castaway: precast sequencing system features and benefits
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CastAway: Precast Sequencing System
Features and Benefits
CastAway:: It’s Easy Easy1. Remove gel from bag.
2. Place gel in sequencing device
3. Slide gel into dryer
4. Analyze results
CastAway: It’s Fast
CastAway: It’s Safe
Unpolymerized acrylamide is a neurotoxin and therefore hazardous to work with, since CastAway gels are provided pre-polymerized, exposure to toxic acrylamide is minimized.
CastAway: It’s Consistent Approximately 15-25% of manually cast
polyacrylamide sequencing gels are discarded due to:
-leaks-bubbles -poor polymerization
CastAway gels are insured by Stratagene’s quality control for perfect gels, every time.
……And Even Gel Drying is And Even Gel Drying is EasierEasier
It is no longer necessary to transfer your gel to filter paper– The gel is fixed to one of the glass plates and
dried directly on the plate
No noisy vacuum pumps that are difficult to maintain– The CastAway dryer uses forced air to dry your
gels in only 30 minutes
CastAwayCastAway System System
ComponentsComponents
Complete CastAway SystemComplete CastAway System100/120v #401098100/120v #401098 230v #401099 230v #401099
Sequencing Device Gel Dryer Fixing Trays (2) Radiation Storage Container Shark-Tooth Comb Set (3 each 20-, 32-, 48-
well combs) High-Voltage Leads Gasket Lubricant
CastAway Sequencing Device
Catalog #401070 Sequencing Device
Dimensions: 48.5 cm H x 30 cm W x 17 cm D Weight 3 kg
Shark-Tooth Comb Set (3 each 20-, 32-and 48-well combs)
High-Voltage Leads Gasket Lubricant
CastAway Cassettes and Kodak BioMax MR Film
CastAway Autoradiography Cassettes #401116 -Compact and lightweight -18 x 43 cm, perfectly fit CastAway gels.
Kodak BioMax MR Film #401117 -The preferred film for high resolution sequencing, sized to fit CastAway gels and cassettes.
CastAway Gel-Loading Syringes
Single-Channel Syringes #401086 -0.2mm diameter needle fits easily into the wells of a CastAway gel
8-Channel Syringes #401087 -For use
with Shark-Tooth comb for 8-channel pipettors
CastAway Silver Staining Kit Novel Enhancing Reagent
provides higher contrast silver staining with lower background than conventional methods
Single use packaging for staining CastAway gels
Easy DNA recovery: bands can be removed from the gel and used directly for reamplification without delays for purifying the gel fragments or film exposure.
Comparison of PAGE data from conventional silver staining method (A) and the CastAway silver staining kit (B)
75mer
50mer
35mer
20mer
1 2 3 4 1 2 3 4
(A) (B)
CastAway Rehydration Kit and Components
Rehydration Kit #401076-10X Rehydration Buffer-Gel Blades (5)-Scraping Trays (2)
10X Rehydration Buffer #401077 Gel Blades (5) #401078 Scraping Trays (2) #401079
CastAway 6% Precast Gels
6% Polyacrylamide Gels #401090-6% polyacrylamide, 7M urea, 1x TBE
6% Nondenaturing Gels #401096 -6% polyacrylamide, 1x TBE
6% Gels w/Preformed Wells #401094 -6% polyacrylamide, 7M urea, 1x TBE -32 preformed wells, compatible with
8-channel pipettors
CastAway 4.5% Precast Gels
4.5% Polyacrylamide Gels #401091-4.5% polyacrylamide, 7M urea, 1x TBE
4.5% Gels w/ Preformed Wells #401092-4.5% polyacrylamide, 7M urea, 1x TBE-32 preformed wells, compatible with 8-channel pipettors
CastAway Long RangerTM Gels
5.5% Long Ranger Gels #401097-5.5% Long Ranger gel, 8.3M urea,
1x TBE-Up to 30% longer reads compared
to standard gel matrix
CastAway 12% Precast Gels
12% Polyacrylamide Gels #401093 -12% polyacrylamide, 7M urea, 1x TBE
-32 preformed wells, compatible with 8-channel pipettors
CastAway 8% Precast Gels
8% Polyacrylamide Gels #401088 -8% polyacrylamide, 7M urea, 1x TBE -32 preformed wells, compatible with 8-channel pipettors
CastAway Gels for the CastAway Gels for the LI-COR SystemLI-COR System
The first precast gels for automated sequencing and genetic analysis
25cm gels for the LI-COR IR2 system Run up to 32 sequencing samples or 128
genotyping lanes Gels were developed specifically for use on the
LI-COR system and will not fit the CastAway sequencing device
Automated sequencing and background Automated sequencing and background fluorescencefluorescence
It is unlikely that these precast gels could be used with visible fluorescence automated DNA sequencers such as the ABI PRISMR 377 since the system sensitivity would be lower due to significant emission of background fluorescence by the soda-lime plates.
With the LI-COR system, by using infrared radiation, the background fluorescence due to the soda lime glass of the precast gel is greatly reduced.
