assessment of genetic hotspots for phytophthora - agro.au.dk · development of late blight...

Development of Late Blight (Phytophthora infestans) Resistant Potato Breeding Material for Organic Farming

Assessment of genetic hotspots for Phytophthora resistance and their use as molecular markers

in potato breeding

BLE-Projekt 2810OE071 (LfL), 2810OE121 (JKI), 2810OE122 (IPK)

K. Sieber, G. Forster, A. Berger, A. Schwarzfischer und A. Kellermann

Aims and objectives

Fotos: JKI Dr. Karen Sieber 2

Field Trial: 150 cultivars and breeding clones have been grown and

examined under organic farming conditions.

Molecular Analyses: Examination of resistance hotspots and evaluation of genetic

markers for resistance breeding

Potato Breeding: We aim at combining late blight resistance with quality traits. For the selection of breeding material we use a participative

approach on organic farms

Fotos: JKI Dr. Karen Sieber 3

Field Trial: 150 cultivars and breeding clones have been grown and

examined under organic farming conditions.

Molecular Analyses: Examination of resistance hotspots and evaluation of genetic

markers for resistance breeding

Potato Breeding: We aim at combining late blight resistance with quality traits. For the selection of breeding material we use a participative

approach on organic farms

Aims and objectives

Field trial

Fotos: JKI

Varieties for field trials 2014 Total: 153 cultivars • JKI (28) • LfL (26) • IPK (10) • German potato breeders (58) • others (26) • reference (5)

Dr. Karen Sieber 4

Fotos: JKI

GL: Groß Lüsewitz UE: Uelzen ND: Neuburg a.d. Donau) FS: Freising LL: Landsberg am Lech

natural infection / organic trial field

artificial infection

pest control / maturity test

GL

FS LL

ND

UE

Dr. Karen Sieber 5

Field trial

Fotos: JKI

year number of varieties

environments trials per environment

individuals per plot

2012 117 3 (LL, ND, UE)

2 10

2013 149 1 (FS)+ 3 (ND, LL, UE)

2 10

2014 153 1 (FS) + 3 (LL, ND, UE)

2 10

Parameter Sprouting time development growth form maturity late blight yield

tuber shape

tubers per plant

starch content tuber size

quality frying quality

Dr. Karen Sieber 6

Field trial

Fotos: JKI

Trial field in Schrobenhausen 2012

Dr. Karen Sieber 9

Field trial

Fotos: JKI Dr. Karen Sieber 10 Fotos: JKI

UE: Uelzen ND: Neuburg a.d. Donau) LL: Landsberg am Lech

other

EU_13_A2

LL

ND

UE

Field trial: Phytophthora Monitoring

Fotos: JKI Dr. Karen Sieber 11

days to maturity

days to maturity

LL 2014 Walleshausen LK Landsberg

days to maturity

Δ

rAU

DPC

Δ

rAU

DPC

UE 2014 Natendorf LK Uelzen

Corrected Late Blight Resistance and Maturity for Black’s Differential Set

Field trial

Fotos: JKI Dr. Karen Sieber 12

Field trial

GD 5%

GD 5%

Saka6

Kuras

Hubal

TuebingerCirce

Biogold

Vitabella Bionica

Carolus

Toluca Axona

SarpoMira

-0,4

-0,3

-0,2

-0,1

0,0

0,1

0,2

80 90 100 110 120 130 140

∆rAU

DPC

maturity [days]

Late Blight Resitance and Maturity 2012-2014

JKI Population Anuschka Princess Lolita Jelly Ditta

Aims and objectives

Fotos: JKI Dr. Karen Sieber 13

Field Trial: 150 cultivars and breeding clones have been grown and

examined under organic farming conditions.

