droplet gradient array for parallel experimentation via microfluidic device lindsay pickett faculty...

Droplet Gradient Array For Parallel Experimentation via Microfluidic Device

Droplet Gradient Array For Parallel Experimentation via Microfluidic Device

Lindsay PickettFaculty Mentor: Abraham P. Lee

Graduate Mentor: Rob LinIM-SURE 2006

Lindsay PickettFaculty Mentor: Abraham P. Lee

Graduate Mentor: Rob LinIM-SURE 2006

Outline of DiscussionOutline of Discussion

IntroductionGoals

ProcessResultsIssues

SolutionsFuture Direction

IntroductionGoals

ProcessResultsIssues

SolutionsFuture Direction

IntroductionMicrofluidic Devices

IntroductionMicrofluidic Devices

• Widely applicable- engineering, biology, chemistry– Research tools– Commercial applications

• Advantages: – microscale fluid physics– less reagent & shorter Rxn time – parallel operations – lab space conservation,– easy/cheap fabrication

• Widely applicable- engineering, biology, chemistry– Research tools– Commercial applications

• Advantages: – microscale fluid physics– less reagent & shorter Rxn time – parallel operations – lab space conservation,– easy/cheap fabrication

Project GoalProject Goal

Droplet Gradient Array

Ismagilov Laboratory– Thorough characterization– Control over droplet properties

• Droplet volume•Gradient steps

– Versatility of device for multiple applications

Droplet Gradient Array

Ismagilov Laboratory– Thorough characterization– Control over droplet properties

• Droplet volume•Gradient steps

– Versatility of device for multiple applications

Project OutlineProject Outline

Process: Device designFabrication TestingExternal valve

systemComputer-

mediated step gradient

Characterization

Process: Device designFabrication TestingExternal valve

systemComputer-

mediated step gradient

Characterization

QuickTime™ and a decompressor

are needed to see this picture.

Device DesignDevice Design

Outlet reservoir

Reagent Inlets

Oil Inlet

Shearing JunctionArray Observation Region

Process: Device designProcess: Device design

12 µm

7.5 µm

70/30 µm 50/50 µm 12/7.5 µm12/7.5 µm

Process: Experimental SetupProcess: Experimental Setup

Results:Testing

Results:Testing

• Top Aq. Inlet: DI (variable rate)

• Bottom Aq. Inlet: DI + dye (const. rate)

• Oil inlet: Silicon oil (const. rate 2µL/min)

*Droplet content control*

• Top Aq. Inlet: DI (variable rate)

• Bottom Aq. Inlet: DI + dye (const. rate)

• Oil inlet: Silicon oil (const. rate 2µL/min)

*Droplet content control*

0.80 vs 0.70 µL/min

0.95 vs 0.70 µL/min

1.25 vs 0.70 µL/min

QuickTime™ and a decompressor

are needed to see this picture.

Results: Array Capture (Colorcam)

Results: Array Capture (Colorcam)

• Array image capture external valves work• Not pictured instability introduced by valves

– Initializing system: backflow, fluctuation of flow rate– Over 20 min observation period: chaotic, random instability

• Array image capture external valves work• Not pictured instability introduced by valves

– Initializing system: backflow, fluctuation of flow rate– Over 20 min observation period: chaotic, random instability

Solutions…Solutions…

VALVES• Contacted vendor…

– no suitable suggestions

– different fluid route (Dr. Lee)

• Different External Valves• Quake pressure-

actuation Valves• 2-D valve for sufficient

freeze

VALVES• Contacted vendor…

– no suitable suggestions

– different fluid route (Dr. Lee)

• Different External Valves• Quake pressure-

actuation Valves• 2-D valve for sufficient

freeze

QuickTime™ and aTIFF (Uncompressed) decompressor

are needed to see this picture.

QuickTime™ and aTIFF (Uncompressed) decompressor

are needed to see this picture.

Other Solutions…Other Solutions…

*Fluid re-routing…*new filtration design… *input tips*Fluid re-routing…*new filtration design… *input tips

Future DirectionFuture Direction

• Valves/new designs• Computer-mediated

step gradient (Hyperterminal)

• Characterization via GFP and ImageJStep gradientDroplet volume

• Valves/new designs• Computer-mediated

step gradient (Hyperterminal)

• Characterization via GFP and ImageJStep gradientDroplet volume

AcknowledgementsAcknowledgements

• NSF and the IMSURE program• Abraham P. Lee (faculty mentor)• Rob Lin (grad. student mentor)• Lee laboratory team• Eckerd College Biochemistry Dept.

• NSF and the IMSURE program• Abraham P. Lee (faculty mentor)• Rob Lin (grad. student mentor)• Lee laboratory team• Eckerd College Biochemistry Dept.