dna fingerprinting

DNA FINGERPRINTING

The story…The story…

1) Provide a timeline1) Provide a timeline

2) Draw a family tree2) Draw a family tree

3) How could you explain Tom’s 3) How could you explain Tom’s resemblance to TJ?resemblance to TJ?

4) Why is the Y chromosome a good 4) Why is the Y chromosome a good marker?marker?

5) What is a haplotype?5) What is a haplotype?

6)How can the descendants be used to 6)How can the descendants be used to indicate paternity?indicate paternity?

DNA

• Double helix

• A = T C = G

Restriction Enzymes

• Bacterial origin• Cuts DNA sequences into shorter fragments• Site specific

Ex: EcoR1 cuts at GAATTC sequence

different DNA sequences

Living organisms that have different characteristics

The more different the organisms

the more different are the DNA sequences

Restriction EnzymesRestriction Enzymes

www.uic.edu/classes/phar/phar331/lecture6/

DNA FINGERPRINTINGDNA FINGERPRINTING

http://www.biotech.iastate.edu/biotech_info_series/bio6.html#anchor861530#anchor861530

Professor Sir Alec Jeffreys

DNA Fingerprinting-DNA Fingerprinting-

Who’s the father?

Are you sure?

Use Multiple Probes

Eric S. Lander

An Meeker-O'Connell