microbial methanol synthesis team 3: robert clifford, patricia firmin, gen liang, rooma raza
Post on 16-Dec-2015
222 Views
Preview:
TRANSCRIPT
Methanotroph Groupings
Group I: Ribulose Monophosphate Cycle Group II: Serine Cycle
Inhibit Enzyme MDH
Optimize Enzyme MMO
Advantages of using ammonia-oxidizing bacteria (AOB)
• Oxidize methane to methanol via the nonspecific action of the
enzyme ammonia monooxygenase
• Contaminants such as moisture and CO2 do not post a limitation for
biological conversion
• Can utilize the CO2 contained in gas mixtures for cell synthesis
Methanol Production by AOB
• Methanol production rate varies for conditions and performance
• Maximum specific productivity is 0.82 mg methanol/ mg biomass
(COD)/d
Industrial Challenges
• Microorganisms are limited.
• Inhibition on cell growth by H2S when methane in biogas
is used.
• High-cost electron donors required for conversions
• Gas-Liquid Mass transfer limitations
• NH3 may inhibit the growth of microorganisms including
methanotrophs
Changes in the reactor design by using Trickling biofilters for enhanced methane
supply
Methanotrophic strains from AD systems
Genetic engineering of ANME
Microbial electrosynthesis
Potential Strategies
References
• Fei, Q. G., Michael. Tao, Ling. Laurens, Lieve. Dowe, Nancy. Pienk, Philip T.os. (2014). Bioconversion of natural gas to liquid fuel: Opportunities and challenges. 32(3), 596–614. doi: 10.1016/j.biotechadv.2014.03.011
• Ge, X., Yang, L., Sheets, J. P., Yu, Z., & Li, Y. (2014). Biological conversion of methane to liquid fuels: Status and opportunities. Biotechnology Advances, 32, 1460-1475. doi: - http://dx.doi.org/10.1016/j.biotechadv.2014.09.004
• Pen, N. S., L. Belleville, M.-P. Sanchez, J. Charmette, C. Paolucci-Jeanjean, D. (2014). An innovative membrane bioreactor for methane biohydroxylation. Bioresource Technology, 174, 42–52. doi: 10.1016/j.biortech.2014.10.001
• Thorn, G. J. S. (2005). Development of an Immobilized Nitrosomonas europaea Bioreactor for the Production of Methanol from Methane - thesis_fulltext.pdf. Department of Chemical Process Engineering. University of Canterbury. Retrieved from
http://ir.canterbury.ac.nz/bitstream/10092/1867/1/thesis_fulltext.pdf
top related