algal biotechnology

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Algal Biotechnology, algae , aplication lecture for 275511 หลักการและการปฏิบัติทางเทคโนโลยีชีวภาพ

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Phy cobi ot echnol ogyPhy cobi ot echnol ogy . . Sc 3202 E-mail:Neti_nu@yahoo.comSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com . 2549. . . 2. . . 2547. . . . . 2549. . . . 2548. . . Lee, E. Robert. 1999. Phycology 3rdeds . Cambridge University Press. UKAnderson A. Robert, 2005, Algal culturing (Eds) :A Book for All Phycologists. Elsevier.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comAlgaeAlgaePhykosPhykosMicroscopic organism thallusMicroalgae macroalgaeAlgology = Algae + logos (logy)Phycology = Phykos + logosSeaweed School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com- autotrophic organisms - microscopic organisms macroscopicorganisms thallusMacroscopic organismsmicroscopic organismsSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com- unicellular organism (single cell)- multicellular organism colony- filament - unbranched filament - branched filament- siphon- thallusSize Size 1 micrometer -----------------------------------> 700 feetSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com bladestiperhizoidleafstemrootSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com geological time table Precambrian blue green algae(6,000 ) bacteria Cambrian(600 )green algaered algaebrown algaeyellow algaeetc.land plants, , School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comEvolut ionar y t r eeBl ue gr een al gaeRed algaeGreen algaeYel l ow al gaeBr ow n al gaeEukaryot eProkaryot eCyanobact er i aSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com1. Cyanophyta - bluegreen algae2. Chlorophyta - green algae3. Charophyta - stoneworts4. Euglenophyta - euglenoids5. Phaeophyta - brown algae6. Chrysophyta - golden algae- yellow green algae- diatoms7. Pyrrhophyta - dinoflagellates8. Cryptophyta - cryptomonads9. Rhodophyta - red algae: 9 DivisionsBold & Wynne (1978)Bold & Wynne (1978)School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comDi vi si on Cyanophy t aDi vi si on Cyanophy t aAnabaena sp. Spirulina sp. Oscillatoria sp.Chroococcus sp. Merismopedia sp. Microcystis sp10School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comDi vi si on Chl or ophy t aDi vi si on Chl or ophy t aSpirogyra sp. Cladophora sp. Acetabularia sp.Scenedesmus sp. Pediastrum sp. Micrasterias sp.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comDi vi si on Charophyt aDi vi si on Charophyt aChar a sp. Ni t el l a sp. Tol ypel l a sp.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comPhacus sp. Euglena sp. Colacium sp.Lepocinclis sp. Trachelomonas sp. Strombomonas sp.Di vi si on Eugl enophyt aDi vi si on Eugl enophyt aSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comDi vi si on Chr y sophy t aDi vi si on Chr y sophy t aDinobryon sp. Synura sp. Isthmochloron sp. Pinnularia sp. Cyclotella sp. Gomphonema sp.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comDi vi si on Pyrr hophyt aDi vi si on Pyrr hophyt aCeratium sp. Cystodinium sp. Gymnodinium sp.Peridinium sp.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comDi vi si on Cr ypt ophyt aDi vi si on Cr ypt ophyt aChroomonas sp. Rhodomonas sp.Cryptomonas sp.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comDi vi si on Phaeophyt aDi vi si on Phaeophyt aDi ct yot a sp.Padi na sp.Sar gassum sp. Fucus sp.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comDi vi si on Rhodophyt aDi vi si on Rhodophyt aBat r achosper mum sp.Si l odot i a sp.Por phyr a sp. Gr aci l ar i a sp.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com1.1. ((pigmentpigment))- Chlorophyll ( a b c d )- Carotinoid ( 60 types)- Alpha,beta-Carotene- Xanthophyll- fucoxanthin- Phycobilin- Phycoerythrin- Phycocyanin- AllophycocyaninSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com2.2. - - - Cellulose Xylan, Mannan,Alginic acid, Pectin,Chitin, Silica, CalciumPectin/Chitin = delivation & CHOSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com3. 3. - starch- leucosin- laminarin- paramylon - Fat- Oil- Chloresterol- ErgosterolPhot osynt hesi s Phot osynt hesi sCarbohydrateSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com FlagellumFlagellum- adult- sex cellEuglena sp. , Chlamydomonas sp.zoospore OedogoniumSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comApical (/) SubapicalEqualUnequal2 equa 2 apical 1 sub-apica 2-unequalSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comBenthic algae = substrate Epipelic algae = Cladophora sp.Nostoc sp.School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comBrackish water = Benthic = Epipelic = Planktonic = Epiphytic = School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comAlgae Green bacteria Purple bacteria electron reduce O2 photosynthesisO2 photosynthesisCarbon sourceRespirationType of chlorophyllH2O H2S , Inorganic substanceH2S , Organic or Inorganic substance CO2CO2CO2 ,Organic substanceAerobicAnaerobicAnaerobic , facultativea, b, c, d Chlorobium Chlorophyll:a-, c, d , e Bacterio chlorophyll:a,b, c, d , e Algae vs. Photosynthetic bacteriaSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comAlgae vs. Higher plant1. Single cell, Colony, Filament, Siphon, Thallus differentiate tissue organ2. vascular system phloem xylem3. Sex cell 1 cell 4. Zygote ----------------> Embryo School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com 1. ( -procaryote / eukaryote)procaryote Procaryote/eukaryoteeukaryote2. photosyntheticbacteria 3. - 4. 6. - School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com--Environment Environment -- ,, --Economic Economic ,, SCP = single cell proteinSCP = single cell protein--produce Oproduce O2 2--producer producer food chainfood chainproductivity productivity 1. EcosystemSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comRed tideA Noct iluca bloom in t he Sea of Cort ezSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com- - phytoplankton bloom- O2 - EutrophicationSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comEut r ophi cat i on i n t he Chi ang Mai Eut r ophi cat i on i n t he Chi ang Mai Uni v er si t y Reser v oi r , Uni v er si t y Reser v oi r , December 2001 December 2001 Januar y 2002 Januar y 2002School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comI dent i f i cat i on : I dent i f i cat i on : Anabaena cat enul a Anabaena cat enul aSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com-- -- 1. 1. LaminariaLaminaria, , CaulerpaCaulerpa2. 2. ((,, )) :: Padina, Caulerpa, UlvaPadina, Caulerpa, Ulva ()2. FoodSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com (microalgae)(microalgae)Axenic culture (sp. )Mass ProductSmall invertebrateRotifer, CyclopDaphnia, MoinaAquatic animal larvaColoured fish, poultry ManAnimals*Chemical r eagent*Waste water (pr oduct)Fer tilizerFecesetc.FiltrationTablet Mixed foodFeeding forSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.com-- ChlorellaChlorella-- ScenedesmusScenedesmus-- AnkistrodesmusAnkistrodesmus-- SpirulinaSpirulinahigh proteinhigh proteinhigh mineral salthigh mineral salthigh vitaminhigh vitaminhigh carotenehigh caroteneMicroalgaehigh proteinChlorellaScenedesmusAnkistrodesmusSpirulinaSchool of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version www.pdffactory.comChlorellaChlorellaSpirulinaSpirulina1. Cell 1. Cell 1. Cell 1. Cell Centrifuge Centrifuge 2. Cell wall 2. Cell wall 2. Cell wall 2. Cell wall 3. 3. SpirulinaSpirulina3. 3. 4. 4. SpirulinaSpirulina 4. 4. School of Medical Science, University of PhayaoPDF created with pdfFactory Pro trial version