ambr® 15 cell cultureThe Industry Standard Single-UseAdvanced Micro Bioreactor System

- Improves early clone selection decisions by providing a predictive bioreactor model

with pH and DO control

- Is the established industry standard with 50 systems installed in the top 20

biopharma companies

- Increases lab productivity by managing multiple (24 or 48) bioreactor

development experiments in parallel.

- Reduces the cost per experiment by saving substantial amounts on facility space,

capital, labour, media and consumables.

- Helps create better cell lines faster by automating vessel set-up, feeding,

base addition and sampling.

- Simplifies adoption of Quality by Design (QbD) principles

enabling large multifactorial experi mental designs (DoE) in real time.

Bioreactor System for Cell Line Development and Process Optimization

ambr® 15 is an automated microscale bioreactor system that replicates classical laboratory scale bioreactors. The benchtop system comprises disposable micro bioreactor vessels, an automated workstation and user friendly software. ambr 15 offers automated parallel processing and control of 24 or 48 bioreactor experiments using just one operator. Designed to be installed in a standard laminar airflow biological safety cabinet for aseptic operation. ambr 15 provides efficient, consistent and scalable bioreactor experiments compared to classical laboratory bioreactors.*

* See separate ambr 15 Performance Data Sheet

ambr® 15 transforms the way scientists undertake cell line development in the 21st century

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Liquid handling unitFor media, feed and reagent addition plus sampling from the bioreactor vessels. Unit includes dual pipette heads, automated vessel decapper and plate delidder.

Culture stationsEach culture station can run up to 12 micro bioreactor vessels and controls temperature and impeller speed. Gas mixture is controlled and delivered independently to each vessel.

ambr® 15 Combines Disposable Micro Bioreactor Vessels, an Automated Workstation and Easy-to-Use Software

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Sterile working environmentSystem fits within most standard biological safety cabinets.

Disposable tipsSterile disposable tips are used to eliminate cross contamination. Two sizes of high grade tips are available to transfer liquid volumes accurately and precisely.

SoftwareSoftware enables easy experiment construction. It controls and monitors all experiments and records data and events with a full audit trail.

Image shows ambr 15 cell culture, 24 vessel system

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Labware lid managementLids are automatically removed prior to liquid transfer and replaced afterwards.

ambr® 15 cell cultureFully automated parallel processing, control and evaluation of all bioreactor experiments with:

– Online monitoring and closed-loop control of pH and DO

– Independent control of:

– O2

– CO2

– N2

for each vessel

– Full control of impeller speed and bioreactor temperature

– Optional integration with Beckman Coulter Vi-CELL XR or Cedex HiRes cell viability analysers

Micro bioreactor vesselThe core disposable micro bioreactor technology includes sensors, stirring impeller, gas sparging and sample port.

1 Gas sparge tube2 Sample port3 pH and DO sensor spots4 Impeller

ambr® 15 cell culture micro bioreactor vesselMimics the characteristics of a classical lab scale bioreactor to enable optimal cell growth and productivity:

– pH and DO sensors for continuous monitoring and control

– Integral impeller for rapid, efficient mixing

– Sparge tube delivers gas to the impeller mixing zone

– 10 to 15 mL culture volume with sample port to allow addition of reagents & feeds or removal of samples

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Functions

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ambr® 15 automatically controls, feeds and samples 24 or 48 bioreactor vessels in parallel

ambr 15 cell culture can be used as a microscale model to unblock bottlenecks in a range of development applications such as:

– Cell line selection

– Media development

– Media feed strategies

– Process optimization

– QbD and DoE studies

Applications

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Sales and Service ContactsFor further contacts, visit www.sartorius-stedim.com

www.sartorius-stedim.com

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