fabrication of biosensors and lab testings iii

Jack Nguyen Xiaodong Ma

05/14/2008

For todayFor todayTheory/Background, Testings – Jack

Result, other two experiments– Xiaodong

OBJECTIVES OF PROJECT:OBJECTIVES OF PROJECT:Fabrication of FP Biosensors Repeated Analyze antigen/antibody (Influenza

A/anti-Influenza A ) interactionsTest different Techniques / Antigens

MATERIALSMATERIALS::Fiber Optic Strands, CTS Machine, Matlab,

PMMA/Acetone solution, Protein G’(10µg/ml), 2% BSA in PBS, Influenza A(20µg/ml), Anti-Influenza A Ig G(20µg/ml), Distilled water, 3-aminopropyltriethoxysilane, Acetone, Ethanol, Microscope, Light, Controllable stage

BudgetBudget: $0, all materials provided by UML

Team RolesTeam RolesTASKS RESPONSIBILITY

Lab Reports / PPTLab Reports / PPT Jack, XiaodongJack, Xiaodong

Fabrication of BiosensorsFabrication of Biosensors JackJack

Lab TestingsLab Testings Jack, XiaodongJack, Xiaodong

Data AnalysisData Analysis XiaodongXiaodong

Schedule FollowedTASKS START END

Sensors Fabrication 4/01/08 5/13/08

Repeat Lab Testings 4/01/08 5/13/08

Present. II/Report 4/09/08 4/16/08

Present.III/Report 4/16/08 5/14/08

Data Analyzation 4/10/08 5/12/08

Test Diff. Antigens 4/30/08 5/13/08

Analyze Diff. Anti. 5/02/08 5/13/08

Finalization All Testings 5/10/08 5/14/08

Principle of FP cavity sensor.

Schematic diagram showing the fringe shift

Pro.G

Influenza A

Primary Antibody

poly(methyl 2-methylpropenoate)

Optical Fiber

Fabrication of Biosensor

SENSOR PMMA PBS

BSA WATER Pro.G

WATER PBS IgG

Inf.A PBS WATER

WATER PBS DATA

Time DurationFabrication FP sensor : 30 MinutesPMMA: 2 HoursPro.G: 1.5 HoursBSA: 1.5 HoursIgG: 1.5 HoursInflu. A: 1.5 HoursTotal PBS: 5 HoursTotal Hours for a typical lab test: 13-16 HrWhen a lab test fails, we start all over with the

FP sensor fabrication

Experimental setup

Chiller

CTS

MatlabProgram

Biomaterials Fiber

Experimental setup

Polymertube

Glasstube

Fiber

Fringes in the air

Fringes in the PBS

Result I without BSA

1537

1539

1541

1543

1545

2:18-3:55p.m. 8:00-8:31p.m. 10:27-10:57p.m. 1:00-1:33a.m.

Pro.G Anti-Inf.A IgG

Inf.A

PBS

Time

Wave Length

Result II without BSA

1540

1542

1544

Wave Length

10:52-11:15a.m. 12:34-1:05p.m. 2:27-3:00p.m. Timeovernight

Pro.G

Anti-Inf.A IgG

Inf.A

PBS

Result III with BSA

1534

1536

1538

1540PBS

Pro.G

Pro.G_PBS

BSA

BSA_PBS

Anti-Inf.A IgGAnti-Inf.A IgG_PBS Inf.A

Inf.A_PBS

1.5hr 1hr 1.5hr 1hr 1.5hr 1hr 1.5hr 1hr0.5hr

Wave Length

Time

Experimental setup

Stage power

Connector to CTS

Detector

Stage

Sensor

Result

Experimental setup

VIS Spectrometer

Detector

Light Source

SiO2/Si

Experimental steps• Wash out the dust of SiO2/Si substrates several times by DI water.• Wash the SiO2/Si substrates by sonication in acetone, ethanol and DI water for 15

min, respetively.• Put the cleaned and dry SiO2/Si substrates in 2% solution of 3-

aminopropyltriethoxysilane in acetone (3-aminopropyltriethoxysilane : acetone =1 : 49 by volume ) at room temperature for 30 seconds.

• Measure the substrates’ spectra by VIS spectrometer after SiO2/Si substrates dried.• Drop Pr.G’ on SiO2/Si substrates for 1.5 h, wash by DI water 3 times, air-dry and

detect its spectra. (For comparison experiment, drop BSA on the substrates first and then wash and detect spectra.)

• Drop anti-Inf.A IgG on the surface for 1.5 h, wash by DI water 3 times, air-dry and test its spectra. (For comparison experiment, drop Pr.G’ on the surface with BSA and wash, dry detect spectra.)

• Drop Inf.A on the surface for 1.5, wash by DI water 3 times, air-dry and then measure its spectra.

• Analyze the spectra of two groups experiment.

Results

0

1000

2000

3000

4000

5000

6000

7000

8000

9000

10000

459

479

498

518

537

555

573

591

609

626

643

660

Substrate

Pr.G'

Anti-Inf.A IgG

Inf.A

Relative Intensity

Wavelength (nm)

A

Results

-800-600-400-200

0200400600800

10001200

459

474

489

504

519

533

547

562

576

589

603

616

629

642

655

668

P-S

A-P

I-A

Relative Intensity

Wavelength (nm)

B

Results

0

2000

4000

6000

8000

10000

12000

14000

459

473

486

500

513

526

539

552

565

577

589

602

614

626

637

649

660

Substrate

BSA

Pr.G'

Relative Intensity

Wavelength (nm)

A

Results

-2000

-1500

-1000

-500

0

500

1000

1500

2000

2500

3000

459

473

486

500

513

526

539

552

565

577

589

602

614

626

637

649

660

B-S

P-B

Relative Intensity B

Wavelength (nm)