h.ghaderian1 1-deparyment of microbiology, faculty of biological sciences, islamic azad univercity,...

H.Ghaderian1

1-Deparyment of Microbiology , Faculty of Biological Sciences , Islamic Azad Univercity , Falanarjan Branch , 804515/155 , Esfahan , Iran

: Classification

Family : Herpes viridea

Sub family : herpes virinea

Genus : simplex Specieas:HSV-1CL 101 ,HSV-2 strain KN 53690

common name : herpes symplex virus

tegument

Alpha herpes viruses

latently infected

Herpes virus in the host cell

HSV of the newborn is acquired 3 periods :

in utera

Peripartium (85%)

postpartum

Incidence of neonatal herpes

in 3 form :

1 )lesion of the skin , eyes , mouth

2 )Encephalitis with or without lesion

3 )Dissiminated desease in liver

lunges

gland adrenl

gut

Neonatal herpes

o case of neonatal herpes HSV -1and HSV-2

o Investigation showed Neonatal disseminated HSV infection is most frequently caused by HSV-2, although HSV-1 can also be the cause

o Serologic investigations showed no IgM and IgG antibodies for HSV-1 and HSV-2 in new born

Immune ResponsesNeonatal

Infected neonates produce HSV-specific IgM antibodies within 3 weeks of acquisition of the viral infection ,

which increase rapidly during the first 2 to 3 months

and may remain for as long as 1 year after neonatal infection develops.

Lymphocytes from infected infants

alpha-interferon

Neonates INF –αcomparewith adults with primary HSV infection is decreased.

The first time infection of the pregnant women may lead to severe illness in pregnancy and

may be virus transmission to newborn.

Study showed:

DIAGNISTIC

The high risk for death requires prompt diagnostic therfore suggestive that of HSV was identified by PCR

Viral DNA was extracted from all specimens(tracheal aspirate, liver, lungs, and gut)

By using the QIAamp DNA Mini Kit

1)PCR

primers for the HSV-1: thymidinekinase gene

((Fw 5′-AGCGTCTTGTCATTGGCGAA-3′

(Rev 5′-TTTTCTGCTCCAGGCGGACT-3′)

Primer for the HSV-2: DNA polymerase gene

((Fw 5′-CGTCCTGGAGTTTGACAGCG-3′

(Rev 5′-CAGCAGCGAGTCCTGCACACAA-3′)

The DNA was then used for HSV DNAPCR

Nucleotide sequence analysis

showed identical sequences in different specimens.

GenBank BLAST tool

highest similarity was

HSV-1 strain CL 101 and HSV-2 strain KN 53690

2)Cell culture

•Multi nucleated epithelial cells

treatment All infants with diagnosed HSV infectionmust be treated with an intravenous therapy with acyclovir

(60 mg/kg/day)

Antibody Therapy

Antibodies against the surface glycoproteins gB and gD prophylactic and therapeutic agents in HSV infection

inactive acyclovir

Viral thimydin kinas

Monophosphte acyclovoir

cellular thimydin kinase

active )) acyclovir three phosphate

mechanism acyclovir

is analog to guanin blocked DNApolymerasefunction

PREVENTION

Cesarean Deliverycesarean delivery in a woman with activegenital lesions can reduce risk of acquiring HSV to infant

Antiviral Prophylaxis During Pregnancythe use of oral acyclovir near the end of pregnancy to suppress genital HSV Because of acyclovir’s safety

HSV-2 : glycoprotein D subunit

vaccine adjuvanted :monophosphorylipid A(MPL)

Hsv vaccine

efficacy was limited to women who were HSV-1 and -2 seronegative before receiving vaccination.

Thank you for your Attention