bioinformatik i Übungen · a. zinc fingers b. helix-turn-helix c. leucine zipper d....
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BIOINFORMATIK I ÜBUNGEN
http://icbi.at/bioinf
Regulation of transcription
TF classification by DNA‐binding
A. Zinc fingers B. Helix-turn-helix
C. Leucine zipper D. Helix-loop-helix
• hetero dimerization
• homo dimerization
Transcription factor dimerizationLeucine zippers
Protein 3D‐structure
Example: PPARg::RXRa+Rosi, 9‐cis RA, NCOA2 (3dzy)
• PDB• JMol• Cn3D• POLYVIEW‐3D• Proteopedia
PDB identification code
Recombinant DNA technology
• Protein synthesis
• In vitro expression
• Libraries
• Gene transfer in animals and plants
Recombinant DNA technology
• Polymerases
• Nucleases
• restriction enzymes
Recombinant DNA technology
Recombinant DNA technology
• Ligases
Cloning
Cloning vector (pBR322)
• Plasmid
• Ampicillin resistance
• Tetracycline resistance
• Polylinker
• Origin of replication (ori)
Selection by antibiotics
Selection by X‐gal
Exercise 3
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