aim detect mouse and human actb gene in two co- cultured cell liens cell lines: mef – mus...
TRANSCRIPT
Cell differentiation basing on ACTB expression
AIM• Detect mouse and human ACTB gene in two co-
cultured cell liens
Cell Lines:MEF – Mus muscullus embroynic fibroblasts
Cells infected with SV40 polyomavirus. LargeT antigen(protein) is believed to bind and limit p53 activity – transcription factor responsible for cell death
AIM• Detect mouse and human ACTB gene in two co-
cultured cell liens
Cell Lines:BjhTERT – Homo sapiens sapiens fibroblast
immortalised with hTERT (human telomere reverse transcriptase)
CCTCAATGCTGCTGCTGTACTAC
TGCGTCTATTTAGTGGAGCC
Cy3
Cy5
AGCCTCGCCTTTGCCTTCCTTTTACGACCTCAATGCTGCTGCTGTACTACTCTTCGCCCCGCGAGCACAG TTACACCCTTTCTTTGACAATCCGAGTAGTCTTTGTGCGTCTATTTAGTGGAGCCGCAACTATCTTCTTTGACTGTTACTGAGCTGCGTT
No primer given for mouse ACTB padlock. We will use random priming ! (random decamers : 10 bases oligos)