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Chapter 6:CELLULAR RESPIRATION

3. The Citric Acid Cycle

2. Glycolysis

4. Oxidative Phosphorylation

1. Overview of Respiration

1. Overview of Respiration

What is Cellular Respiration?It is the process by which organisms use energy from “food” (e.g., glucose, fatty acids) to fuel the endergonic synthesis of ATP.

• requires (O2), occurs in most organisms (plants, too!)• provides a supply of usable energy for cells (ATP)

C6H12O6 CO26 H2O ATPs

Glucose Oxygen gas Carbon dioxide

6

Water Energy

O2 6+ + +

C6H12O6 6 O2 6 CO2 6 H2O

Loss of hydrogen atoms (oxidation)

Gain of hydrogen atoms (reduction)

Energy

(ATP)Glucose

+ + +

CellularRespiration andthe Human Body• respiratory system

acquires O2,removes CO2

• digestive system provides glucoseand fats as a sourceof energy

**cellular respiration takes place in mitochondria**

Respiration occurs in 3 stages:

1) Glycolysis

2) Citric Acid Cycle

• initial break down ofglucose

• completes the breakdown of glucose

• electron transport togenerate H+ gradient

3) Oxidative Phosphorylation

• chemiosmosis to produce ATP!

The Stages of Cellular RespirationNADH

NADH FADH2

GLYCOLYSISGlucose Pyruvate CITRIC ACID

CYCLE

OXIDATIVEPHOSPHORYLATION (Electron Transport and Chemiosmosis)

Substrate-level phosphorylation

Oxidative phosphorylation

Mitochondrion

and

High-energy electrons

carried by NADH

ATPATPATP

CO2 CO2

Cytoplasm

Substrate-level phosphorylation

1 2 3

(2) (2) (34!)

2. Glycolysis

Glucose

NAD++

22 ADP

NADH2

P2

2

ATP2 +

H+

2 Pyruvate

Glycolysis

+

ADP

ATP

Substrate

Enzyme

Product

Enzyme

P

P

P

A series of chemical reactions to break down glucose into two 3-carbon pyruvate molecules:

• energy-rich e- captured by e- carrier NADH

• occurs in cytoplasm, does NOT require O2

• yields a net of 2 ATP

2 ATP 2 NADH 2 pyruvate

substrate-levelphosphorylation

FermentationGlucose

NADH

NAD+

2

2 2 ADPP

ATP2

NADH2

NAD+2

2 ADPP

ATP2

2 Pyruvate

2 Lactate

GLY

CO

LYSI

S

GLY

CO

LYSI

S

Lactic acid fermentation

NADH

NAD+

2

2

NADH2

NAD+2

2 Pyruvate

2 Ethanol

Alcohol fermentation

Glucose

CO22released

+ 2 + 2

animals yeast

Instead of moving on to the Citric Acid Cycle, pyruvate will undergo fermentationwhen there’s not enough O2:

• fermentation productsinclude lactic acid (animals), ethanol (yeast)

• only 2 ATP per glucose(from glycolysis)

Fermentation “recycles” NAD+ from NADH so

glycolysis can continue

3. The Citric Acid Cycle

The Citric Acid Cycle occurs in

the “Matrix”• yield from 2

pyruvate (i.e., 1 glucose):

2 ATP

8 NADH

2 FADH2

What is the Citric Acid Cycle?

Where does it occur?

• series of chemical reactions that finish the break down of glucose• 3-C pyruvate from glycolysis broken down to 3 CO2

• in the mitochondrial matrix• pyruvate from cytoplasm gets transferred across inner

mitochondrial membrane into the matrix

Where does the released energy end up?• as high energy electrons (e-) in the electron carriers

NADH & FADH2 (plus heat)• e- passed on to the “electron transport chain” (ETC)

CoACoA

CO2

NAD+

NADHFAD

FADH2

ATP P

CITRIC ACID CYCLE

ADP +

3

3+

3 H+

Acetyl CoA

2

The Citric Acid Cyclepyruvate + CO2

4. Oxidative Phosphorylation

Overview of Oxidative Phosphorylation

“Oxidative Phosphorylation” refers to the additionof a 3rd phosphate to make ATP (“phosphorylation”)by a process that depends on oxidation-reductionreactions (“oxidative”), and occurs in 2 stages:

A) electron transport• e- from NADH and FADH2 are passed along the electron

transport chain (ETC) via oxidation-reduction reactions

• convert energy from e- into energy stored in H+ gradient

B) chemiosmosis• energy from H+ gradient used to make ATP

OH H O 2H

Reduction

(carries 2 electrons)

NAD+ 2H

2H+ 2e-

NADH H+

Oxidation

+

+

+

+

Oxidation & ReductionOXIDATION – loss of e- (usually with a hydrogen atom)

REDUCTION – gain of e- (usually with a hydrogen atom)

(loss of H)

(gain of H)

e- Transport generates H+ Gradient+ 2 e-

• energy from e- used to pump H+ ions (fr. lo hi conc)

Electron TransportHigh energy electrons (e-) from NADH, FADH2transferred to e- transport chain (ETC)

Energy released through e- transport used to create H+ gradient (necessary to make ATP)

• ETC is a series of proteins in the inner mitochondrial membrane through which e- are passed

• H+ pumped across inner mitochondrial membrane out of the matrix into the intermembrane space

Oxygen (O2) is essential for electron transport!• O2 is the final electron acceptor at the end of the ETC

**no O2 = no e- transfer = no ATP production = death!**

Chemiosmosis used to make ATPchemiosmosis = diffusion of H+

• ATP synthase uses energy of H+ flow to make ATP

Chemiosmosis

Energy derived from the flow of H+ is used to synthesize ATP (from ADP & Pi)

• H+ flows “down” concentration gradient through ATPsynthase in the inner mitochondrial membrane

Yields up to 34 ATP per glucose molecule!

“The flow of a substance (H+) from high to low concentration”

• in addition to 2 ATP in glycolysis, 2 ATP in Krebs cycle

• ATP synthase = enzyme complex that catalyzes:

ADP + Pi ATPH+ flow

Intermembrane space

Inner mitochondrial

membrane

Mitochondrial matrix

Protein complex

Electron flow

Electron carrier

NADH NAD+

FADH2 FAD

H2OATPADP

ATP synthase

H+ H+ H+H+

H+ H+

H+H+

H+

H+

H+

H+

H+

H+

+ P

1 O2

Electron Transport Chain Chemiosmosis

.

OXIDATIVE PHOSPHORYLATION

+ 2

Summary of Oxidative Phosphorylation

Respiration is simply a Series of Energy Conversions!

I. Energy stored in sugars and fats is converted to energy temporarily stored in electron carriers(i.e., the e– of H in NADH & FADH2)• via Glycolysis & the Krebs Cycle

II. Energy stored in NADH & FADH2 is converted to energy temporarily stored in an H+ gradient• via electron transport

III. Energy stored in an H+ gradient is converted to energy stored in molecules of ATP• via chemiosmosis (which fuels ATP synthesis)

Key Terms for Chapter 6

• ATP synthase

• electron carriers (NADH, FADH2)

• electron transport chain (ETC)

• glycolysis, fermentation

• oxidative phosphorylation, chemiosmosis

• Citric Acid Cycle

• oxidation vs reduction