clonación cell stem
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Clonación cell stem
http://www.abc.es/salud/noticias/celulas-piel-humanas-reprogramadas-convierten-14925.html
http://estaticos.elperiodico.com/resources/pdf/1/0/1368652108701.pdf
Somatic cell nuclear transfer
Align spindle and enucleatión pipette by rotating oocyte
Hold oocyte tightly make sure alignment by gently pushing the spindle with pipette tip laser assisted zona drilling
Gently insert pipette and aspirate spindles with….
small amount of cytoplast, as much as possible….
donor cell handling
Briefly soak donor cell into sev extract
donor cell pickup from sev extract
position spindle free oocyte
laser assisted zona drilling
injection donor cell into perivitelline aspace (NT)
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