be visualized as stable, clear and transparent bands against the background of milky white gel stained by negative gel stain containing our improved imidazol derivative reagent in as little as 10 minutes. The staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues for the subsequent studies of protein such as sequencing and mass analysis of peptide.
①Remove the cover after electrophoresis has been completed.
①Insert a gel. ②Turn the knobs toward you.
③Setting is completed.
②Turn the knobs away from you.
③Remove the gel without removing the running buffer.
Electr
ophoresis
-rela
ted P
roducts
Negative Gel Stain MS KitKit Contents:1) Staining S olution A (1 × 500 mL); 2) Staining Solution B (1 × 500 mL); 3)De-staining Solution (1 × 500 mL)
293-57701 20 tests
Wako Cat. No. Product name Package Size
SDS-PAGE analysis
MALDI-TOF/MS analysis
Figure 1. SDS-PAGE analysis of proteins using Negative Gel Stain MS Kit
a : rat phosphorylase (97k);b : bovine serum albumin (68k);c : hen egg white ovalbumin (45k);d : bovine carbonic anhydrase (31k).Lane 1: 50 ng; Lane 2: 100 ng; Lane 3: 150 ng
SDS-PAGE gel
Mass Spectrometry
Staining Sol. A
Staining Sol. B
Shake 5 ~ 10 min.Wash 5 ~ 10 sec. x 3
Shake 10 ~ 60 sec.Wash 1 min. x 3
Check staining intensityof the gel against a sheet of black paper
Procedure
Figure 2.
MALDI-TOF/MS of rat phosphorylase
The band was excised and treated with trypsin. Following the in-gel digestion and preparation, the sample was analyzed on MALDI-TOF mass spectrometer. (These data were provided by Dr. Y. Wada at Osaka Medical Center, Japan.)
1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000m/z
(Store at room temperature)
9
SuperSepTM SuperSepTM Ace
17well13wellSuperSepTM AceSuperSep™ Ace is a polyacrylamide gel of the highest quality, designed to resolve the problems of existing products (SuperSep™ and SuperSep™ HG). Tris-glycine buffer is used in the gel buffer, similar to existing products, but because its pH is neutralized, the gel’s chemical storage stability is improved. Physical storage stability is also improved by using vacuum packaging. And because it does not contain SDS, it can be used in SDS-PAGE and Native-PAGE by changing the running buffer.
① SuperSepTM: 10%
②SuperSepTM Ace: 10%
Plate size
Gel size
Number of wells
Well volume
Total amount of protein* (/well)
13 wells
30 μL
3.3~ 6.5 μg
17 wells
25 μL
1.3~ 3.9 μg
100 × 100 × 3 (mm)90 × 85 × 1 (mm)
Product specifications
*Indicates total amount of protein that can be clearly separated.
Good separation and uniform bands
Can be used with a multi-channel pipette.
Reasonably priced and capable of long-term storage (for 6 months)
Box sticker allows easy identification of type of gel.
Comes in 13-well and 17-well types.
12345
0 1.5 3 4.5 6 7.5 9 10.5 12 (hours)
200
(kDa)
150
10075
50
37
25
20
Improved
separation
near the center!Improved
separation
near the center!
17well13wellSuperSepTM AceSuperSep™ Ace amide gel of the highest qualityis a polyacryla
Electrophoresis-related ProductsElectrophoresis-related Products
Electr
ophoresis
-rela
ted P
roducts
Features
Uniform
band!
Convenient
sticker for
gel
identification!
Convenient
sticker for
gel
identification!
SuperSepTMAce, 6%, 13well SuperSepTMAce, 6%, 17well SuperSepTMAce, 7.5%, 13well SuperSepTMAce, 7.5%, 17well SuperSepTMAce, 10%, 13well SuperSepTMAce, 10%, 17well SuperSepTMAce, 12.5%, 13well SuperSepTMAce, 12.5%, 17well SuperSepTMAce, 15%, 13well SuperSepTMAce, 15%, 17well SuperSepTMAce, 5-12%, 13well SuperSepTMAce, 5-12%, 17well SuperSepTMAce, 5-20%, 13well SuperSepTMAce, 5-20%, 17well SuperSepTMAce, 10-20%, 13well SuperSepTMAce, 10-20%, 17well SuperSepTMAce, 15-20%, 13well (Tricine Gel)
195-15171192-15181198-14941191-14931195-14951192-14961199-14971196-14981193-14991190-15001199-15191192-15201197-15011194-15021191-15031198-15041198-15301
10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.
