تجميعات صور أبحاث هستوباثولوجي

Histopathology photoscollections

Histological characterization of wound healing of flank verses midline ovariohysterectomy in different age groups of cats

Plate 1. Photograph of abdominal skin with acute inflammation.

Plate 2. Photograph of abdominal skin wound with (arrow) chronic inflammation.

Plate 3. Photograph of abdominal skin wound inflammatory cells.

Plate 4. Photograph of abdominal skin (arrow) with (arrow) granulation.

Plate 5. Photograph of abdominal skin wound (arrow) neovascularization.

Plate 6. Photograph of abdominal skin wound with (arrow) collagen fibres.

Macroscopic and microscopic changes in the wound after intradermal

closure using buried knot and pulley knot-free patterns following ovariectomy in cats

Figure 3. Cross section of the skin from cat with buried knot anchor day 7 post-operative; showing the presence of moderate epidermal invagination (Grade 2; black arrow) and moderate epidermal thickening (Grade 2, T). E (epidermis), D (dermis). Hematoxylin-eosin stain.

Figure 4. Cross section of the skin of cat with buried knot day 7 post-operative; showing the presence of moderate wound gap (Grade 2; black arrow). E (epidermis), D (dermis). Hematoxylin-eosin stain.

Figure 5. Cross section of the skin of cat with pulley knot-free day 21 post-operative in (a) normal histopathology view and (b) using polarized lens; showing the pres-ence of abundant neutrophils (inflammatory cells) around the suture material (Grade 3). D (dermis), I (inflammatory cells), V (suture material). Hematoxylin-eosin stain.

Rapid reperitonealization and wound

healing in a preclinical model of abdominal trauma repair with a

composite meshFig. 1. Gross morphology and H&E staining study intervals. a) Animal 66734 Left Implant. Glistening of the mesh surface consistent with early mesothelial cell attachment. g) Animal 66734 Left Implant. Mesothelial cell line the outer surface of the mesh (arrows). b) Animal 66737 Left Implant. Glistening of the mesh surface is consistent with early mesothelial cell attachment. h) Animal 66737 Left Implant. Few fibroblasts (black arrows) are located between the filaments and the film and between filaments. The outer surface is covered by mesothelium (white arrows). Processing artifacts are present including separation of the mesh from the underlying tissue and segmental loss of mesothelial covering. c) Animal 66729 Left Implant. Glistening of the mesh surface is consistent with early mesothelial cell attachment and formation of a neoperitoneum. i) Animal 66729 Left Implant. More fibroblasts are within the mesh penetrating through breaks in the films and the covering mesothelial surface (neoperitoneum) is more mature than in the previous study intervals processing artifacts are present including separation of the mesh from the underlying tissues and segmental loss of the mesothelial covering. d) Animal 66731 Left Implant. Glistening of the mesh surface is consistent with early mesothelial cell attachment and formation of a neoperitoneum. j) Animal 66731 Left Implant. More breaks in the films were present with fibroblasts streaming into and through the mesh. The outer surface has well established mesothelial layer (neoperitoneum) of the mesh. e) Animal 66732 Left Implant. The mesh is covered by tissue. k) Animal 66732 Left Implant. The mesh is completely incorporated and integrated with the underlying tissues. The film components of the mesh are fragmented. f) Animal 66733 Left Implant. The mesh is covered by tissue. l) Animal 66733 Left Implant. The mesh is completely incorporated and integrated with the underlying tissue. The film components of the mesh are fragments.

Cutaneous reepithelialization and wound contraction afterskin biopsies in rabbits: a mathematical model for healing and

remodelling index

Fig. 3. After 2 days (A and B, Masson trichrome, modifi ed by Goldner), the wound is still open and lined by a granulation tissue covered with fi brin, which appears red to black (yellow arrows). At the bottom of the wound, the thickness of the residual dermis is very small (in green),subcutaneous muscles are very close (in red-orange). Residual hematoma is still visible (in yellow, just above the muscles, black arrow). Wound contraction is mainly superfi cial.

Cutaneous reepithelialization and wound contraction afterskin biopsies in rabbits: a mathematical model for healing and

remodelling index

Fig. 4. After 9 days, reepithelialization is complete (A and B, Mallory trichrome staining). The wound is closed with a thick new epidermis. The scar tissue at the centre of wound is a very denseand unorganized matrix, stained in blue. The mature matrix, stained in red, is converging to the remodelling area. Colorimetric differentiation is neat with Mallory trichrome.

