introduction to microbiology course outline 01
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Microbiology I
Microbiology I
IntroductionFundamental Skills for the Microbiology
LaboratoryMicrobial GrowthMicroscopy and Staining
Introduction
Safety and Laboratory GuidelinesStudent ConductBasic Laboratory SafetyReducing Contamination of Self, Others,
Cultures, and the Environment
Introduction
Disposing of Contaminated MaterialsA Word About Experimental Design
Fundamental Skills for the Microbiology LaboratoryComparison of Hand-Cleaning AgentsGlo Germ Hand Wash Educational SystemBasic Growth MediaAseptic Transfers and Inoculation Methods
Basic Growth Media
Nutrient BrothNutrient Agar
Aseptic Transfers and Inoculation Methods
Common Aseptic Transfers and Inoculation MethodsTransfer from a broth culture to a sterile brothTransfer from a slant culture to a sterile agar slantTransfers from plate culture to sterile broth
Streak Plate Methods of Inoculation
Aseptic Transfers and Inoculation Methods
Inoculation of agar plates using the quadrant streak Method
Inoculation of agar plates with a cotton swab in preparation for a quadrant streak plate
Spread Plate Method of Inoculation
Microbial Growth
Diversity and Ubiquity of MicroorganismsEnvironmental Factors Affecting Microbial
GrowthAerotoleranceControl of Pathogens and Chemical Methods
Diversity and Ubiquity of Microorganisms
Ubiquity of MicroorganismsColony MorphologyGrowth patterns on slantsGrowth patterns on broth
Environmental Factors Affecting Microbial Growth
Evaluation of Media
Aerotolerance
Fluid thioglycollate MediumAnaerobic jarEffects of temperature on microbial growthEffects of pH on microbial growthThe effect of osmotic pressure on microbial
growth
Control of Pathogens and Chemical Methods
Steam SterilizationThe lethal effect of ultraviolet radiation microbial
growthChemical germicides disinfectants and
antiseptics
Microscopy and Staining
Introduction to light microscopyCalibration of the ocular micrometerMicroscopic examination of eukaryotic microbesBacterial Structure and simple stains
Simple stainsNegative stains
Microscopy and Staining
Differential and structural stainsGram StainAcid-Fast StainCapsule StainEndospore stainBacterial Motility
Wet Mount Hanging Drop Preparation Flagella Stain
Morphological Unknown
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