application of metagenomics data pool in structured ...bdellovibrio sp - obligate aerobic bacteria...

Application of Metagenomics Data Pool

in Structured Problem Solving

Kalliopi Chalkou

Group Food Safety / SQ Manager

Zoltan Syposs

Group QSE Director

A Bottled Water Industry Example

Our portfolio requires a more sensitive approach to

Food Safety

Only Natural ingredientsNo preservatives

2000

Carbonated Sensitive BeveragesNo Carbonation

Preservatives Artificial ingredients

Natural ingredients No preservatives

2010 2020

80%90% 60%

We started receiving repeated and sporadic consumer

complaints for off-note in one of our NMW

Off -note characterized as

Sulfuric

…never been able to detect

during production

Bottled Water Food Safety Incidents 2015 – 2018

Total incidents EU incidents

Thousands of Samples

indicated full compliance

…or not?

Collaboration & Partnerships with Universities

Some of our Partnerships on:

❖ Methodology development

❖ Structured problem solving

❖ Youth development/Education

❖ Research topics

❖ Trainee programs

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Fixed vs.

Growth

Mindset

Go beyond the Extra

Mile…

Scrutinize the facts

..The need to see the “small” picture

Changing our approach

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• Samples from all process steps + off-note complaints

• Community DNA extraction

• Next Generation sequencing (NGS) – High Throughput

• Phylogenetic assignment (OTUs), estimation of α, β diversity

Wells

Collector

Sand Filter

Sporadic off note

Microbiological sampling pointSensory analysis sampling point

Buffer Tanks

FillerFilter units

We mapped the process again…

We built a new NMW specific database

Wells Buffer/ Production tanks

Biofilm indication in the tanks:

Species with presence < 1% accumulate and overgrow in the

buffer and production tanks

Biodiversity Decrease / Species dominance increase

Abundant species in samples with off note

Abundant species in off note samples low presence in previous process steps

Species detected in off note samples

Perlucidibaca sp and Polaromonas sp - Reducing nitrate and produce indole, heterocyclic organic compound

Limnobacter sp - Representatives that exceeded 70% samples are heterotrophic, aerobic thiosulfate oxidizing

Cytophagaceae - Commonly isolated from marine, freshwater, and terrestrial habitats

Bdellovibrio sp - Obligate aerobic bacteria that are considered bacterial predators and parasites. Biofilm colonizers

Methylotenera sp - Obligately methylamine-utilizing species aerobic, Gram-negative

Wells Finished Product

In presence of carbon from organic compounds, bacteria metabolize Selenium to dymethylediselenide (DMDS), dymethyleselenide (DMS) and similar compounds which could be detected in very low concentration by

The above mechanism is not valid

Root Cause Analysis

For PET

For RGB

❖ Apply Biofilm build up Prevention in filling Equipment

❖Try again…until the Root Cause fully understood

❖ Build structured data

❖ Listen to Data

❖ “Scrutinize the Facts”

Lessons Learned

Bottled Water Quality/Food Safety Incidents 2015 – 2018

Thank you

No off-note complaints in the past >12 months

Thank You!