Evaluation of the Tissue Reaction of Five Different Suture Materials in Rabbit Palatal Mucosa

Figure 2. Illustration of Zone 1 (Z1) and Zone 2 (Z2)

Evaluation of the Tissue Reaction of Five Different Suture Materials in Rabbit

Palatal Mucosa

Figure 3. Five suture specimen at 2, 4, 8 days illustrating areas with inflammatory infiltrate in the connective tissue. A, Silk; B, Catgut; C, Polypropylene; D, Dacron; E, P 910 (Hematoxylin and eosin stain; bar=0.1 mm).

Histological Evaluation of Incision Healing Response Made by Metallic Scalpel on Rabbits Skin: Preliminary Study

Figure 1. Light micrographs of rabbit's skin portions exhibiting the incisions made with the scalpel (a, e, i, m, q, u, y), At 12 h, 1day, 3days, 7days, 14days, 21days and 28days of experiment, sections stained with H&E. x 20. IC: inflammatory cells, TR: tissue remnants, BC: blood clot, HR: hyperactivity of tissue, RE: re-epithelisation, CF: collagen fibres, GT: granulation tissue, TP: thin epithelium, FV: fibro vascular connective tissue, FTE: full thickness epithelium,EH: epithelial hyperplasia, BV: blood vessels, TE: thin epithelium, ST: scar tissue.

Histopathologic comparison of new suture pattern with continuous mattress suture

New suture materials for midline laparotomyclosure: an

experimental study

Figure 3 Light microscopy images. Panoramic view of the control linea alba at 3 weeks (A) and 6 months (F) and in the midline closureperformed using the different sutures at 3 weeks (B-E) and 6 months (G-J). (Masson’s trichrome stain, 100x). s: suture, LA: linea alba, Re: rectusabdominis muscles, Ss: subcutaneous side, Ps: peritoneal side, PP: Surgipro™, PUe: Assuplus®, PDX: Assufil® and PDXb: Filbloc®.

New suture materials for midline laparotomyclosure: an experimental study

Figure 5 Macrophage response to suture materials. Immunodetection of RAM-11 rabbit macrophages at 3 weeks (A-D) and 6 months (E-H) post intervention (Top panel, 200x). s: suture; *: non degraded remains of PDX. Results expressed as the mean ± SEM of RAM-11 positive macrophage percentages. Mann-Whitney U Test. *, p < 0.05; ***, p < 0.001. PP: Surgipro™, PUe: Assuplus®, PDX: Assufil® and PDXb: Filbloc®.

Histopathology assessment of incisional wound-healing behaviour of peritoneum-sutured and not-sutured techniques following laparotomy and

omentopexy inWest African dwarf (WAD) goats

Plate 1 Section of muscle sutured with peritoneum (GPA) on psd 3. Noteintense eosinophilia of necrotic muscle fibres (N), mild polymorphonuclear/mononuclear leucocyte infilteration and intense myoglobin deposition (P),with mild collagen deposition at the edge (C). H&E ×100

Plate 2 Section ofmuscle sutured with peritoneumand omentum (GP C)on psd 3, showing hyperaemia/haemorrage (H) and mild fibroplasia as

part of the acute inflammatory process. H&E ×100

Plate 3 Section of muscle sutured without peritoneum (GP B) on psd 3,showing segmental degeneration (M) and necrosis (N) without cellularinfiltration. H&E ×100

Plate 4 Section of muscle sutured with peritoneum (GP A) on psd 7,showing intense infiltration of phagocytic cells and proliferatingmyoblast(PM).Note the remaining necrotic muscle fibres (N). H&E stain ×100

Plate 5 Section of muscle sutured with peritoneum and omentum(GP C)on psd 7, showing predominantly myoblast and fibroblast followingalmost complete removal of necrotic tissue. H&E ×100

Plate 6 Section of muscle sutured without peritoneum (GP B) on psd 7,showing lysis of necrotic muscle (N) andwide area of necrosis (M).H&E ×100

Plate 7 Section of muscle sutured with peritoneum (GP A) on psd14,showing hyperaemic blood vessels (H) and fibrous connectivetissue/collagen deposition (arrow). H&E ×400

Plate 8 Section of muscle sutured with peritoneum (GP A) on psd 42,showing evidence of collagen deposition (C), myocyte proliferation andfew fibroblast (arrow). H&E ×100

Plate 9 Section from muscle sutured with peritoneum and omentum (GPC), on psd 42, showing myofibre formation (M) and thin connectivetissues between the muscle bundles (arrow). H&E ×400

Plate 10 Section frommuscle sutured without peritoneum(GP B) on psd42, showing muscle fibres (M) and extensive collagen deposition (C).H&E ×400

Evaluation of the abdominal wall cicatrization of rabbits exposed to nicotine and undergone abdominoplasty using

nylon thread or cyanoacrylate1FIGURE 3 - Photomicrograph of cicatrization area showingthe eosinophilic inflammatory infiltrate.

