alternative splicing of the pkc δ gene
DESCRIPTION
Alternative Splicing of the PKC δ Gene. Amanda Morris Umberto napoletano Jesse robinson Dr. Audrey shor Dr. niketa patel. Objective of Project. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/1.jpg)
AMANDA MORRISUMBERTO NAPOLETANO
JESSE ROBINSON
DR. AUDREY SHORDR. NIKETA PATEL
Alternative Splicing of the PKCδ Gene
![Page 2: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/2.jpg)
Objective of Project
To increase understanding of the structure and functions of PKCδ through the use of structural alignment, protein modeling and gene mapping.
![Page 3: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/3.jpg)
History of PKC
First discovered in 1977
Activated by DAG and Calcium Some exceptions
3 classes Classical Novel Atypical
http://www.studyblue.com/notes/note/n/pharmacology-test-2/deck/45321
![Page 4: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/4.jpg)
PKCδ
Novel PKC Calcium independent
11 isoforms PKCδI and VIII are
human variantsOpposing functions
PKCδI: Apoptosis PKCδVIII: Pro-survival
![Page 5: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/5.jpg)
PKCδ
http://atlasgeneticsoncology.org/Genes/PRKCDID42901ch3p21.html
![Page 6: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/6.jpg)
Opposing Functions
Alternative splicing Addition of 31 nucleotides between exons 10
and 11Interruption of hinge region
Cleavage necessary for activation
http://www.jbc.org/content/285/34/25987
![Page 7: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/7.jpg)
Retinoic Acid and SC35
RA promotes alternative splicing SC35 Includes 93 nucleotides from intron PKCδVIII is created
http://www.jbc.org/content/285/34/25987
![Page 8: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/8.jpg)
Model
![Page 9: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/9.jpg)
Objective of Instructional Materials
To improve the understanding of how alternative splicing and post-translational modifications can alter the function of PKCδ isoforms.
![Page 10: Alternative Splicing of the PKC δ Gene](https://reader035.vdocuments.mx/reader035/viewer/2022062315/56815793550346895dc5273a/html5/thumbnails/10.jpg)
Gene Map
Obtained mRNA sequence from NCBI Additional nucleotides from NCBI
Protein sequence “hidden” across 3 reading frames
Each frame was aligned (RNA,1,2,3)Instructional guidelines
Protein Sequence mRNA sequence Differing functions