Wako Cat. No. Product name Package Size
(Store at 2~10℃)
10
20-200 bp DNA was clearly separated by using SuperSepTM DNA and Tris-glycine buffer. PCR products under 200 bp can be used for separation from gel, especially when cloning small RNA. 30-200 bp DNA was clearly separated with TBE buffer.
Features
(bp) (bp) (bp) (bp)
1009080706050403020
200150
25
(bp) (bp)
100
50
30
20
EtBr staining EtBr staining
100
200 200150
100
75
50
25
150
100
75
50
25
90
80706050
40
30
20
(bp)
100
50
3020
200150
25
(bp)
1% Agarose SuperSepTM DNA(15%, 17 well)
SuperSepTM DNA(15%, 17 well)
2.5% Agarose
Example of electrophoresis
Improved
separation
near the center!
1
3
2
The acrylamide concentration, degree of cross-linkage, and number of wells are optimized for the electrophoresis of low-molecular-weight DNA.
Cleaner separation image than that obtained with agarose gel.
Ideal for 20-200 bp separation.
Plate size
Gel size
Number of wells
Well volume
100 × 100 × 3 (mm)
90 × 85 × 1 (mm)17 wells
25 μL
Product specifications
①With Tris-Glycine running buffer ②With TBE running buffer
SuperSepTM DNA (15%, 17 wells) (Wako Catalog No.190-15481 (10 ea.) )
Polyacrylamide gel is used in the separation of low-molecular-weight DNA because it has a finer gel mesh structure than agarose. However, the DNA size will vary widely depending on the acrylamide concentration and the degree of cross-linkage of the gel. In SuperSepTM DNA, the acrylamide concentration and the degree of cross-linkage of the gel have been optimized for the separation of low-molecular-weight DNA. This product is therefore effective for the separation of samples with a DNA size from 20-200 bp.
Electrophoresis-related Products
Convenient
sticker for
gel
identification!
EtBr Solution 315-90051 10 mLWako Cat. No.Product name Package Size
(Store at 2~10℃)
Staining Reagent
11
Store at or below ー20℃
【How to read the chart】 【Display codes】
10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp
DNA Ladders
10000 b 1000 b 100 b 10 b
10000 b 1000 b 100 b 10 b
Protein Size Markers 10 kDa 100 kDa 1000 kDa
10 kDa 100 kDa 1000 kDa
●1 , p2
●1 , p1
●2 , p2●3 , p2
●6 , p2●7 , p2●8 , p2
●1 , p7●2 , p7
●5 , p7
●3 , p7
●1 , p8●2 , p8
●3 , p8●4 , p8
●5 , p8●6 , p8
●7 , p8●8 , p8
10 b
RNA Ladders
●10 , p2●9 , p2
●11 , p2●12 , p2
●17 , p3●18 , p3
●13 , p3●14 , p3
●15 , p3●16 , p3
●19 , p4●20 , p4
●21 , p4●22 , p4
●26 , p4●27 , p4
●23 , p4●24 , p4●25 , p4
●28 , p4●29 , p4●30 , p4
●31 , p5●32 , p5●33 , p5
●40 , p6●41 , p6●42 , p6
●39 , p6
●4 , p7
【How to read the chart】 【Display codes】Selection Guide
Number Reference page
Store at 2~10℃NO DISPLAY Store at or below ー80℃
Store at or below ー70℃
-80℃
-70℃-20℃
●4 , p2●5 , p2
10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp
08X05開01
co.jpco.jp
09Y3IBF
・This brochure may contain products that cannot be exported to your country due to regulations.・Listed products are intended for laboratory research use only, and not to be used for drug, food or human use.
top related