Histologic characterization of vaginal vs. abdominal surgicalwound healing in a rabbit model

Figure 1. (A) Photomicrograph of an abdominal skin wound at 4 days after wounding (hematoxylin & eosin stain, 20 magnification) with moderate to marked acute inflammation (score 2 out of 3) and a prominent inflammatory crust (arrow). (B) Higher (40) magnification of the specimen shown in A demonstrating the acute inflammatory exudate composed of neutrophils and an inflammatory crust overlying an immature (score 1 out of 3) granulation tissue (arrow).

Figure 3. Photomicrograph of an abdominal wound at 7 daysafter wounding (hematoxylin & eosin stain, 40 magnification)with marked chronic inflammation (score 3 out of 3).

Figure 7. Photomicrographs of vaginal (A) and abdominal (B) wounds at 7 days after wounding (hematoxylin & eosin stain, 20 magnification)with prominent neovascularization (score 3 out of 3) (arrows) and moderatechronic inflammation (score 2 out of 3).

Figure 9. Photomicrograph of an abdominal wound at 14 days after wounding (hematoxylin & eosin stain, 20 magnification)with almost complete reepithelialization (score of 2 out of 3).

SUTURE MATERIALS AND PATTERNS JACQUI NILES AND JOHN WILLIAMS

Polyglactin 910, day 14. There is minimal tissue reaction around the suture

Chromic catgut, day 28. There is continued active cellular tissue reaction

Polydioxanone, day 7. The area of tissue reaction is minimal

Polydioxanone, day 28. The suture is surrounded by a narrow mature zone of tissue reaction

HISTOLOGICAL STUDY OF EARLY EVENTS DURING WOUND HEALING IN RABBITS

Fig. (1): In wound show the epidermis (Ep) was thickened at its cut edges & inflammatory reaction (N) (400X, H&E)

Fig.(2): show the epidermis (Ep) was thickened at its cut edges& tissue necrosis and erosion (400X , H&E)

Fig.(3): observe that necrotic (Ne) on the surface was almost removed and the scab was forming, thickened (Th) edges of epidermis –keratinocytes migrated beneath the scab

(400X , H&E)

Fig.(4): The major marker of acute inflammation if those cells were present, they were found epithelial layers (400X , H&E)

Simvastatin Improves Incisional Wound Healing in a Rat Model: An Experimental Study

Inflammatory reaction and tensile strength of the abdominal wall after an implant of polypropylene mesh and

polypropylene/poliglecaprone mesh for abdominal wall defect treatment in rats

FIGURE 3 - Photomicrograph of histological cut with septa of the wall of the EG on the seventh day, showing moderately intense inflammatory infiltration and vascular congestion (HE – x400).

Inflammatory reaction and tensile strength of the abdominal wall after an implant of polypropylene mesh and

polypropylene/poliglecaprone mesh for abdominal wall defect treatment in rats

FIGURE 4 – Photomicrograph of a histological cut of the wall of the EG after 56 days, with discreet inflammatory reaction (HE – x200).

Abdominal wall healing in incisional hernia using different biomaterials in rabbits

FIGURE 13 - ULTRAPRO® mesh adhesion to the intestinal wall (HE-40x).

Abdominal wall healing in incisional hernia using different biomaterials in rabbits

FIGURE 14 - PROCEED® mesh in negative image, peripheral giant cell, fibrosis (collagen colored in green), inflammatory infiltrate (Gomori’s trichrome-100x).

Biomechanical and histological evaluation of abdominal wall compliance with intraperitoneal onlay mesh implants in rabbits: A comparison of six different state-of-the-art meshes

Fig. 3. Examples of meshes with or without adhesions. The mesh size was uniformly 30 mm×50 mm.

Fig. 8. H&E histology of the individual meshes. The peritoneal surface is orientated in all images to the top, the fascia to the bottom. The bars in all images equal 1000 m.

Fig. 9. AZAN staining for demonstration of the heterogeneous collagen formation around the implant (arrows). Note that the Physiomesh samples show a loosely wovennetwork of collagen fibers stained in blue. Bars equal 1000 m.

Fig. 10. Representative images of the individual meshes stained for anti-PCNA as proliferation marker. Positive cells in the neosubmesothelial tissue layer are stained inbrown (arrows). Note the absence or light expression of foreign body reaction in the Pysiomesh, Bard composix and Ethicon Proceed meshes. Bars equal 100 m.

