langmuir technology. stm photo of langmuir film of stearic acid c 17 h 35 cooh
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Langmuir technology
Langmuir technology
STM photo of Langmuir film of Stearic AcidC17H35COOH
Langmuir technology
Type of polymer
Area per monomeric chain containing cetyl-radical, A2
Area per general monomeric chain, A2
abPEI-12 alPEI-35 alPEI-70 abPEI-70 aP4VP-70
cLMMA-25 cLMMA-40 cLMMA-90
31.0 50.0 32.0 55.0 17.0 76.0 56.0 28.0
6.2 14.9 23.4 37.9 11.4 19.6 19.2 20.0
Schemas of STM and AFM a – scanning tunneling microscopyb – atomic force microscopy
AFM images of Langmuir films of polymers
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Langmuir film of antibodies with polymer PEI-R12 on graphite surface
Langmuir film of antibodies with polymer PEI-L70 on graphite surface. AFM image, size 2,6x2,6 μ.
AFM images of Langmuir films of polymers
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a b
c d
AFM images of Langmuir films of polymer PEI-R12, size1,51,5 μ.
a – surface pressure by LB process í=10 mN/m, thickness of film 2,5 nm;
b – í=20 mN/m, thickness of film 3,5 nm;
c – í=30 mN/m, thickness of film 3,5 nm;
d – í=40 mN/m, thickness of film 3,5 nm.
AFM images of Langmuir films of polymers
AFM images of Langmuir films of polymer PEI-L70, size1,51,5 μ.
a – surface pressure by LB process í=10 mN/m, thickness of film 3,3 nm;
b – í=20 mN/m, thickness of film 4 nm;
c – í=30 mN/m, thickness of film 5,5 nm;
d – í=40 mN/m, thickness of film 6 nm.
a b
c d
STM images of DNA
DNA molecules on graphite surface
AFM images of viruses
Sample of rotaviruses specific adsorbed on Langmuir film from antibodies with polymer PEI-R12. AFM image, 3D projection with highlight.
Viruses of Variolovaccine on mica surface
Cells of Rickettsia bacterium C. burnetii (culture ”Yellow throat mouse -Luga”) on Langmuir film from antibodies with polymer PEI-L70. 3D projection with highlight.
Cells of bacterium Rickettsia prowazekii (culture ”Breil”) on Langmuir film from antibodies with polymer PEI-R12. 3D projection with highlight.
Cells of bacterium Rickettsia bacterium C. burnetii (culture ”M-44”) onmica. 3D projection with highlight.
AFM images of bacteriums
Cells of bacterium Yersinia pestis (culture ”EB”) on Langmuir film from antibodies with polymer PEI-R12. 3D projection with highlight.
AFM images of bacteriums
ASM images of Bacillus subtilis
AFM images of bacteriums
AFM images of bacteriums
ASM images of Staphylococcus aureus 209-P
AFM images of bacteriums
Bacteriums Staphylococcus aureus on glass. ASM image with highlight.
Bacteriums Staphylococcus aureus, treated for 15 min by enzyme lysoamidaze. ASM image with highlight.
Investigations of 2D crystalline films of ferritin
1- initial electronmicroscopic photo 2 - optical diffraction 3 - after Fourier filtration
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2
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Investigations of 2D crystalline films of protein
Self-organized protein of S-layer of Bac.spaericus cell wall with tetragonal packing of sub-units. TEM, contrast by 1% uranyle-acetate.
1 - fragment EM photo,
2 - optical diffraction pattern,
3 - filtered image
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23 1
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