anastasia gorbunova, ma (university of arizona) modality instructor: dr. justin crowley

Anastasia Gorbunova, MA (University of Arizona)Modality instructor: Dr. Justin CrowleyTraining Assistant: Corey Flynn

MNTP2009: Optical Imaging Modality

“Destructive interaction or interference among transient stimuli that are closely coupled in space or time”

R. Fox. (1998). In Handbook of Sensory Physiology. VIII. Perception.

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Forster & Davis (1984) J Exper Psych: Learning, Memory & Language

Optical imaging of intrinsic signal- wide spatial field- temporal resolutionslow hemodynamic dependentfaster light scattering properties of tissue

Single unit recordings - best temporal resolution Two-photon Calcium imaging

- distinct neurons, fine time course

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3.

2.

4.

Time

Time Time

Time

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Time

Time

• Exposed visual cortex of a ferret (P80-130)

• Illumination by ~700 nm light

• Visual stimulation (4 degree bars)

• CCD camera (100 ms data frames)

• Computer digitization

8000 ms

Courtesy of David E. Whitney

Time

Stimulus 1

Stimulus 2

Blank stimulus 8.1 s

0.1 s

0.1 sInter-Stimulus Blank 3 s

ISB 3 s

ISB 3 s

8 s

8 s

1 Block

- =

100 ms 100 ms 100 ms

8 s 8 s 8 s

100 ms

8 s

100 ms

8 s

100 ms

8 s 1 frame: 500 ms

• Exposed visual cortex of a ferret (P80-130)

• Carbon fiber electrode• 20kHz signal digitization

H-B

H-B

V-B

H-V

V-H B H HV V

SQ

P

SQ

P

SN

P

SN

P B

V-H

H-V

V-B

H, horizontal grating; B, blank; V, vertical grating; SQP, square-wave plaid; SNP, sine-wave plaid

Oregon green BAPTA-1AM (labels neurons and astrocytes)

Sulphorhodamine-101 (labels astrocytes)

Microscope: Zeiss LSM 510 Meta NLO with MIRA900 Ultrafast Ti:Sapphire laser

20x NA1 objective

3 cells: ΔF/F for 700 frames normalized over 10 blocks

It is possible to study masking in animal models using simple visual stimuli

Very brief presentation of static gratings produces sufficient activation detectable by optical imaging of intrinsic signal

Orthogonal mask disrupts that activation

Dr. Justin CrowleyCorey FlynnDavid WhitneySruthi ChintakuntaAlberto Vazquez

Dr. Seong-Gi KimDr. Bill EddyTomika CohenRebecca Clark

Thank you!