Histological and biomechanical evaluation of implantedgraft materials in a rabbit vaginal and abdominal model

Figure 4 A, AF graft 12 weeks after vaginal implantation. New collagen is deposited on the periphery and extends into graft tissue. B, PDX 12 weeks after abdominal implantation. A chronic inflammatory reaction is seen surrounding the graft and also invading a fenestration. Inset box, a foreign body reaction is seen. A thin layer of new collagen is noted above the inflammatory reaction, but this does not invade the graft or fenestration. C, PCPM 12 weeks after abdominal implantation. Collagen and fibroblast infiltration of the graft is demonstrated. Foreign body reaction at mesh site with encapsulation of mesh noted. D, HDA 12 weeks after abdominal implantation. Inflammatory cells surrounding the periphery of graft with minimal collagen or fibroblast infiltration (A, Trichrome stain; B, C, and D, hematoxylin-eosin stain).

Prefabricated flap composed by skin and terminal gastromental vessels. Experimental study in rabbits

Figure 1 On a rabbit from the Group D: (A) Omental pedicle attached to the cutaneous aponeurotic muscle (CAM) in the firstoperatory phase. (B) The flap and the omental pedicle in the second operatory phase (p). (C) Analysis of the flaps after 56 days ofthe last procedure performed. (D) Slide imunostained with anti-CD31 to assess the microvascular density (amplification of 250).

A randomized controlled experimental study comparing chitosan coated polypropylene mesh and Proceed™ mesh for abdominal wall defect closure

Fig. 1. Omental adhesions in the Chitosan and Proceed group.

Fig. 3. High magnification view of histologic section of musculofascial e implant (chitosan) interface showing chitosan fibres with surrounding inflammatory cells.

Fig. 4. High magnification view of histologic cross-section examination of musculofascial e implant interface showing Proceed™ mesh fibers with surrounding inflammatory cells.

Histological response to and immunogenicity of different material patches implanted in rabbit abdominal walls

Figure 1 Histological changes in the UBM (A), cross-linked UBM (B), PP mesh (C), composite cross-linked UBM (D), composite UBM (E),and sham (F) groups.H&E staining; original magnification, × 400. Scale, 10.0 μ m.

1 week 2 weeks 4 weeks 8 weeks 12 weeks

Evaluation of Healing Intervals of Incisional Skin Woundsof Goats Closed with Three Suture Patterns

Fig 2: At day 7 post-surgery, Arrow indicating neutrophilic infiltration ,with markedreepithelization in SC group compared to FI and SI, the inflammatory cells at papillary dermis inSC were dominated by lymphocytes (L), while FI and SI were dominated with neutrophils (N).Slides were stained with with with Masson's trichrome stain: X200 objective magnification, scalebar 100μ.

Comparative study of suture and cyanoacrylates in skin closure of rats

FIGURE 2 - Following wound closure with ECA (7th POD), superficial epidermis is closed (top arrow), while a deeper cavity remains (bottom arrow) (200x)

FIGURE 5 - Fibrosis was apparent in the sutured wound on the 21st POD (200x)

FIGURE 4 - Adhesive fragment in granuloma in wound closed by ECA, 7th POD (1000x)

Keloid and Hypertrophic Scars: Comparative Histopathological and Immunohistochemical Study

Fig. 1. Hypertrophic scar 20 X showing epidermal flattening with scarring of papillary dermis and horizontally oriented wavy collagen fibers.

Fig. 2. Hypertrophic scar 40X showing epidermal disarray.

Fig. 3. Keloid scar 40 X showing basal cell vacuolar change.

Fig. 4. Keloid scar 40 X showing abnormally large dense, broad, glassy, eosinophilic, focally fragmented complexes, arranged haphazardly.

Fig. 5. Hypertrophic scar 40 X showing nodules of fibrillary collagen of fairly regularthickness.

Fig. 6(a). Keloid scar 40 X showing broad glassy collagen.

Fig. 6(b). Keloid scar 60 X showing α-SMA expressing myofibroblasts with glassy collagen.

Fig. 6(c). Keloid scar 40 X showing diffuse positivity for α-SMA expressing myofibroblasts.

Keloid and Hypertrophic Scars: Comparative Histopathological

and Immunohistochemical Study

Fig. 7. Hypertrophic scar 40 X showing absence of α-SMA expressing myofibroblasts.Note vertical blood vessels at the margins of collagen nodules.

Fig. 8. Keloid scar 20 X showing advancing edge below the epidermis.

Fig. 9. Keloid scar 40 X showing chronic inflammatory infiltrate and mast

Comparison of the Histological Morphology between

Normal Skin and Scar Tissue

Fig. 1 Differences in the histology of normal skin and scar tissue HE staining (A, B) and Masson staining (C, D) were performed to visualize the histological structure in normal skin and scar. Scar tissue was composed of thick epidermis and dermis with tough collagenous fibers, and its histological structure differed from the normal skin. E and F: measurement of epidermis thickness of normal skin and scar tissue. **P<0.01 vs. normal skin. Scale bar =50 μm (A–E)

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