Applications:
It’s Not Just For Sequencing
Select the Right Gel for Your ApplicationSelect the Right Gel for Your Application
Application
6% gel, 7Murea w/ orw/o pre-formed wells
6% non-denaturinggel
4.5% gel, 7Murea w/ orw/o pre-formed wells
8% gel, 7Murea w/pre-formed wells
12% gel, 7Murea w/ pre-formed wells
5.5% LongRanger gel,8.3M urea
Sequencing Silver staining DifferentialDisplay/RAP PCR
DNAFingerprinting AFLP SSCP RNAseProtection Assay DNAFootprinting PrimerExtensions OligonucleotideAnalysis MicrosatelliteDNA Analysis
3535S SequenceS Sequence Silver Stained SequenceSilver Stained Sequence
Sequencing
Differential Display/RAP PCR
Figure 1 Figure 2
Figure 3
Figure 1: Differentially displayed products on 6% CastAway gel.
Figure 2: Products to be cut out & reamplified are outlined on the autorad
Figure 3: Reamplified products
Multiplexed PCR ReactionMultiplexed PCR Reaction
Multiplexed PCR reactions were run on a CastAway 8% gel and analyzed using Hitachi’s FMBioII Scanner. The pattern of banding, corresponding to family specific CDR3 length polymorphisms allows comparison of T cell receptor utilization by peripheral blood lymphocytes with that of breast tumor-infiltrating lymphocytes.
RAP PCR Kit control reactions performed
with HepG2 mRNA and C2/C3 primer sets
4.5% CastAway gel w/ 32 pre-formed wells
NEWNEW SmartPower™ 4000 SmartPower™ 4000 Power SupplyPower Supply Catalog # 400690 100/120 Volt Catalog # 400691 230 Volt CE Marked Catalog # 400692 230 Volt non-CE Marked
SmartPower™ 4000 FeaturesSmartPower™ 4000 Features
All-purpose: broad voltage and amperage control ranges make this a multifunctional power supply for both horizontal and sequencing gels- unique feature!
Temperature Control: built-in StrataTherm® temperature controller
Multiple power outputs: Four sets of output jacks enable multiple gels to be run simultaneously
SmartPower™ 4000 FeaturesSmartPower™ 4000 Features
Independent Power Output Monitoring: Current and power can be monitored on each output jack independently- unique feature!
Programmable: the user can create and save commonly used power parameters as individual programs
Timer Control: built-in timer for automatic program start or stop
SmartPower 4000
BioRad PowerPac 3000
E-C Apparatus EC4000P
Pharmacia EPS 3500 XL
Gibco BRL Model 40001P
Voltage 25-4000V 25-3000V 4000V 3500V 4000VCurrent 1-450mA 1-400mA 300mA 150mA 300mAPower 1-450W 1-400W 300W 100W 300WTemp control Yes Yes No No NoTimer Yes Yes Yes Yes YesPower outputs 4 4 4 2 4Programmable Yes Yes Yes Yes YesOutput monitoring Independent
non-Independent
non-Independent
non-Independent
non-Independent
Price 2,495.00$ 1,795.00$ 2,250.00$ 2,295.00$ 2,495.00$
Joule BoxJoule BoxTMTM
Mini Gel Electrophoresis UnitMini Gel Electrophoresis Unit Horizontal electrophoresis device with built-in, mini
power supply Versatile - for both agarose and polyacrylamide gels Gel casting set for pouring up to
6 gels simultaneously CE Marked/UL approved 4 comb sizes for various
loading volumes
New Joule Box AccessoriesNew Joule Box Accessories 10cm Casting Set
Run one 10cm gel or vary comb placement for several shorter runs
Shaded comb insertion areas and gel migration distance lines for easier sample loading and quick determination of run distance
Preparative Comb Set Several comb formats, including large
wells for loading preparative samples
Joule BoxJoule BoxTMTM Vs. The Competition Vs. The Competition
The Joule Box apparatus contains an anti-fogging reagent rather than ventilation slits to prevent condensation inside lid. This technology produces a system which is much safer to run & allows it to be CE Marked.
Joule Box casting trays feature shaded comb insertion areas and migration distance index lines to easily determine gel running distance at a glance.
EliminatorEliminatorTMTM
Dye Removal SystemDye Removal System Up to 99% removal of ethidium
bromide dye from buffers Buffers can then be disposed of without
environmental risk Each Eliminator sleeve can purify 20
liters of liquid No vacuum manifold or pre-
equilibration needed, simply pour buffer into sleeve and allow to drain by gravity flow
Researchers need to be educated– Although there are no environmental standards
for EtBr waste disposal, Ethidium Bromide is a MUTAGEN, and should be disposed of properly - with the ELIMINATOR Dye Removal System!!
Focus on selling to Environmental Health and Safety Departments at all major institutions
Make the Eliminator the standard for Ethidium Bromide waste disposal
LC50 Index = 0.00 (cut off level = 500) - based on testing by Del Mar Analytical, Feb. 1999
EliminatorEliminatorTMTM vs. The vs. The CompetitionCompetition
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