Molecular Analyses: Examination of resistance hotspots and evaluation of genetic

markers for resistance breeding

Potato Breeding: We aim at combining late blight resistance with quality traits. For the selection of breeding material we use a participative

approach on organic farms

Potato breeding

Fotos: JKI Dr. Karen Sieber 14

Seedling potatoes in Landsberg 2014

Potato breeding

Fotos: JKI Dr. Karen Sieber 15

Participative selection of breeding clones in Landsberg 2014

Potato breeding

Fotos: JKI Dr. Karen Sieber 16

Susceptible plants will be sorted out

Potato breeding

Fotos: JKI Dr. Karen Sieber 17

Harvest of preselected clones in Landsberg n 2014

Potato breeding

Fotos: JKI Dr. Karen Sieber 18

num

ber o

f clo

nes

trial set 2012-2014

Comparison of Late Blight Resistance Values

breeding clones LL 2014 Resistance values (1=resistant, 9=susceptible)

Potato breeding: resistance vs yield

Fotos: JKI Dr. Karen Sieber 19

Aims and objectives

Fotos: JKI Dr. Karen Sieber 20

Field Trial: 150 cultivars and breeding clones have been grown and

examined under organic farming conditions.

Molecular Analyses: Examination of resistance hotspots and evaluation of genetic

markers for potato breeding

Potato Breeding: We aim at combining late blight resistance with quality traits. For the selection of breeding material we use a participative

approach on organic farms

Molecular Analyses: Wild Resistance Donors

Fotos: JKI 21

S. demissum

S. stoloniferum

S. pinnatisectum

S. bulbocastanum

S. acaule S. phureja

Fotos: JKI 22

STAos2 45/XI

Molecular Analyses: Resistance Loci

Fotos: JKI Dr. Karen Sieber 24

S. demissum R1

IV

Molecular Analyses: Markers for S. demissum

R3

XI • We applied regions from R1

and R3b as molecular markers.

• Sequences are identical for all investigated cultivars.

• R1 appears in ca. 12% of all trial samples

• R3b appears in ca. 35% of all trial samples

Products: R1, R3a, R3b References: Huang et al. 2005, Rietman 2011, Lokossou et al. 2009; Kim et al. 2012

Fotos: JKI Dr. Karen Sieber 25

S. bulbocastanum

Molecular Analyses: Markers for S. bulbocastanum

• Markers Blb3 and Abpt were established for the blb3 region

• Blb1-820 was used for the blb1 region

• 9% of all samples were positive at the blb3 locus

• 1 sample was positive at the blb1 locus.

Products: − Blb3, Abpt, − RB-629, Blb1-820, RB-226,

Sto1 References: − Lokossou et al. 2009; Kim et

al. 2012; − Fadina et al. 2009; Zhu et al.

2012

blb3

IV

blb1

VIII

Fotos: JKI Dr. Karen Sieber 26

Molecular Analyses: Markers for S. bulbocastanum

GD 5%

GD 5%

04.5214.03 blb3

04.5214.04 blb3 + blb1

05.5049.10 blb3

07.1018.05 blb3 93.6984.07 blb3

94.7082.15 blb3

96.7395.03 blb3

97.7550.02 blb3 98.7826.02 abpt

-0,40

-0,30

-0,20

-0,10

0,00

0,10

0,20

80 90 100 110 120 130 140

∆rAU

DPC

Maturity [days]

Late Blight Resistance Values

Anuschka Princess Lolita Jelly Ditta JKI

Molecular Analyses: Wild Resistance Donors

Fotos: JKI 27

S. bulbocastanum

S. stoloniferum

??

S. andigena

??

Fotos: JKI Dr. Karen Sieber 28

Molecular Analyses

n=38 n=9 n=56

n=9

n=14

-0,15

-0,10

-0,05

0,00

0,05

0,10

0,15

ohne Marker R1 R3b R1 + R3b blb3 / abptΔrAU

DPC

Late Blight Resistance Values

Perspectives

Fotos: JKI Dr. Karen Sieber 29

• S. venturii • S. phureja

IX • Continue breeding efforts and selection of

breeding clones

• Analyse of SNP results

• Find molecular markers for other Solanum species that were used as resistance donors

• Develop markers that allow to distinguish between resistance genes and their inactive homologues

Thank You!

Fotos: JKI Dr. Karen Sieber 30

Georg Forster

Adi Kellermann

Andrea Schwarzfischer

Andi Berger

Project Team

Fotos: JKI Dr. Karen Sieber 31